A5066607841- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- Organ Transplantation Techniques and Outcomes
- Prostate Cancer Treatment and Research
- Photoreceptor and optogenetics research
National Eye Institute
2021
National Institutes of Health
2021
Roswell Park Comprehensive Cancer Center
2015
University of Leicester
2012-2014
Mutations in the photoreceptor transcription factor gene cone-rod homeobox (CRX) lead to distinct retinopathy phenotypes, including early-onset vision impairment dominant Leber congenital amaurosis (LCA). Using induced pluripotent stem cells (iPSCs) from a patient with CRX-I138fs48 mutation, we established an vitro model of CRX-LCA retinal organoids that showed defective maturation by histology and profiling, diminished expression visual opsins. Adeno-associated virus (AAV)-mediated CRX...
During mitotic entry centrosomes separate to establish the bipolar spindle. Delays in centrosome separation can perturb chromosome segregation and promote genetic instability. However, interphase are physically tethered by a proteinaceous linker composed of C-Nap1 filamentous protein, rootletin. Linker disassembly occurs at onset mitosis process known as disjunction is triggered Nek2-dependent phosphorylation C-Nap1. Yet mechanistic consequences unknown. Here, we demonstrate that Nek2...
Centrosome duplication is licensed by the disengagement, or ‘uncoupling’, of centrioles during late mitosis. However, arrest cells in G2 can trigger premature centriole disengagement. Here, we show that disengagement results from untimely activation APC/C leading to securin degradation and release active separase. dependent on Plk1-mediated inhibitor, Emi1, but Plk1 also has a second APC/C-independent role promoting Importantly, activity stimulate response hydroxyurea DNA damage-induced cell...
Multi-drug resistance (MDR)-ATP binding cassette (ABC) transporters, ABCB1, ABCC1, and ABCG2 participate in the efflux of steroid hormones, estrogens, androgens, which regulate prostate development differentiation. The role MDR-ABC transporters epithelial proliferation differentiation remains unclear. We hypothesized that epithelium regeneration. Prostate was studied using histology, sphere formation assay, regeneration induced by cycles repeated androgen withdrawal replacement. Embryonic...