A5103062465- interferon and immune responses
- MicroRNA in disease regulation
- Immune cells in cancer
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Dermatology and Skin Diseases
- Psoriasis: Treatment and Pathogenesis
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Zebrafish Biomedical Research Applications
- COVID-19 Clinical Research Studies
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- Asthma and respiratory diseases
- Pregnancy and preeclampsia studies
- Circular RNAs in diseases
- Estrogen and related hormone effects
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- Skin Protection and Aging
- Cytokine Signaling Pathways and Interactions
- COVID-19 diagnosis using AI
Zhejiang Hospital
2023-2024
Zhejiang University
2015-2024
Sir Run Run Shaw Hospital
2023
Guangdong Ocean University
2022
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2022
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)
2022
Zhejiang Center for Disease Control and Prevention
2022
Guangzhou Medical University
2020-2021
First Affiliated Hospital of Guangzhou Medical University
2020-2021
Guangzhou University
2021
Abstract Myeloid-derived suppressor cells (MDSCs) potently suppress the anti-tumor immune responses and also orchestrate tumor microenvironment that favors angiogenesis metastasis. The molecular networks regulating accumulation functions of tumor-expanded MDSCs are largely unknown. In this study, we identified microRNA-494 (miR-494), whose expression was dramatically induced by tumor-derived factors, as an essential player in activity targeting phosphatase tensin homolog (PTEN) activation...
MicroRNAs are small non-coding RNA molecules that regulate gene expression by either translational inhibition or mRNA degradation. play pivotal roles in the regulation of both innate and adaptive immune responses, including TLR-triggered inflammatory response. Here we reported microRNA-223 (miR-223) was significantly decreased murine macrophages during activation lipopolysaccharide (LPS) poly (I∶C) stimulation. The inducible miR-223 down-regulation resulted signal transducer activator...
Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that crucial for dampening the inflammatory response after pathogen invasion, and was found to be produced by macrophages exposure lipopolysaccharide (LPS). It remains unclear whether microRNA-mediated regulatory mechanism involved in LPS-induced IL-10 production. Here we reported miR-98 expression significantly decreased following LPS stimulation. We also targets 3'untranslated region of transcript. Overexpression inhibited...
Resident and inflammatory mononuclear phagocytes (MPhs) with functional plasticity in the intestine are critically involved pathology of bowel diseases (IBDs), mechanism which remains incompletely understood. In present study, we found that increased expression E3 ligase F-box WD repeat domain–containing 7 (FBXW) inflamed was significantly correlated IBD severity both human mouse models. Myeloid Fbxw7 deficiency protected mice from colitis induced by dextran sodium sulfate (DSS) or...
Abstract The malignancy of colorectal cancer (CRC) is connected with inflammation and tumor-associated macrophages (TAMs), but effective therapeutics for CRC are limited. To integrate therapeutic targeting tumor microenvironment (TME) reprogramming, here we develop biocompatible, non-covalent channel-type nanoparticles (CNPs) that fabricated through host-guest complexation self-assemble mannose-modified γ-cyclodextrin (M-γ-CD) Regorafenib (RG), RG@M-γ-CD CNPs. In addition to its carrier...
Chemo-immunotherapy is an important tool to overcome tumor immune suppression in cancer immunotherapy. Herein, we report a surface-layer (S-layer) protein-enhanced immunotherapy strategy based on cell membrane-coated S-CM-HPAD nanoparticles for the effective malignant therapy and metastasis inhibition. The NPs could effectively deliver antigen, DOX, immunoadjuvant homotypic by targeting ability of coated membrane. In addition its induce death, loaded DOX enhance response inhibition...
A dietary deficiency of vitamin is associated with cardiovascular abnormalities in avian and murine systems. Retinoic acid (RA) the active metabolite whether it directly regulates mammalian blood vessel formation has not been determined investigated herein. We used mice rendered RA-deficient via targeted deletion retinaldehyde dehydrogenase 2 (Raldh2(-/-)), enzyme required to produce RA embryo. Histological examination at E8.0-8.5, prior cardiac function systemic circulation, revealed that...
We previously demonstrated that during vascular morphogenesis, retinoic acid (RA) is required for the control of endothelial cell proliferation and capillary plexus remodeling. Herein, we investigate mechanisms by which RA regulates these processes in yolk sac. found although enzyme production early embryogenesis, retinaldehyde dehydrogenase-2 (Raldh2), was expressed visceral endoderm, receptors α1 α2 were cells mesoderm, indicating they are direct targets RA. In Raldh2 -/- embryos, there...
Viruses can escape from host recognition by degradation of RIG-I or interference with the signalling to establish persistent infections. However, mechanisms which cells stabilize protein for avoiding its are largely unknown. We report here that, upon virus infection, E3 ubiquitin ligase FBXW7 translocates nucleus into cytoplasm and stabilizes RIG-I. interacts SHP2 mediates ubiquitination SHP2, thus disrupting SHP2/c-Cbl complex, degradation. When infected VSV influenza A virus, conditional...
The high proliferative rate of tumor cells leads to metabolic needs distinct from those their normal counterparts. An embryonic- and tumor-specific isoform the enzyme pyruvate kinase M2 (PKM2) is overexpressed in cancer increase use glycolytic intermediates for macromolecular biosynthesis growth. We report that Cyclosporin A (CsA) can regulate expression activity PKM2 breast cell lines MCF-7, MDA-MB-435 MDA-MB-231. was found be highly expressed three compared primary cells. Treatment with...
Effective recognition of viral infection and subsequent triggering antiviral innate immune responses are essential for the host defense, which is tightly regulated by multiple regulators, including microRNAs. Previous reports have shown that some microRNAs induced during virus participate in regulation response. However, whether type I IFN response miR-223 still unknown. Here, we reported vesicular stomatitis (VSV) significant up-regulation murine macrophages. We observed overexpression...
The presence of cancer stem cells (CSC), which possess the ability self-renewal and initiation, is correlated with poor prognosis drug resistance breast patients. But molecular regulatory networks for maintenance CSC function still remain unclear. Here, we identified that an estrogen-inducible gene FXYD3, whose expression significantly upregulated in ER+ CSCs, a critical player regulating function. FXYD3 amplification crucial mediating tamoxifen cells. Interestingly, also find cell-related...
Abstract Background Tamoxifen resistance remains a significant clinical challenge for the therapy of ER-positive breast cancer. It has been reported that upregulation transcription factor SOX9 in ER + recurrent cancer is sufficient tamoxifen resistance. However, mechanisms underlying regulation remain largely unknown. Methods The acetylation level was detected by immunoprecipitation and western blotting. expressions HDACs SIRTs were evaluated qRT-PCR. Cell growth measured performing MTT...
Abstract For nearly 40 years functional studies of the mouse quaking gene ( qkI ) have focused on its role in postnatal central nervous system during myelination. However, homozygous lethality a number ENU‐induced alleles reveals that has critical embryonic development prior to start In this article, we show previously unsuspected and essential blood vessel development. Interestingly, found , nonsecreted protein, is expressed yolk sac endoderm, adjacent mesodermal site developing islands,...
Transforming growth factor-β induces a smooth muscle cell phenotype in undifferentiated mesenchymal cells. To elucidate the mechanism(s) of this phenotypic induction, we focused on molecular regulation muscle-γ-actin, whose expression is induced at late stages differentiation and developmentally restricted to lineage. muscle-γ-actin protein, cytoskeletal localization, mRNA Smooth promoter-luciferase reporter activity was enhanced by transforming factor-β, deletion analysis revealed that CArG...
Keywords: Extracellular pH, Ioversol, CEST MRI, Hepatic carcinoma, hemangioma. Purpose: In this study, we aimed to use 3T magnetic resonance imaging (MRI), which is clinically available, determine the extracellular pH (pHe) of liver tumors and prospectively evaluate ability chemical exchange saturation transfer (CEST) MRI distinguish between benign malignant tumors. Methods: Different radiofrequency irradiation schemes were assessed for ioversol-based measurements at 3T. effects quantified...