A5017070659- Nicotinic Acetylcholine Receptors Study
- Clinical practice guidelines implementation
- Venomous Animal Envenomation and Studies
- Primary Care and Health Outcomes
- Ion channel regulation and function
- Renin-Angiotensin System Studies
- Healthcare cost, quality, practices
- Receptor Mechanisms and Signaling
- Health Policy Implementation Science
- Heart Failure Treatment and Management
- Electronic Health Records Systems
- Chronic Disease Management Strategies
- Chemical Synthesis and Analysis
- Atherosclerosis and Cardiovascular Diseases
- Artificial Intelligence in Healthcare
- Cardiovascular Disease and Adiposity
- Adipokines, Inflammation, and Metabolic Diseases
- Pulmonary Hypertension Research and Treatments
- Nitric Oxide and Endothelin Effects
- Insect and Pesticide Research
- Telemedicine and Telehealth Implementation
- Blood Pressure and Hypertension Studies
- Health Systems, Economic Evaluations, Quality of Life
- Patient-Provider Communication in Healthcare
- Cardiovascular Function and Risk Factors
Western Health
2019-2024
The University of Melbourne
2020-2024
Baker Heart and Diabetes Institute
2010-2021
Government of Western Australia Department of Health
2021
Sunshine Hospital
2020
Barts Health NHS Trust
2014
The University of Queensland
2006-2009
Monash University
2003-2005
The endothelium plays a fundamental role in maintaining vascular homeostasis by releasing factors that regulate local blood flow, systemic pressure, and the reactivity of leukocytes platelets. Accordingly, endothelial dysfunction underpins many cardiovascular diseases, including hypertension, myocardial infarction, stroke. Herein, we evaluated mice with endothelial-specific deletion Nppc, which encodes C-type natriuretic peptide (CNP), determined this mediator is essential for multiple...
We report the discovery of conopressin-T, a novel bioactive peptide isolated from Conus tulipa venom. Conopressin-T belongs to vasopressin-like family and displays high sequence homology mammalian hormone oxytocin (OT) vasotocin, endogenous vasopressin analogue found in teleost fish, cone snail's prey. was act as selective antagonist at human V1a receptor. All peptides this contain two conserved amino acids within exocyclic tripeptide (Pro7 Gly9), which are replaced with Leu7 Val9...
Angiotensin AT2 receptors are upregulated in disease states such as atherosclerosis and blockade of the exacerbates plaque formation. Direct stimulation these is anti-atherogenic but mechanisms pathways involved remain unknown. We examined effect direct receptor with Compound 21 (C21) on leukocyte adhesion cascade vitro, right through to formation vivo.Effects C21 TNFα-induced inflammation were assessed human umbilical vein endothelial cells (HUVECs), activation monocytes, polarisation...
Pre-clinical studies have identified nitroxyl (HNO), the reduced congener of nitric oxide (NO•), as a potent vasodilator which is resistant to tolerance development. The present study explores efficacy HNO in human blood vessels and describes, for first time, humans that not susceptible tolerance. Human radial arteries saphenous veins were obtained from patients undergoing coronary artery graft surgery mounted organ baths. Repeated responses donor Angeli's salt (AS) NO• glyceryl trinitrate...
Cone snail venom is a rich source of bioactives, in particular small disulfide peptides that disrupt synaptic transmission. Here, we report the discovery conomap‐Vt (Conp‐Vt), an unusual linear tetradecapeptide isolated from Conus vitulinus venom. The sequence displays no homology to known conopeptides, but significant MATP (myoactive tetradecapeptide) family, which are important endogenous neuromodulators molluscs, annelids and insects. Conp‐Vt showed potent excitatory activity several...
Abstract Background Early identification and treatment of chronic disease is associated with better clinical outcomes, lower costs, reduced hospitalisation. Primary care ideally placed to identify patients at risk of, or in the early stages implement prevention intervention measures. This paper evaluates implementation a technological called Future Health Today that integrates general practice EMRs (1) at-risk undiagnosed untreated, kidney (CKD), (2) provide guideline concordant...
Soluble P-selectin (sP-selectin), a biomarker of inflammatory related pathologies including cardiovascular and peripheral vascular diseases, also has pro-atherosclerotic effects the ability to increase leukocyte recruitment modulate thrombotic responses in vivo. The current study explores its role progressing atherosclerotic plaque disease. Apoe-/- mice placed on high fat diet (HFD) were given daily injections recombinant dimeric murine (22.5 µg/kg/day) for 8 or 16 weeks. Saline sE-selectin...
Objective To codesign an electronic chronic disease quality improvement tool for use in general practice. Design Service design employing strategies. Setting General Participants Seventeen staff (general practitioners, nurses and practice managers) from metropolitan Melbourne regional Victoria five patients Melbourne. Interventions Codesign sessions with staff, using a service approach, were conducted to explore key criteria functionality of the audit feedback clinical decision support...
Nitroxyl anion (HNO) donors are currently being assessed for their therapeutic utility in several cardiovascular disorders including heart failure. Here, we examine effect on factors that precede atherosclerosis endothelial cell and monocyte activation, leucocyte adhesion to the endothelium macrophage polarization. Similar NO donor glyceryl trinitrate (GTN), HNO Angeli's salt (AS) isopropylamine NONOate (IPA/NO) decreased activated human umbilical vein cells (HUVECs) mouse isolated aorta....
Summary 1 The great taxonomic and prey base diversity of colubrids (non‐front‐fanged snakes) suggests that their venoms may represent a ‘literal gold mine’ for scientists eager to find novel pharmacological probes ( Mackessy, 2002 ). 2 While characterization is lacking most these venoms, this even more so with regard activity colubrid on the mammalian autonomic nervous system. This study characterizes venom from colubrid, Boiga dendrophila using in vitro smooth muscle preparations...
This single visit study examined whether endothelial function, in addition to cardiovascular (CV) risk factors and plasma microparticle content, was normalised 15 patients with type 2 diabetes + acute coronary syndrome (ACS) (6 weeks–6 months post cardiac event) undergoing standard clinical care compared 16 sex- age-matched healthy controls. Results: While total low-density lipoprotein (LDL) cholesterol levels were well controlled the ACS, residual CV profiles such as increased body mass...
Primary care plays a key role in the management of type 2 diabetes. Sodium-glucose cotransporter (SGLT2) inhibitors have been demonstrated to reduce hospitalization and cardiac renal complications. Tools that optimize management, including appropriate prescribing, are priority for treating chronic diseases. Future Health Today (FHT) is software facilitates clinical decision support quality improvement. FHT applies algorithms data stored electronic medical records general practice identify...
Interrelated chronic vascular diseases (chronic kidney disease (CKD), type 2 diabetes (T2D) and cardiovascular (CVD)) are common with high morbidity mortality. This study aimed to assess if an electronic-technology-based quality improvement intervention in primary care could improve detection management of people at risk these diseases.Stepped-wedge trial practices randomised commence one five 16-week periods. Intervention included (1) electronic-technology tool extracting data from general...
Abstract The aetiology and progression of hypertension involves various endogenous systems, such as the renin angiotensin system, sympathetic nervous endothelial dysfunction. Recent data suggest that vascular inflammation may also play a key role in pathogenesis hypertension. This study sought to determine whether high intraluminal pressure results inflammation. Leukocyte adhesion was assessed rat carotid arteries exposed 1 h pressure. effect on signaling mechanisms including reactive oxygen...
Objectives To evaluate the capacity of general practice (GP) electronic medical record (EMR) data to assess risk factor detection, disease diagnostic testing, diagnosis, monitoring and pharmacotherapy for interrelated chronic vascular diseases—chronic kidney (CKD), type 2 diabetes (T2D) cardiovascular disease. Design Cross-sectional analysis extracted on a single date each between 12 April 2017 18 incorporating from any time or before extraction, using baseline Chronic Disease early...
A simple and efficient method has been developed for the synthesis of two anthranilamide-based non-peptide mimetics ω-conotoxin GVIA. These anthranilamide derivatives aim to mimic K2, R17, Y13 residues peptide. The synthetic route described enables rapid analogues with identical alkyl chain lengths. target compounds show affinity rat N-type voltage gated calcium channels (Cav2.2) EC50 values 42 75 μM.
Worldwide, Chronic Kidney Disease (CKD), directly or indirectly, causes more than 2.4 million deaths annually with symptoms generally presenting late in the disease course. Clinical guidelines support early identification and treatment of CKD to delay progression improve clinical outcomes. This paper reports protocol for codesign, implementation evaluation a technological platform called Future Health Today (FHT), software program that aims optimise detection management general practice. FHT...