Introduction: Chronic pancreatitis (CP) can lead to malnutrition, an established risk factor for low bone mineral density (BMD) and fractures. This study aims determine the prevalence of BMD, assess fracture incidence explore factors fractures in patients with CP. Patients methods: We performed a retrospective analysis all treated CP at Karolinska University Hospital between January 1999 December 2020. Electronic medical records were retrieved demographic, laboratory clinical data. subjected...

Introduction: Chronic pancreatitis (CP) is a long-standing progressive inflammation of the pancreas, which can lead to variety vascular complications, such as splanchnic venous thrombosis (VT) and arterial pseudoaneurysm (PA). There lack studies on complications in Scandinavian countries. Methods: We performed retrospective analysis medical records patients with CP identified from Karolinska University Hospital database between 2003 2018. A total 394 definite were included study. Results: 33...

Abstract In pancreatic ductal adenocarcinoma, the infiltration of CD8+ T cells within tumor microenvironment correlates with a favorable prognosis. However, significant proportion tumor-infiltrating become trapped desmoplastic stroma and lack reactivity. Here, we explored different T-cell subsets in tumors adjacent tissues. We identified subset cells, double positive (DP) for CD39 CD103 tumors, which has recently been described to display reactivity other types solid tumors. Interestingly,...

Background Paraduodenal pancreatitis (PDP) is a particular form of chronic (CP) occurring in and around the duodenal wall. Despite its low prevalence, this rare condition presents significant challenge clinical practice.Methods We retrospectively analysed electronic medical charts all patients with diagnosis identified those PDP, between January 1999 December 2020.Results There were 35 diagnosed PDP (86% males 14% females); median age 56 ± 11 (range 38–80). Alcohol overconsumption was...

Introduction Although abdominal pain is the most prevalent and disabling symptom in patients with chronic pancreatitis (CP), there are also who have painless CP.Patients methods We performed a retrospective analysis of diagnosis CP. A total 279 definite CP completed demographic clinical data were included final analysis.Results There 75 (26.9%) These had significantly higher mean age at diagnosis, 61.7 years, than 52.5 years (p < 0.001). Painless painful similar rates diabetes mellitus (DM)...

&lt;p&gt;Proportion of T cells co-expressing PD-1 and LAG-3.&lt;/p&gt;

&lt;p&gt;Patient characteristics&lt;/p&gt;

&lt;p&gt;Proportion of tumor/stroma area and immune cells in pancreatic tissues.&lt;/p&gt;

&lt;p&gt;Overall survival curves for DP CD8 T cells and SPCD39, CD4 cells.&lt;/p&gt;

&lt;p&gt;Proportion of immune cells in pancreatic tissues. The proportion different subsets were studied by flow cytometry central tissues (CT; blue), peripheral (PT; purple), non-tumor (NTT; brown), and patients’ PBMCs (black). &lt;b&gt;A,&lt;/b&gt; Tumor reactive stroma area calculated QuPath the three tissue compartments (&lt;i&gt;n&lt;/i&gt; = 9). &lt;b&gt;B,&lt;/b&gt; Total numbers CD4&lt;sup&gt;+&lt;/sup&gt; CD8&lt;sup&gt;+&lt;/sup&gt; T per mg 40). &lt;b&gt;C,&lt;/b&gt; Frequency from...

&lt;p&gt;Effects of presurgical chemotherapy on the immune landscape. The landscape for patients that underwent upfront surgery (gray squares) was compared with neoadjuvant followed by (white squares). &lt;b&gt;A,&lt;/b&gt; Kaplan–Meier survival curves comparing two groups. &lt;b&gt;B,&lt;/b&gt; Proportion CD39&lt;sup&gt;+&lt;/sup&gt; CD103&lt;sup&gt;+&lt;/sup&gt; CD8&lt;sup&gt;+&lt;/sup&gt; T cells. &lt;b&gt;C,&lt;/b&gt; Left, CD4&lt;sup&gt;+&lt;/sup&gt; and cells out...

&lt;div&gt;Abstract&lt;p&gt;In pancreatic ductal adenocarcinoma, the infiltration of CD8&lt;sup&gt;+&lt;/sup&gt; T cells within tumor microenvironment correlates with a favorable prognosis. However, significant proportion tumor-infiltrating become trapped desmoplastic stroma and lack reactivity. Here, we explored different T-cell subsets in tumors adjacent tissues. We identified subset cells, double positive (DP) for CD39 CD103 tumors, which has recently been described to display reactivity...

&lt;p&gt;T regulatory cells out of CD4+ and CD39+ T cells.&lt;/p&gt;

&lt;p&gt;Proportion of tumor/stroma area and immune cells in pancreatic tissues.&lt;/p&gt;

&lt;p&gt;Expression of coinhibitory markers on T cells expressing chemokine receptors. &lt;b&gt;A,&lt;/b&gt; Representative flow cytometry zebra plots CD8&lt;sup&gt;+&lt;/sup&gt; showing the gating strategy to identify CCR5, CXCR3, CXCR4, CXCR5, and CXCR6 positive cells. &lt;b&gt;B,&lt;/b&gt; Proportion CCR5 (&lt;i&gt;n&lt;/i&gt; = 12), CXCR3 15), CXCR4 14), CXCR5 6) CD4&lt;sup&gt;+&lt;/sup&gt; from central peripheral tumor tissues, non-tumor tissues patient PBMCs. &lt;b&gt;C,&lt;/b&gt;...

&lt;p&gt;Characterization of CD39&lt;sup&gt;+&lt;/sup&gt;CD103&lt;sup&gt;+&lt;/sup&gt; CD8&lt;sup&gt;+&lt;/sup&gt; T cells. &lt;b&gt;A,&lt;/b&gt; Representative flow cytometry histograms showing the data in B. &lt;b&gt;B,&lt;/b&gt; Proportion CD39&lt;sup&gt;−&lt;/sup&gt; CD103&lt;sup&gt;+&lt;/sup&gt; (SP CD103), CD39&lt;sup&gt;+&lt;/sup&gt; (DP), and CD103&lt;sup&gt;−&lt;/sup&gt; (DN) cells expressing PD-1 (&lt;i&gt;n&lt;/i&gt; = 36), TIM-3 PD-1-TIM-3 Ki67 6), TCF1 6) central peripheral...

&lt;p&gt;Tumor-infiltrating CD39&lt;sup&gt;+&lt;/sup&gt;CD103&lt;sup&gt;+&lt;/sup&gt; T cells. &lt;b&gt;A,&lt;/b&gt; A t-SNE analysis showing cluster point plots (&lt;a href="#bib1 bib2 bib3 bib4 bib5 bib6 bib7 bib8" target="_blank"&gt;1–8&lt;/a&gt;) and sample distribution in central (&lt;i&gt;n&lt;/i&gt; = 5) peripheral tumor tissues on 10-parameters flow cytometry analyses. &lt;b&gt;B,&lt;/b&gt; Distribution of each parameter as a single cell level. The color shows the median fluorescence...

&lt;p&gt;Coinhibitory receptor expression on CD4&lt;sup&gt;+&lt;/sup&gt; and CD8&lt;sup&gt;+&lt;/sup&gt; tumor-infiltrating T cells. Proportion of (&lt;b&gt;A&lt;/b&gt;) (&lt;b&gt;B&lt;/b&gt;) cells expressing PD-1 (CT &lt;i&gt;n&lt;/i&gt; = 54, PT 52, NTT 42), TIM-3 53, 51, 41), LAG-3 (NTT 6, CT 16, 16), CTLA-4 9, 8,NTT 2), coexpressing coinhibitory markers 41; &lt;b&gt;C,&lt;/b&gt; left), flow cytometry representative dot plots (right) to identify coexpression TIM-3. &lt;b&gt;D,&lt;/b&gt;...

&lt;p&gt;Tumor-infiltrating CD39&lt;sup&gt;+&lt;/sup&gt;CD103&lt;sup&gt;+&lt;/sup&gt; T cells. &lt;b&gt;A,&lt;/b&gt; A t-SNE analysis showing cluster point plots (&lt;a href="#bib1 bib2 bib3 bib4 bib5 bib6 bib7 bib8" target="_blank"&gt;1–8&lt;/a&gt;) and sample distribution in central (&lt;i&gt;n&lt;/i&gt; = 5) peripheral tumor tissues on 10-parameters flow cytometry analyses. &lt;b&gt;B,&lt;/b&gt; Distribution of each parameter as a single cell level. The color shows the median fluorescence...

&lt;div&gt;Abstract&lt;p&gt;In pancreatic ductal adenocarcinoma, the infiltration of CD8&lt;sup&gt;+&lt;/sup&gt; T cells within tumor microenvironment correlates with a favorable prognosis. However, significant proportion tumor-infiltrating become trapped desmoplastic stroma and lack reactivity. Here, we explored different T-cell subsets in tumors adjacent tissues. We identified subset cells, double positive (DP) for CD39 CD103 tumors, which has recently been described to display reactivity...

&lt;p&gt;Overall survival curves for T cells, MAIT cells and B cells.&lt;/p&gt;

&lt;p&gt;Overall survival curves for DP CD8 T cells and SPCD39, CD4 cells.&lt;/p&gt;