A5034446177- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Malaria Research and Control
- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Morphological variations and asymmetry
- Viral Infections and Vectors
- Research on Leishmaniasis Studies
- Sleep and Wakefulness Research
- Economic and Environmental Valuation
- Dengue and Mosquito Control Research
- Cannabis and Cannabinoid Research
- Travel-related health issues
- Trypanosoma species research and implications
- Wildlife Ecology and Conservation
- Sleep and related disorders
Fundação Oswaldo Cruz
2015-2024
Florida College
2022
University of Florida
2022
Traditional vector control approaches such as source reduction and insecticide spraying have limited effect on reducing Aedes aegypti population. The endosymbiont Wolbachia is pointed a promising tool to mitigate arbovirus transmission has been deployed worldwide. Models predict rapid increase the frequency of Wolbachia-positive Ae. mosquitoes in local settings, supported by cytoplasmic incompatibility (CI) high maternal rate associated with wMelBr strain.Wolbachia strain was released for 20...
The chemical control of the mosquito Aedes aegypti, major vector dengue, is being seriously threatened due to development pyrethroid resistance. Substitutions in 1016 and 1534 sites voltage gated sodium channel (AaNaV), commonly known as kdr mutations, confer with knockdown Our aim was evaluate allelic composition natural populations Brazilian Ae. aegypti at both sites.The AaNaV IIIS6 region cloned sequenced from three populations. Additionally, individual mosquitoes 30 throughout country...
Background In the tropics, utilization of insecticides is still an important strategy for controlling Aedes aegypti, principle vector dengue, chikungunya and Zika viruses. However, increasing insecticide resistance in Ae. aegypti populations might hinder efficacy on a long-term basis. It will be to understand dynamics evolution by assessing its frequency mechanisms which it occurs. Methodology/Principal findings The status four Brazilian was monitored. Quantitative bioassays with major...
Background Aedes-borne arboviruses have emerged as an important public health problem worldwide and, in Mozambique, the number of cases and its geographical spread been growing. However, information on occurrence, distribution ecology Aedes aegypti Ae. albopictus mosquitoes remain poorly known country. Methods Between March April 2016, a cross-sectional study was conducted 32 districts Mozambique to determine breeding sites albopictus. Larvae pupae were collected from total 2,807...
The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been lack rapid and affordable tests pathogen detection in mosquitoes. We show first time that near-infrared spectroscopy (NIRS) is rapid, reagent-free, cost-effective tool can be used to noninvasively detect ZIKV heads thoraces intact Aedes aegypti mosquitoes with prediction accuracies 94.2 99.3%...
Mosquitoes that carry Wolbachia endosymbionts may help control the spread of arboviral diseases, such as dengue, Zika and chikungunya. frequencies systematically increase only when frequency-dependent advantage due to cytoplasmic incompatibility exceeds frequency-independent costs, which be intrinsic and/or can associated with genetic background into are introduced. Costs depend on field conditions environmental pesticide load. Introduced mosquitoes need adequate protection against...
ABSTRACT Two Wolbachia strains, w Mel and AlbB, have been transinfected into Aedes aegypti mosquitoes for population replacement with the aim of reducing dengue transmission. Epidemiological data from various endemic sites suggest a pronounced decrease in transmission after implementing this strategy. In study, we investigated impact strains AlbB on Ae. fitness common genetic background. We found that females infected strain exhibited several significant differences compared those strain....
Chemical control is still a major strategy to constrain vector density and mitigate pathogen transmission. However, insecticide overuse poses high selective pressure, favouring the spread of resistance alleles in natural populations. In an insecticide-free environment, fitness cost expected resistant insects when compared susceptible counterparts. This study investigates whether organophosphate (temephos) pyrethroid (deltamethrin) associated with traits four Aedes aegypti wild populations...
Strategies to minimize dengue transmission commonly rely on vector control, which aims maintain Ae. aegypti density below a theoretical threshold. Mosquito abundance is traditionally estimated from mark-release-recapture (MRR) experiments, lack proper analysis regarding accurate spatial distribution and population density. Recently proposed strategies control vector-borne diseases involve replacing the susceptible wild by genetically modified individuals’ refractory infection pathogen....
America`s Pandemic of Zika virus (ZIKV) started soon after the first autochthonous cases in Latin America. Although Aedes aegypti is pointed as primary vector America, little known about fitness cost due to ZIKV infection. We investigated effects infection on life-history traits Ae. females collected three districts Rio de Janeiro, Brazil (Barra, Deodoro and Porto), equidistant ~25Km each other. mosquitoes were classified infected (a single oral challenge with ZIKV) superinfected (two...
Abstract Deployment of Wolbachia to mitigate dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) transmission is ongoing in 12 countries. One way assess the efficacy releases determine invasion rates within wild population Aedes aegypti following their release. Herein we evaluated accuracy, sensitivity specificity Near Infrared Spectroscopy (NIRS) estimating time post death, ZIKV-, CHIKV-, -infection trapped dead female Ae. mosquitoes over a period 7 days. Regardless infection type,...
Mosquitoes carrying the endosymbiont bacterium Wolbachia have been deployed in field trials as a biological control intervention due to effects on reducing transmission of arboviruses. We performed mark, release and recapture (MRR) experiments using an internal marker with daily collections BG-Traps during first two weeks releases Rio de Janeiro, Brazil. The MRR design allowed us investigate critical parameters that determine whether would successful invade population: probability survival...
To eliminate malaria, scalable tools that are rapid, affordable, and can detect patients with low parasitemia required. Non-invasive diagnostic reagent-free, affordable would also provide a justifiable platform for testing malaria in asymptomatic patients. However, non-invasive surveillance techniques remain gap. Here, we show near-infrared Plasmodium absorption peaks acquired non-invasively through the skin using miniaturized hand-held spectrometer. Using spectra from ear, these machine...
(1) Background: The deployment of the bacterium Wolbachia to reduce arbovirus transmission is ongoing in several countries worldwide. When Wolbachia-carrying Aedes aegypti are released and established field, females may feed on dengue-infected hosts. effects simultaneous exposure life-history traits Ae. wMel strain dengue-1 virus DENV-1 remain unclear. (2) Methods: We monitored 4 groups (mosquitoes with either or Wolbachia, coinfected as well negative controls) estimate survival, oviposition...
Several countries have been using Wolbachia deployments to replace highly competent native Aedes aegypti populations with Wolbachia-carrying mosquitoes lower susceptibility arboviruses such as dengue, Zika, and chikungunya. In Rio de Janeiro, started in 2015 still present a moderate introgression modest reduction dengue cases humans (38%). Here, we evaluated the vector competence of wild-type wMel-infected Ae. Brazilian genetic background investigate whether virus leakage could contribute...
Experiments involving mosquito mark-release-recapture (MRR) design are helpful to determine abundance, survival and even recruitment of populations in the field. Obstacles MRR protocols include marking limitations due small individual size, short lifespan, low efficiency capturing devices such as traps, removal upon capture. These usually make analysis restricted only abundance estimation or a combination survivorship, often generate great degree uncertainty about estimations.We present set...
Chagas disease is a neglected tropical caused by Trypanosoma cruzi parasite with an estimated 70 million people at risk. Traditionally, presence in triatomine vectors detected through optical microscopy which can be low sensitivity or molecular techniques costly endemic countries. The aim of this study was to evaluate the ability reagent-free technique, Near Infrared Spectroscopy (NIRS) for rapid and non-invasive detection T. Triatoma infestans body parts wet/dry excreta samples insect. NIRS...
The transmission of dengue (DENV) and Zika (ZIKV) has been continuously increasing worldwide. An efficient arbovirus surveillance system is critical to designing early-warning systems increase preparedness future outbreaks in endemic countries. Near Infrared Spectroscopy (NIRS) a promising high throughput technique detect infection Ae. aegypti with remarkable advantages such as cost time effectiveness, reagent-free, non-invasive nature over existing molecular tools for similar purposes,...
Understanding aspects related to the physiology and capacity of vectors is essential for effectively controlling vector-borne diseases. The sand fly Lutzomyia longipalpis has great importance in medical entomology disseminating Leishmania parasites, causative agent Leishmaniasis, one main neglected diseases listed by World Health Organization (WHO). In this respect, it necessary evaluate transmission potential species success vector control interventions. Near-infrared spectroscopy (NIRS)...
Abstract Accurate identification of mosquito species is essential for effective vector control and mitigation mosquito-borne disease outbreaks. Traditional morphological requires highly specialized personnel time-consuming, while molecular techniques can be cost-effective dependent on comprehensive genetic information. Wing geometric morphometry has emerged as a promising alternative, leveraging detailed measurements wing shapes vein patterns to distinguish between detect intraspecies...