A5029851754- Escherichia coli research studies
- Gut microbiota and health
- Probiotics and Fermented Foods
- Cell Image Analysis Techniques
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Dietary Effects on Health
- Circadian rhythm and melatonin
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Advanced Chemical Sensor Technologies
- Clostridium difficile and Clostridium perfringens research
- Evolutionary Algorithms and Applications
- Gene Regulatory Network Analysis
- Identification and Quantification in Food
- Animal Nutrition and Physiology
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Diverse Scientific Research Studies
- Liver Disease Diagnosis and Treatment
- Biotin and Related Studies
- Diabetes and associated disorders
- Microbial Metabolites in Food Biotechnology
- CCD and CMOS Imaging Sensors
- Food Safety and Hygiene
University of Virginia
2013-2022
Ethanolamine (EA) metabolism is a trait associated with enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 (EHEC). EHEC causes severe bloody diarrhea and hemolytic uremic syndrome. encodes the ethanolamine utilization (eut) operon that allows to metabolize EA gain competitive advantage when colonizing gastrointestinal tract. The eut transcriptional regulator EutR. Genetic studies indicated EutR expression induced by vitamin B12 promotes of operon; however, biochemical...
AraC Negative Regulators (ANR) suppress virulence genes by directly down-regulating AraC/XylS members in Gram-negative bacteria. In this study, we sought to investigate the distribution and molecular mechanisms of regulatory function for ANRs among different bacterial pathogens. We identified more than 200 distributed diverse clinically important gram negative pathogens, including Vibrio spp., Salmonella Shigella Yersinia Citrobacter enterotoxigenic (ETEC) enteroaggregative E. coli (EAEC),...
DNA-paramagnetic silica bead aggregation in a rotating magnetic field facilitates the quantification of DNA with femtogram sensitivity, but yields no sequence-specific information. Here we provide an original description inhibition for detection and RNA manner following loop-mediated isothermal amplification (LAMP). The fragments generated via LAMP fail to induce chaotrope-mediated aggregation; however, due their ability passivate surface, they effectively inhibit by longer 'trigger' DNA. We...
ABSTRACT Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is a foodborne pathogen that causes bloody diarrhea and hemolytic uremic syndrome throughout the world. A defining feature of EHEC pathogenesis formation attaching effacing (AE) lesions on colonic epithelial cells. Most genes code for AE lesion formation, including type three secretion system (T3SS) effectors, are carried within chromosomal pathogenicity island called locus enterocyte effacement (LEE). In this study, we report...
Polymeric immunoglobulin receptor (pIgR) transport of secretory A (SIgA) to mucosal surfaces is thought promote gut integrity and immunity Salmonella enterica serovar Typhimurium (S. Typhimurium), an invasive pathogen in mice. To elucidate potential mechanisms, we assessed intestinal barrier function both oral systemic S. virulence pIgR knockout (KO) wildtype (WT) mice.In uninfected animals, harvested jejunal segments for Ussing chamber analyses transepithelial resistance (TER); mesenteric...
ABSTRACT Background and aims The circadian clock orchestrates ~24-hour oscillations of gastrointestinal (GI) epithelial structure function that drive diurnal rhythms in the composition, localization, metabolism gut microbiota. Here, we use experimental computational approaches enteroids to reveal reciprocal effects microbial metabolites on intestinal timekeeping by an epigenetic mechanism. Methods We cultured 3D PER2∷LUCIFERASE Bmal1-ELuciferase jejunal media supplemented with sterile...