A5100714542- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Ion channel regulation and function
- NMR spectroscopy and applications
- Protein Structure and Dynamics
- Solid-state spectroscopy and crystallography
- Electron Spin Resonance Studies
- Catalytic C–H Functionalization Methods
- Mass Spectrometry Techniques and Applications
- Mesoporous Materials and Catalysis
- Cardiac electrophysiology and arrhythmias
- Advancements in Battery Materials
- Mitochondrial Function and Pathology
- Blood disorders and treatments
- Zeolite Catalysis and Synthesis
- Lipid Membrane Structure and Behavior
- Radical Photochemical Reactions
- Nuclear Physics and Applications
- Parkinson's Disease Mechanisms and Treatments
- Synthesis and Catalytic Reactions
- Muon and positron interactions and applications
- Cardiovascular Function and Risk Factors
- Sulfur-Based Synthesis Techniques
- Cardiomyopathy and Myosin Studies
- Chemical Synthesis and Analysis
University of Wisconsin–Madison
2023-2025
Resonance Research (United States)
2025
Henan Institute of Science and Technology
2016-2024
University of Minnesota
2017-2024
North China University of Water Resources and Electric Power
2022-2024
Henan Normal University
2023
University of Illinois Chicago
2012-2021
Twin Cities Orthopedics
2020
University of Hong Kong
2019
Capital Medical University
2015
Amyloid-β (Aβ) fibrils in neuritic plaques are a hallmark of Alzheimer's disease (AD). Since the 42-residue Aβ (Aβ42) fibril is most pathogenic among different species, its structural characterization crucial to our understanding AD. While several polymorphs have been reported for Aβ40, previous studies Aβ42 prepared at neutral pH detected essentially only one structure, with an S-shaped β-sheet arrangement (e.g., Xiao et al. Nat. Struct. Mol. Biol. 2015, 22, 499). Herein, we demonstrate...
Abstract EmrE is a bacterial membrane-embedded multidrug transporter that functions as an asymmetric homodimer. implicated in antibiotic resistance, but now known to confer either resistance or susceptibility depending on the identity of small molecule substrate. Here, we report both solution- and solid-state NMR assignments S64V-EmrE at pH 5.8, below pKa critical residues E14 H110. This includes 1 H, 15 N, 13 C resonance backbone, methyl groups (isoleucine, leucine, valine, threonine...
1H-detected magic-angle spinning (MAS) NMR experiments have revolutionized the studies of biological and inorganic solids by providing unparalleled sensitivity resolution. Despite these gains, homogeneous broadening, originating from incomplete removal homonuclear dipolar interactions under fast MAS, remains highly prevalent limits achievable In direct analogy to super-resolution microscopy methods, we show that resolution beyond currently MAS alone can be obtained experiment-driven...
We present a 3D <sup>1</sup>H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10–100 nmol fully protonated proteins.
Phospholamban (PLN) is a mini-membrane protein that directly controls the cardiac Ca
The synthesis of ZSM-5 microparticles (MPs) possesses scientific and industrial importance due to the corresponding kinetic effects operation issues. In this paper, a simple scalable crystal seed growth approach was developed synthesize MPs, without an organic template, for catalytic hydration cyclohexene cyclohexanol. As sub-microparticles (SMPs) were used as seeds, as-synthesized MPs exhibit higher crystalline degrees larger sizes, with average diameter up 8.78 μm, than that commercial...
We present a general approach in 1H-detected 13C solid-state NMR (SSNMR) for side-chain signal assignments of 10-50 nmol quantities proteins using combination high magnetic field, ultra-fast magic-angle spinning (MAS) at ~80 kHz, and stereo-array-isotope-labeled (SAIL) [Kainosho M. et al., Nature 440, 52–57, 2006]. First, we demonstrate that 1H indirect detection improves the sensitivity resolution SSNMR SAIL MAS condition. was performed micro-crystalline ubiquitin (~55 or ~0.5mg)...
A small peptide regulates muscle thermogenesis through interaction with the sarcoplasmic reticulum calcium ATPase.
We describe Cu-catalyzed intermolecular alkynylation and allylation of unactivated C(sp3)-H bonds with singly occupied molecular orbital-philes (SOMO-philes) via hydrogen atom transfer (HAT). Employing N-fluoro-sulfonamide as a HAT reagent, set substituted alkene alkyne compounds were synthesized in high yields good regioselectivity functional-group compatibility. Late-stage functionalization natural products drug molecules is also demonstrated.
γ-polyglutamic acid (γ-PGA), as an environmentally sustainable material, is extensive applied in agriculture for enhancing water and fertilizer utilization efficiency, augmenting crop yield, ameliorating soil conditions. However, the effect of γ-PGA conjunction with sesame cake on environment remains uncertain.The aim this study to investigate nutrients, use efficiency (WUE) nitrogen (NUE), maize yield across various levels fertilizer. Additionally, seeks identify optimal ratio establish a...
Here we report a metal‐ and light‐free decarboxylative functionalization approach enabled by reduced nicotinamide adenine dinucleotide (NADH) analogues. The efficient operationally simple approach, conducted in 5 minutes from in‐situ preparation of aryliodine (III) dicarboxylates under open‐air ambient conditions, enables diverse bond formation exhibits broad substrate scope over 70 examples. Late‐stage drug molecules natural products further demonstrates the synthetic utility this method....
Solid-phase peptide synthesis (SPPS) is a widely used technique in biology and chemistry. However, the yield SPPS often drops drastically for longer amino acid sequences, presumably because of occurrence incomplete coupling reactions. The underlying cause this problem hypothesized to be sequence-dependent propensity form secondary structures through protein aggregation. few methods are available study site-specific structure proteins or long peptides that anchored solid support SPPS. This...