A5067861660- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Bladder and Urothelial Cancer Treatments
- Single-cell and spatial transcriptomics
- Skin Protection and Aging
- Epigenetics and DNA Methylation
- Renal and related cancers
- Cancer, Lipids, and Metabolism
- Hormonal and reproductive studies
- Immunotherapy and Immune Responses
- Cutaneous Melanoma Detection and Management
- Nonmelanoma Skin Cancer Studies
- Drug Transport and Resistance Mechanisms
- Cancer, Hypoxia, and Metabolism
- Urinary and Genital Oncology Studies
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Organ Transplantation Techniques and Outcomes
- Angiogenesis and VEGF in Cancer
- Xenotransplantation and immune response
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
Roswell Park Comprehensive Cancer Center
2013-2024
Semmelweis University
2024
Emory University Hospital
2004-2005
Fred Hutch Cancer Center
2005
Emory University
2005
Cancer Research Center
2005
University of North Carolina at Chapel Hill
2004
Baylor College of Medicine
2001-2004
Abstract Background Animal models that closely mimic clinical disease can be exploited to hasten the pace of translational research. To this end, we have defined windows opportunity in transgenic adenocarcinoma mouse prostate (TRAMP) model cancer as a paradigm for designing pre‐clinical trials. Methods The incidence cancer, metastasis, and distribution pathology were examined function time TRAMP mice. expression various markers differentiation characterized. Results develops progressive,...
Cells with sphere forming capacity, spheroid cells, are present in the malignant ascites of patients epithelial ovarian cancer (EOC) and represent a significant impediment to efficacious treatment due their putative role progression, metastasis chemotherapy resistance. The exact mechanisms that underlie EOC drug resistance not clear. Understanding biology cells may contribute identification novel therapeutic opportunities for metastatic EOC. Here we generated from human cell lines primary...
Abstract Malignantly transformed stem cells represent a potential common nidus for the primary cancer and recurrent that arises after treatment failure. Putative prostate tumor in benign malignant human tissue, xenografts, transgenic adenocarcinoma of mouse (TRAMP) model cancer, are defined by expression breast resistance protein (BCRP), marker pluripotent hematopoietic, muscle, neural cells, an absence androgen receptor (AR) protein. Inhibition BCRP-mediated efflux dihydrotestosterone...
In ultraviolet (UV) radiation-exposed skin, mutations fuel clonal cell growth. The relationship between UV exposure and the accumulation of (CMs) correlation CMs skin cancer risk are largely unexplored. We characterized 450 individual-matched sun-exposed (SE) non-SE (NE) normal human samples. number relative contribution were significantly different SE NE areas. Furthermore, we identified hotspots in
Abstract BACKGROUND Cancer stem cells are defined by their self‐renewal and multi‐potential capabilities hypothesized to be the source of primary recurrent cancers. The cell properties pluripotency in embryonic germ regulated Oct4A, a splice variant POU5F1 (Oct3/4) gene, while function alternative variant, Oct4B, is unknown. Rare that express Oct4 were identified several somatic cancers, however, differential contributions Oct4A Oct4B variants not determined. METHODS expression...
Following castration, the transgenic adenocarcinoma of mouse prostate (TRAMP) model demonstrates rapid development SV40-Tag-driven poorly differentiated tumors that express neuroendocrine cell markers. The population dynamics within prostates castrated TRAMP mice were characterized by analyzing incorporation 5-bromodeoxyuridine (BrdUrd) and expression SV40-Tag, synaptophysin, androgen receptor (AR). Fourteen days postcastration, remaining epithelial cells nonproliferative lacked detectable...
The efflux of Hoechst 33342 by ATP-binding cassette protein G2 (ABCG2) membrane pump allows reproducible identification a subpopulation cells flow cytometric analysis termed the “side population” (SP). SP identified constitutive contains stem/progenitor cell population from bone marrow and many solid organs, including prostate. DyeCycle Violet (DCV) is permeable, fluorescent vital dye that intercalates into DNA substrate for ABCG2-mediated efflux. Therefore, DCV was evaluated in this study...
Prostate stem cells (PSC) are characterized by their intrinsic resistance to androgen deprivation therapy (ADT), possibly due the lack of receptor (AR) expression. PSCs ADT and PSC expansion in castration resistant prostate cancer (CRPC) has sparked great interest using differentiation as an adjuvant ADT. Understanding mechanisms, which maintain undifferentiated phenotype, thus important implications therapy. In prostate, ATP binding cassette sub-family G member 2 (ABCG2) transporters,...
Abstract BACKGROUND The aim of this study was to elucidate how changes in temporal and spatial expression patterns individual components the fibroblast growth factor (FGF) signaling axis correlate with prostate cancer‐associated angiogenesis contribute progression disease. METHODS specific FGF ligands receptors were characterized by immunoblot, situ hybridization, immunohistochemical analyses transgenic adenocarcinoma mouse (TRAMP) model. RESULTS We detected high molecular weight isoform...
Abstract BACKGROUND Neuroendocrine (NE) cell differentiation is proposed to facilitate prostate cancer (CaP) recurrence following androgen deprivation through secretion by NE cells of growth factors and neuropeptides that support survival proliferation CaP vasculature. METHODS The effect on tumor in the CWR22 model human was determined immunohistochemical analysis marker synaptophysin Ki67. RESULTS A significant increase number observed tumors between 28 66 days after castration compared...
Abstract BACKGROUND All‐trans retinoic acid (ATRA) promotes terminal differentiation in epithelial cells and anti‐angiogenesis thus, may have beneficial effects an intervention therapy for prostate cancer. METHODS We used the autochthonous spontaneous transgenic adenocarcinoma of mouse (TRAMP) model system to test ability ATRA prevent initiation progression cancer a pre‐clinical setting. RESULTS Initial studies demonstrated that exposure TRAMP‐derived C2N tumor vitro decreased total viable...
Abstract Transgenic spontaneously occurring and transplantable xenograft models of adenocarcinoma the prostate (CaP) are established tools for study CaP progression metastasis. However, no animal model has been characterized that recapitulates response human vascular compartment to evolving tumor microenvironment during progression. We report primary xenografts noninvolved areas peripheral zone transplanted athymic nude mice provide a unique angiogenesis in an intact tissue microenvironment....
Androgen deprivation causes a reduction of blood flow in the prostate gland that precedes temporally apoptosis epithelium. The acute response endothelial cells to androgen suggested they represent primary target for androgen. However, rat were reported not express receptor (AR), and role axis human cell (HPEC) homeostasis was poorly characterized. In this study AR expression detected HPEC vivo clinical specimens benign cancer, function as transcription factor demonstrated xenografts tissue...
Stem cell enrichment provides a tool to examine prostate stem cells obtained from benign and malignant tissue. Functional assays can enrich based on common phenotypes, such as high ATP binding cassette (ABC) transporter mediated efflux of Hoechst substrates (side population assay). This functional assay is upon mechanisms that protect environmental insult thus contributing the survival protection population. We have isolated analyzed digested twelve clinical specimens side assay. Prostate...
High expression of aldehyde dehydrogenase1A1 (ALDH1A1) is observed in many organs and tumors may identify benign cancer stem cell populations. In the current study, characteristics were determined cells isolated from human prostate lines clinical specimens based upon ALDEFLUOR™ assay. Cells on assay compared to ATP binding cassette transporter G2 (ABCG2) activity using side population To test for self-renewal multipotency, with high low ALDH1A1 activity, (ALDHHi ALDHLow), recombined rat...
Photosensitivity to structurally diverse drugs is a common but under-reported adverse cutaneous reaction and can be classified as phototoxic or photoallergic. Phototoxic reactions occur when the skin exposed sunlight after administering topical systemic medications that exhibit photosensitizing activity. These depend on dose of medication, degree exposure ultraviolet light, type sufficient distribution volume. Accurate prediction incidence response severity challenging due paucity...
Abstract BACKGROUND Deregulation of the cell cycle can be viewed as both cause and consequence cancer. Cyclin expression regulates progression through although some cyclins have been examined in prostate cancer, spatial temporal changes these molecules during autochthonous disease has not fully explored. METHODS Expression patterns cyclin dependent kinases different stages spontaneous TRAMP model were by RNAse protection assay, Western blot analysis, immunohistochemistry. RESULTS...
The transgenic adenocarcinoma mouse prostate (TRAMP) model is a paradigm that closely mimics the progression of clinical cancer. We have previously reported MUC18, cell adhesion molecule in Ig gene superfamily, marker as well an important mediator for metastatic potential human cancer cells. In this study we investigated possible correlation increased MUC18 expression with malignant TRAMP model.We used immunohistochemistry, Western blot and reverse transcriptase-polymerase chain reaction...
Abstract INTRODUCTION The phenotypic plasticity of the human prostate stem cell within tissue was examined to determine response changes in androgenic environment. METHODS Prostate xenografts were transplanted into athymic nu / mice implanted with testosterone pellets, allowed establish for 1 month time point, hosts castrated and pellets removed, following androgen deprivation, stimulated 2 days induce proliferation residual population cells (2‐month point). RESULTS Glands benign harvested...