The purpose of this study was to determine whether exogenous zinc prevents cardiac reperfusion injury by targeting the mitochondrial permeability transition pore (mPTP) via glycogen synthase kinase-3beta (GSK-3beta). treatment H9c2 cells with ZnCl2 (10 microM) in presence ionophore pyrithione for 20 min significantly enhanced GSK-3beta phosphorylation at Ser9, indicating that can inactivate cells. effect on activity blocked phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 but not...

Our previous study demonstrated that zinc prevents cardiac reperfusion injury by targeting the mitochondrial permeability transition pore (mPTP) via Akt and glycogen synthetase kinase 3beta (GSK-3beta). We aimed to address mechanism which activates Akt. Treatment of H9c2 cells with ZnCl(2) (10 microM) in presence ionophore pyrithione (4 for 20 min enhanced phosphorylation (Ser(473)), indicating can rapidly activate Zinc did not alter either phosphatase tensin homolog deleted on chromosome 10...

Safe hydrogel delivery requires stiffness-matching with host tissues to avoid iatrogenic damage and reduce inflammatory reactions. Hydrogel-encapsulated cell is a promising combinatorial approach spinal cord injury therapy, but lack of in vivo clinical stiffness measurements barrier their use clinics. We demonstrate that ultrasound elastography - non-invasive, clinically established tool can be used measure intraoperatively canines spontaneous injury. In line recent experimental reports, our...

Background T-tubules are invaginations of the sarcolemma that play a key role in excitation-contraction coupling mammalian cardiac myocytes. Although t-tubules were generally considered to be effectively absent atrial myocytes, recent studies on cells from larger mammals suggest may more numerous than previously supposed. However, degree heterogeneity between cardiomyocytes extent t-tubule network remains unclear. The aim present study was investigate pig myocytes comparison with ventricular...

Significance Sudden cardiac death in heart failure is a major unsolved clinical problem that linked to the development of spontaneous arrhythmia. Early afterdepolarizations (EADs) are an arrhythmogenic mechanism, but cellular trigger for EADs unclear. We show reduction synchronous Ca 2+ release early action potential (AP) failing myocytes promotes appearance late sparks which can propagate, forming ripples and waves. These, turn, produce inward sodium–calcium exchange current opposes AP...

Abstract Objective To report signalment, history, indications, complications and outcome for 28 dogs 5 cats in which 34 permanent epicardial pacing leads were surgically placed by transdiaphragmatic approach (32) or intercostal thoracotomy (2). Methods Medical records (2005–2010) reviewed. Signalment, age, species, gender, clinical signs, presence of structural heart disease and/or congestive failure, ECG diagnosis, body weight (<10 >10 kg), overall survival rate recorded. Statistical...

To compare the outcomes of dogs treated at a single institution for extrahepatic congenital portosystemic shunts (CPSS) by thin film banding (TFB) or placement an ameroid constrictor (AC).Retrospective case series.Seventy-six client-owned with CPSS TFB (n = 53) AC 23).Records were reviewed signalment, preoperative, intraoperative, and postoperative management short-term outcomes. Data on second surgeries reviewed. Long-term obtained via owner-directed health-related quality life...

Objective— To report outcome after surgical and interventional radiographic treatment of hepatic arteriovenous fistulae (HAVF) in dogs. Study Design— Retrospective study. Animals— Dogs (n=20) with HAVF. Methods— Medical records dogs HAVF were reviewed. Referring veterinarians owners contacted by telephone. History, clinical signs, biochemical hematologic variables, ultrasonographic angiographic findings, techniques used to correct the HAVF, survival time, follow‐up recorded. Results— Canine...

O bjective : To review the postoperative results and complications associated with urethral sphincter mechanism incompetence managed a static hydraulic sphincter. M ethods Case records telephone owner questionnaire were retrospectively used to assess urinary continence scores (1 – dripping constantly 10 completely dry) presence frequency of complications. R esults Eleven spayed females included. Median score/10 (range) awarded preoperatively was 3 (2 6), at two weeks, three six months 8 (4...

Objectives-Intracorporeal suturing and knot tying can complicate, prolong or preclude minimally invasive surgical procedures, reducing their advantages over conventional approaches.An automated knot-tying device has been developed to speed suture fixation during cardiac surgery while retaining the desirable characteristics of hand-tied surgeon's knots: holding strength visual haptic feedback.A rotating slotted disk (at instrument's distal end) automates overhand throws, thereby eliminating...

A 24-week-old dog was presented with recurrent rectal prolapse because of colonic duplication. Colonic duplication is an extremely uncommon congenital abnormality, only six cases reported in veterinary medicine, one diagnosed at necropsy and five after barium enema, colonoscopy, abdominal ultrasound, exploratory laparotomy either alone or combination. In this case, these techniques failed to identify the abnormality diagnosis ultimately achieved via negative contrast computed tomography. The...

Abstract Objective To report the complications and long‐term outcome of female dogs with intramural ectopic ureter(s) (iEU) undergoing cystoscopic‐guided laser ablation (CLA) determine effect post‐CLA neutering on urinary continence. Study Design Retrospective clinical study. Animals or Sample Population Thirty‐four client‐owned dogs. Methods Medical records that had iEU‐CLA were reviewed. A 10‐point continence score was assigned before, immediately after, at a minimum 12 months...

Abstract Objective To determine the effect of flushing common bile duct (CBD) on hepatobiliary markers and short‐term outcome in dogs undergoing cholecystectomy for management gallbladder mucocele (GBM). Study design Randomized, controlled, prospective study. Animals Thirty‐two client‐owned dogs. Methods Dogs were allocated randomly to either a “flush” group or “non‐flush group.” Flushing was performed normograde fashion, followed by routine cholecystectomy. Data collected included...

Changes in plasma creatinine (Pl-Creat), glucose, total proteins (Pl-TP), creatine kinase (Pl-CK), lactate dehydrogenase, aspartate aminotransferase and packed cell volume were evaluated untrained Beagle dogs before, during after a 60 min race at an average speed of 9 km/h. There was no major effect exercise on those parameters except, when phases are compared, slight increase Pl-TP (max. + 6.4%), moderate but (delayed Pl-CK max. 245%) decrease Pl-Creat -9.6%). It therefore concluded that...

Objectives: To compare the mechanical properties of two different materials for canine median sternotomy closure. Study Design: In vitro biomechanical study. Sample Population: Twelve cadaveric entire sternum and portion associated ribs musculature. Methods: Median leaving manubrium intact was closed using polydioxanone stainless steel wire in a figure eight pattern. Constructs were loaded servohydraulic material testing system displacement compared at loads 125, 150, 200, 250, 300, 350 400...

The purpose of the study was to report postoperative outcome, complications, and long-term follow-up use a static hydraulic urethral sphincter for management mechanism incompetence in female dogs. Medical records were reviewed extract information on (>365 days) outcome data. Telephone owner questionnaire performed assess urinary continence scores (scale 1-10, where 10 is complete continence) presence frequency complications. Twenty dogs included. Mean (±standard deviation) time 1,205.1...

Objectives To report the clinical characteristics and recurrence rate of spontaneous pneumothorax secondary to pulmonary blebs bullae following surgical management in a large cohort dogs. explore potential risk factors for describe outcome. Materials Methods Medical records were retrospectively reviewed cases with managed surgically between 2000 2017. Signalment, presentation, diagnostic imaging, surgery, histopathology findings patient outcomes recorded. Follow‐up was performed via...

Summary Hip dysplasia (HD) scores, based on the five grades, as defined by Fédération Cynologique Internationale, were compared between anaesthetized (group 1, n = 3839) and non‐sedated non‐anaesthetized dogs 2, 1517). Each dog was radiographed in standard ventro‐dorsal hip joint extended position. radiograph evaluated same reader blinded regarding dog's status of anaesthesia. Results showed that there a significant difference prevalence group 1 (22%) with 2 (9%) ( P < 0.005). This result...

Abstract Case Description —A 6-year-old Siberian Husky–mix dog was examined for episodes of collapse. Clinical Findings —Physical examination, echocardiography, abdominal ultrasonography, ECG, and thoracic computed tomography with contrast were performed revealed a 2.5 × 2.3 2.0-cm mass over the pulmonic valve leaflets, resulting in moderate stenosis. Other abnormal findings included systemic hypertension, right bundle branch block, proteinuria, urinary bladder mass. Treatment Outcome...

OBJECTIVE To compare closed and open orchidectomy in dogs the associated complications. METHODS A randomised controlled blinded prospective clinical study of 73 cases was undertaken involving recording all complications during 10 days following fulfilling standardised inclusion criteria. The active variable surgical technique either or orchidectomy. RESULTS Dogs undergoing experienced significantly more complication events than [24 34 (70%) versus 18 39 (46%), P = 0 · 04]. were statistically...