Aim: In this study, we investigated the effect of (−)-epigallocatechin-3-gallate (EGCG) on cyclic nucleotide production and vasodilator-stimulated phosphoprotein (VASP) phosphorylation in collagen (10 µg/mL)-stimulated platelet aggregation.Methods: Washed platelets (108/mL) from Sprague-Dawley rats (6-7 weeks old, male) were preincubated for 3 min at 37°C presence 2 mM exogenous CaCl2 with or without EGCG other materials, stimulated µg/mL) 5 min, then used determination intracellular...

Normal activation of platelets and their aggregation are crucial for proper hemostasis. It appears that excessive or abnormal may bring about cardiovascular diseases such as stroke, atherosclerosis, thrombosis. For this reason, finding a substance can regulate platelet suppress will aid in the prevention treatment diseases. Artesunate is compound extracted from plant roots

Glycoprotein IIb/IIIa (αIIb/β

The ability to inhibit aggregation has been demonstrated with synthetically derived ginsenoside compounds G-Rp (1, 3, and 4) ginsenosides naturally found in Panax ginseng 20(S)-Rg3, Rg6, F4, Ro. Among these compounds, Rk3 (G-Rk3) from needs be further explored order reveal the mechanisms of action during inhibition.Our study focused investigate G-Rk3 on agonist-stimulated human platelet aggregation, inhibition signaling molecules such as fibrinogen binding integrin αIIbβ3 using flow...

In this study, we investigated the effect of chlorogenic acid, a phenolic on collagen (10 μg/ml)-stimulated platelet aggregation. Chlorogenic acid dose-dependently inhibited collagen-induced aggregation, and suppressed production thromboxane A2 (TXA2), an intracellular Ca-agonist as aggregation-inducing autacoidal molecule, which was associated with strong inhibition cyclooxygenase (COX)-1 in microsomes having cytochrome c reductase activity. addition, increased significantly formation...

Thromboxane A

The root of Panax ginseng is used in ethnomedicine throughout eastern Asia and various recent studies have proved that has inhibitory effects on cardiovascular disease. Each factor causing disease known to a very complex process which achieved by diverse number mechanisms. Among these factors, platelets are the most important because they directly participate thrombogenesis. Therefore, inhibiting activity an essential element for prevention diseases. Our previous study showed antiplatelet...

Synthetic ginsenoside compounds G-Rp (1,3, and 4) natural ginsenosides in Panax ginseng 20(S)-Rg3, Rg6, F4 Ro have inhibitory actions on human platelets. However, the mechanism of Rk1 (G-Rk1) is still unclear thus, we initiated investigation anti-platelet by G-Rk1 from ginseng. Our study focused to investigate action agonist-stimulated platelet aggregation, inhibition signaling molecules such as fibrinogen binding with integrin αIIbβ3 using flow cytometry, intracellular calcium mobilization,...

Panax ginseng (P. ginseng), one of the most valuable medicinal plants, is known for its healing and immunobooster properties has been widely used in folk medicine against cardiovascular diseases, including stroke heart attack. In this study, we explored anti-platelet activity gintonin (a recently discovered non-saponin fraction ginseng) agonist-induced platelet activation. vitro effects on human rat aggregation, granule secretion, integrin αIIbβ3 activation, intracellular calcium ion...

Binding of adhesive proteins (i.e., fibrinogen, fibronectin, vitronectin) to platelet integrin glycoprotein IIb/IIIa (αIIb/β3) by various agonists (thrombin, collagen, adenosine diphosphate) involve in strength thrombus. This study was carried out evaluate the antiplatelet effect total saponin from Korean Red Ginseng (KRG-TS) investigating whether KRG-TS inhibits thrombin-induced binding fibrinogen and fibronectin αIIb/β3. We investigated on phosphorylation vasodilator-stimulated...

The prevalence of cardiovascular diseases (CVDs) is increasing at a high rate, and the available treatment options, sometimes, have complications which necessitates need to develop safer efficacious approaches. Ethnomedicinal applications reportedly reduce CVD risk. Ulmus parvifolia Jacq. (Ulmaceae) commonly known as Chinese Elm or Lacebark Elm, native China, Japan, Korea. It exhibits anti-inflammatory, antiviral, anticancer properties, but its anti-platelet properties not yet been...

Abstract Here we report inhibition of phospholipase C‐β1 (PLC‐β1)‐mediated signaling by post‐translational glycosylation with β‐ N ‐acetylglucosamine ( O ‐GlcNAc modification). In C2C12 myoblasts, isoform‐specific knock‐down experiments using siRNA showed that activation bradykinin (BK) receptor led to stimulation PLC‐β1 and subsequent intracellular Ca 2+ mobilization. myotubes, modification was markedly enhanced in response treatment glucosamine (GlcNH 2 ), an inhibitor ‐GlcNAase (PUGNAc)...

Intracellular Ca(2+) ([Ca(2+)] i ) is platelet aggregation-inducing molecule and involved in activation of aggregation associated molecules. This study was carried out to understand the Ca(2+)-antagonistic effect ginsenoside Ro (G-Ro), an oleanane-type saponin Panax ginseng. G-Ro, without affecting leakage lactate dehydrogenase, dose-dependently inhibited thrombin-induced aggregation, half maximal inhibitory concentration approximately 155 μM. G-Ro strongly thrombin-elevated [Ca(2+)] , which...

A species of the fungal genus Cordyceps has been used as a complementary and alternative medicine traditional Chinese medicine, its major component cordycepin cordycepin-enriched WIB-801CE are known to have antiplatelet effects in vitro. However, it is unknown whether they also endogenous antithrombotic effects. In this study, resolve these doubts, we prepared WIB-801CE, an ethanol extract from militaris-hypha, then evaluated ex vivo, vitro Ex vivo on collagen- ADP-induced platelet...

Intracellular Ca2+([Ca2+]i) is a platelet aggregation-inducing molecule. Therefore, understanding the inhibitory mechanism of [Ca2+]i mobilization very important to evaluate antiplatelet effect substance. This study was carried out understand Ca2+-antagonistic total saponin from Korean Red Ginseng (KRG-TS). We investigated KRG-TS on cyclic nucleotides-associated phosphorylation inositol 1,4,5-trisphosphate receptor type I (IP3RI) and adenosine monophosphate (cAMP)-dependent protein kinase...

Intracellular Ca2+ ([Ca2+]i) induces platelet aggregation, and influences the activation of aggregation associated-molecules. The increased [Ca2+]i activates both Ca2+/calmodulin-dependent phosphorylation myosin light chain diacylglycerol-dependent pleckstrin to trigger granule secretion (i.e., dense body α-granule) aggregation. This study was carried out elucidate antagonistic effect 20(S)-ginsenoside Rg3 (G-Rg3) present in Panax ginseng Mayer on Ca2+. G-Rg3 inhibited thrombin-induced human...

Seung Na Ko, Ji Won Son, Gyu Ri Kim, Min Seon Yea Jin Lee, Ju Hyeon Shin, Da In Jo, Woo Young Bok, Hye Gyo Oh and Hyuk-Woo Kwon. BSL 2023;29:242-8. https://doi.org/10.15616/BSL.2023.29.4.242

Pest control in the agricultural fields, a major concern globally, is currently achieved through chemical or biological methods. Chemical methods, which leave toxic residue produce, are less preferred than Venoms injected by stings of various wasps that kill pest considered as examples method. Although several studies have investigated pests these venoms, very few reported effects venoms on mammalian cells. Bracon hebetor, an ectoparasitoid order Hymenoptera, having paramount importance...

Platelets are activated at sites of vascular injury via several molecules, such as adenosine diphosphate, collagen and thrombin. Full platelet aggregation is absolutely essential for normal hemostasis. Moreover, this physiological event can trigger circulatory disorders, thrombosis, atherosclerosis, cardiovascular disease. Therefore, function inhibition a promising approach in preventing platelet-mediated Many studies reported the involvement mitogen-activated protein kinases (MAPKs)...

Glycoprotein IIb/IIIa (αIIb/β3) is the most abundant integrin on platelet surfaces, which involved in interaction between platelets, and triggers an intracellular signaling cascade, shape changes, granule secretion, clot retraction. In this study, we evaluated effect of ginsenoside Ro (G-Ro) binding fibronectin fibrinogen to αIIb/β3 We found that G-Ro inhibited thrombin-induced aggregation dose-dependently attenuated fibronectin-, fibrinogen-binding through dephosphorylation phosphoinositide...

The Panax ginseng Mayer is used in conventional medicine Asia owing to its preventing effects on thrombosis, hypertension, atherosclerosis, vasorelaxation and myocardial infarction. Because platelets are crucial mediators of cardiovascular diseases, many studies have investigated functions. previous study showed the antiplatelet crude fraction two components, ginsenoside Rg3 (20S 20R). In addition, Rg3-enriched shows an inhibitory effect collagen-activated rat platelets. However, mechanism...