Normal activation of platelets and their aggregation are crucial for proper hemostasis. It appears that excessive or abnormal may bring about cardiovascular diseases such as stroke, atherosclerosis, thrombosis. For this reason, finding a substance can regulate platelet suppress will aid in the prevention treatment diseases. Artesunate is compound extracted from plant roots

Glycoprotein IIb/IIIa (αIIb/β

The ability to inhibit aggregation has been demonstrated with synthetically derived ginsenoside compounds G-Rp (1, 3, and 4) ginsenosides naturally found in Panax ginseng 20(S)-Rg3, Rg6, F4, Ro. Among these compounds, Rk3 (G-Rk3) from needs be further explored order reveal the mechanisms of action during inhibition.Our study focused investigate G-Rk3 on agonist-stimulated human platelet aggregation, inhibition signaling molecules such as fibrinogen binding integrin αIIbβ3 using flow...

Thromboxane A

The root of Panax ginseng is used in ethnomedicine throughout eastern Asia and various recent studies have proved that has inhibitory effects on cardiovascular disease. Each factor causing disease known to a very complex process which achieved by diverse number mechanisms. Among these factors, platelets are the most important because they directly participate thrombogenesis. Therefore, inhibiting activity an essential element for prevention diseases. Our previous study showed antiplatelet...

Synthetic ginsenoside compounds G-Rp (1,3, and 4) natural ginsenosides in Panax ginseng 20(S)-Rg3, Rg6, F4 Ro have inhibitory actions on human platelets. However, the mechanism of Rk1 (G-Rk1) is still unclear thus, we initiated investigation anti-platelet by G-Rk1 from ginseng. Our study focused to investigate action agonist-stimulated platelet aggregation, inhibition signaling molecules such as fibrinogen binding with integrin αIIbβ3 using flow cytometry, intracellular calcium mobilization,...

Panax ginseng (P. ginseng), one of the most valuable medicinal plants, is known for its healing and immunobooster properties has been widely used in folk medicine against cardiovascular diseases, including stroke heart attack. In this study, we explored anti-platelet activity gintonin (a recently discovered non-saponin fraction ginseng) agonist-induced platelet activation. vitro effects on human rat aggregation, granule secretion, integrin αIIbβ3 activation, intracellular calcium ion...

Binding of adhesive proteins (i.e., fibrinogen, fibronectin, vitronectin) to platelet integrin glycoprotein IIb/IIIa (αIIb/β3) by various agonists (thrombin, collagen, adenosine diphosphate) involve in strength thrombus. This study was carried out evaluate the antiplatelet effect total saponin from Korean Red Ginseng (KRG-TS) investigating whether KRG-TS inhibits thrombin-induced binding fibrinogen and fibronectin αIIb/β3. We investigated on phosphorylation vasodilator-stimulated...

The prevalence of cardiovascular diseases (CVDs) is increasing at a high rate, and the available treatment options, sometimes, have complications which necessitates need to develop safer efficacious approaches. Ethnomedicinal applications reportedly reduce CVD risk. Ulmus parvifolia Jacq. (Ulmaceae) commonly known as Chinese Elm or Lacebark Elm, native China, Japan, Korea. It exhibits anti-inflammatory, antiviral, anticancer properties, but its anti-platelet properties not yet been...

Intracellular Ca(2+) ([Ca(2+)] i ) is platelet aggregation-inducing molecule and involved in activation of aggregation associated molecules. This study was carried out to understand the Ca(2+)-antagonistic effect ginsenoside Ro (G-Ro), an oleanane-type saponin Panax ginseng. G-Ro, without affecting leakage lactate dehydrogenase, dose-dependently inhibited thrombin-induced aggregation, half maximal inhibitory concentration approximately 155 μM. G-Ro strongly thrombin-elevated [Ca(2+)] , which...

A species of the fungal genus Cordyceps has been used as a complementary and alternative medicine traditional Chinese medicine, its major component cordycepin cordycepin-enriched WIB-801CE are known to have antiplatelet effects in vitro. However, it is unknown whether they also endogenous antithrombotic effects. In this study, resolve these doubts, we prepared WIB-801CE, an ethanol extract from militaris-hypha, then evaluated ex vivo, vitro Ex vivo on collagen- ADP-induced platelet...

Intracellular Ca2+([Ca2+]i) is a platelet aggregation-inducing molecule. Therefore, understanding the inhibitory mechanism of [Ca2+]i mobilization very important to evaluate antiplatelet effect substance. This study was carried out understand Ca2+-antagonistic total saponin from Korean Red Ginseng (KRG-TS). We investigated KRG-TS on cyclic nucleotides-associated phosphorylation inositol 1,4,5-trisphosphate receptor type I (IP3RI) and adenosine monophosphate (cAMP)-dependent protein kinase...

Platelets play a very significant role in hemostasis while simultaneously posing risk for the development of various cardiovascular diseases. Platelet-mediated issues can occur blood vessels and trigger medical problems. Therefore, controlling platelet function is important prevention thrombosis. In this regard, we need to find compounds that provide potent antiplatelet activity with minimum side effects. examined effect 5-hydroxyindolin-2-one isolated from Protaetia brevitarsis larvae...

Abstract Cudrania tricuspidata ( C. ) is widespread throughout Asia and has known to have various physiological activities such as, inflammation, diabetes, obesity tumor. , a rich source of xanthones flavonoids, been investigated phytochemically biologically. However, research these compounds on platelets limited. Therefore, we searched for new substance from flavonoids in . We confirmed the results that steppogenin isoderrone suppress human among components isolated as result analyzing...

Isoderrone은 Cudrania tricuspidata의 성분 중 하나로 혈소판에서의 억제기작에 대한 연구는 아직 보고되지 않았다. 본 연구에서는 isoderrone이 platelet aggregation에 미치는 영향을 평가하였다. 그 결과 isoderrone은 콜라겐이 유도한 aggregation을 강력하게 억제하였고, 세포 내 CaSUP2+/SUP 동원과 αⅡb/β3의 활성을 감소시켰다. 이 결과들은 이와 관련된 신호전달 인자인 IP3RI와 VASP의 인산화 조절을 통해서 발생한다는 것을 규명하였다. 따라서 인체 혈소판에서 억제하는 천연물질로서 잠재적 가치가 있다고 여겨진다.

Cudrania tricuspidata (C. tricuspidata) is a deciduous tree found in Japan, China and Korea. The root, stems, bark fruit of C. has been used as traditional herbal remedies such eczema, mumps, acute arthritis tuberculosis. In this study, we investigated the potential efficacies natural compound by focusing on inhibitory effect cudraxanthone L (CXL) isolated from roots human platelet aggregation. Our study focused action CXL collagen-stimulated aggregation, inhibition signaling molecules...

Physiological agonists trigger signaling cascades, called “inside-out signaling”, and activated platelets facilitate adhesion, shape change, granule release, structural change of glycoprotein IIb/IIIa (αIIb/β3). Activated αIIb/β3 interacts with fibrinogen begins second cascades “outside-in signaling”. These two pathways can lead to hemostasis or thrombosis. Thrombosis occur in arterial venous blood vessels is a major medical problem. Platelet-mediated thrombosis cause cardiovascular disease...

Platelets play fundamental roles in hemostasis and thrombosis, antiplatelet drugs effectively reduce thrombosis patients with cardiovascular disease.However, various side effects have been reported, although developed, mortality rates of diseases not decreased.Therefore, new fewer are urgently required.This study examined whether Tenebrio molitor extract inhibits platelet aggregation by regulating integrin αIIb/β3 its associated signaling molecules.Tenebrio inhibited activation...