Hepatocellular carcinoma (HCC) is the most common malignant liver tumor with poor clinical outcomes. Increasing amount of long non-coding RNAs (lncRNAs) have been revealed to be implicated in carcinogenesis and progression HCC. However, expressions, significances, roles lncRNAs HCC are still unknown. The expression lncRNA MCM3AP antisense RNA 1 (MCM3AP-AS1) tissues cell lines was detected by qRT-PCR fluorescence situ hybridization. Immunoblotting, CCK-8, EdU, colony formation flow cytometry...

Recently, it has been reported that long non-coding RNA (lncRNA) cancer susceptibility candidate 2 (CASC2), a novel tumor suppressor, participates in regulating the carcinogenesis and suppresses progression by sponging microRNAs (miRNAs). However, expression function of CASC2 hepatocellular carcinoma (HCC) remain unclear. The miR-367 HCC specimens cell lines were detected real-time PCR. Western blotting immunohistochemistry carried out for detection epithelial-to-mesenchymal transition (EMT)...

Heat shock protein 90 (HSP90) functions as a well-known onco-protein to regulate conformation, stability and degradation. Pyruvate kinase M2 (PKM2), critical regulator of the metabolism, growth metastasis cancer cells, has been confirmed be overexpressed in various human including hepatocellular carcinoma (HCC). However, molecular mechanisms underlying oncogenic HSP90 PKM2 overexpression HCC remain unknown.The expression specimens cells were detected by immunoblotting immunostaining. The...

Increasing evidences demonstrate that miRNAs contribute to development and progression of hepatocellular carcinoma (HCC). Underexpression miR-1296 is recently reported promote growth metastasis human cancers. However, the expression role in HCC remain unknown.The levels tissues cells were detected by qRT-PCR. Immunoblotting immunofluorescence used for detection epithelial-to-mesenchymal transition (EMT) cells. Transwell assays performed determine migration invasion A lung mouse model was...

Rational: Patients with hepatocellular carcinoma (HCC) have a poor prognosis mostly due to intrahepatic as well distal metastasis. Vasodilator-stimulated phosphoprotein (VASP), regulator of actin cytoskeleton and cell migration, is overexpressed in HCC correlated its malignant features prognosis. Very little known about function HCC. Methods: qRT-PCR, Western blot IHC were used detect the VASP expression tissues cells. Transwell wound healing assays measure migration invasion Immunoblotting...

// Zhikui Liu 1, * , Changwei Dou Bowen Yao 1 Meng Xu Linglong Ding Yufeng Wang Yuli Jia Qing Li Hongyong Zhang Kangsheng Tu Tao Song Qingguang Department of Hepatobiliary Surgery, The First Affiliated Hospital Xi’an Jiaotong University, Xi’an, Shaanxi, China These authors have contributed equally to this work Correspondence to: Liu, email: liuqingguang@vip.sina.com Song, 13572431619@163.com Tu, tks0912@foxmail.com Keywords: Ftx, miR-545, hepatocellular carcinoma, RIG-I,...

Accumulating evidence has highlighted the potential role of long non-coding RNAs (lncRNAs) in biological behaviors hepatocellular carcinoma (HCC). Here, we elucidated function and possible molecular mechanisms effect lncRNA-AGAP2-AS1 on HCC.EdU, Transwell flow cytometry were used to determine proliferation, migration, invasion apoptosis HCC cells vitro. The subcutaneous tumor model lung metastasis mouse nude mice was established detect growth vivo. direct binding miR-16-5p 3'UTR ANXA11...

Abstract Previous evidences reveal that long non-coding RNA (lncRNA) down syndrome critical region 8 (DSCR8) involves in the progression of multiple cancers. However, exact expression, function, and mechanism DSCR8 hepatocellular carcinoma (HCC) remain uncovered. In this study, real-time PCR HCC tissues cell lines indicated expression was upregulated, while miR-485-5p downregulated. MTT assay, plate clone formation, Edu flow cytometry, vivo experiments promoted proliferation cycle, whereas...

Cartilage oligomeric matrix protein (COMP) is known to promote fibrosis in skin, lung and liver. Emerging evidence shows that COMP plays critical roles tumor development, including breast cancer, colon cancer hepatocellular carcinoma (HCC). Nevertheless, the role of HCC proliferation metastasis its underlying mechanisms remain fully unclear. Serum was determined by ELISA. Cell Counting Kit-8 plate colony formation were performed evaluate cell proliferation. Wound healing transwell assays...

Abstract Background Accumulating evidence confirm that aberrant microRNAs (miRNAs) expression contributes to hepatocellular carcinoma (HCC) development and progression. Previous study reported miR-1468 showed an up-regulated tendency might be a potential prognostic biomarker in HCC samples derived from TCGA database. However, the role of its underlying mechanisms involved growth metastasis remain poorly investigated. Methods CCK-8, EdU, colony formation flow cytometry were used determine...

Cancer stem cells (CSCs) require stromal signals for maintaining pluripotency and self-renewal capacities to confer tumor metastasis. Resolvin D1 (RvD1), an endogenous anti-inflammatory lipid mediator, has recently been identified display anti-cancer effects by acting on stroma cells. Our previous study reveals that hepatic stellate (HSCs)-derived cartilage oligomeric matrix protein (COMP) contributes hepatocellular carcinoma (HCC) progression. However, whether RvD1 inhibits paracrine of...

Abstract Hepatocellular carcinoma (HCC) is characterised by a hypoxic microenvironment and high rate of heterogeneity recurrence, the presence cancer stem cells (CSCs) in HCC may well explain both these pathological properties. There mounting evidence that long non-coding RNAs (lncRNAs) participate carcinogenesis maintain stemness cells. However, expression modes, regulatory mechanisms potential roles stemness-related lncRNAs are still obscure. LncRNA RUNX1-IT1 intronic transcript 1 RUNX1,...

Rational: Increasing evidence indicates that the physical environment is a critical mediator of tumor behavior. Hepatocellular carcinoma (HCC) develops in an altered biomechanical environment, and increased matrix stiffness strong predictor HCC development. C-X-C chemokine receptor type 4 (CXCR4) known to trigger progression. However, CXCR4 as mechanical cues not well characterized. Methods: qRT-PCR, Western blot IHC were used detect expression different gels. MTT was measure cell...

Long non-coding RNAs (lncRNAs) are widely involved in human cancers' progression by regulating tumor cells' various malignant behaviors. MAPKAPK5-AS1 has been recognized as an oncogene colorectal cancer. However, the biological role of hepatocellular carcinoma (HCC) not explored.Quantitative real-time PCR was performed to detect level HCC tissues and cell lines. The effects on growth metastasis were assessed via vitro experiments, including MTT, colony formation, EdU, flow cytometry,...

Significance Hypoxia (reduced oxygen availability) is a common finding in the tumor microenvironment and plays critical role stimulating metastasis of breast cancer cells from primary to distant organs, which closely related patient mortality. Critical transcriptional responses reduced O 2 availability are mediated by hypoxia-inducible factors (HIFs). In this study, we demonstrate that hypoxia induces HIF-dependent expression disintegrin metalloproteinase 12 (ADAM12), clips off extracellular...

Hypoxic microenvironment, a common feature of hepatocellular carcinoma (HCC), can induce HIF-1α expression and promote the epithelial-mesenchymal transition (EMT) invasion cancer cells. However, underlying molecular mechanisms have not fully elucidated.HCC cells were cultured under controlled hypoxia conditions or normoxic conditions. Transwell assays used to examine migration capacity. siRNA, cyclopamine (a SMO antagonist) GLI1 siRNA inhibit transcription Hh signaling activation.In present...

// Changwei Dou 1 , Yufeng Wang Chao Li Zhikui Liu Yuli Jia Qing Wei Yang Yingmin Yao Qingguang and Kangsheng Tu Department of Hepatobiliary Surgery, The First Affiliated Hospital Xi’an Jiaotong University, Xi’an, China Correspondence to: Tu, email: Liu, Keywords : MiR-212, FOXA1, hepatocellular carcinoma, proliferation, apoptosis Received February 02, 2015 Accepted March 31, Published April 23, Abstract MicroRNA-212 (miR-212) has been reported to play oncogenic or tumor...

Histone citrullination by PADI4 is required for hypoxia-induced gene expression.

Abstract Hepatocellular carcinoma (HCC) is the most common type of liver cancer with poor clinical outcomes. Long non-coding RNAs (lncRNAs) are extensively involved in tumorigenesis and progression HCC. However, more investigations should be carried out on novel lncRNAs their effects Here we identified a lncRNA KDM4A-AS1, which was aberrantly overexpressed HCC tissues, associated unfavorable features prognosis patients. KDM4A-AS1 promoted cell proliferation, migration, invasion vitro...

Abstract Background Hepatocellular carcinoma (HCC) is the fourth fatal malignant tumour type worldwide. However, exact molecular mechanism involved in HCC progression remains unclear. Methods Three pairs of and matched portal vein thrombus (PVTT) tissue samples were analysed by isobaric tags for relative absolute quantification (iTRAQ) assay to investigate differentially expressed proteins. Real‐time quantitative PCR, immunostaining, immunoblotting performed detect cofilin 1 (CFL1)...

// Zhikui Liu 1, * , Changwei Dou Bowen Yao 1 Meng Xu Linglong Ding Yufeng Wang Yuli Jia Qing Li Hongyong Zhang Kangsheng Tu Tao Song Qingguang Department of Hepatobiliary Surgery, The First Affiliated Hospital Xi'an Jiaotong University, Xi'an, Shaanxi, China These authors contributed equally to this work Correspondence to: Tu, email: tks0912@foxmail.com Song, 13572431619@163.com Liu, liuqingguang@vip.sina.com Keywords: miR-129-2, methylation, high mobility group box invasion, hepatocellular...