All the reports to date on anti-inflammatory activity of chitooligosaccharides (COS) are mostly based in vitro methods. In this work, two COS mixtures is characterized vivo (using balb/c mice), following carrageenan-induced paw edema method. This a widely accepted animal model acute inflammation evaluate effect drugs. Our data suggest that possess anti-inflammatoryactivity, which dependent dose and, at higher doses, also molecular weight. A single 500 mg/kg b.w. weight may be suitable treat...

Abstract Background Pterodon pubescens Benth seeds are commercially available in the Brazilian medicinal plant street market. The crude alcoholic extracts of this used folk medicine as anti-inflammatory, analgesic, and anti-rheumatic preparations. aim study was to evaluate contribution geranylgeraniol (C1) 6α, 7β-dihydroxyvouacapan-17β-oate methyl ester (C2) isolated from Benth. antinociceptive activity extract. Results Compounds C1 C2 demonstrated against writhing with intraperitoneal...

Harmicine is a β-carboline alkaloid isolated and identified as major active compound present in many plant species marine invertebrates. This exhibits wide spectrum of pharmacological activities, including antispasmodic, antipyretic, anticancer properties. report described the antinociceptive properties harmicine by means chemical experimental models order to evaluate use for pain relief. The results demonstrating potential analgesic administered intraperitoneally were shown with writhing...

Activity guided fractionation of Pterodon pubescens Benth. methylene chloride-soluble fraction afforded novel 6α-acetoxi 7β-hydroxy-vouacapan 1 and four known diterpene furans 2, 3, 4, 5. The compounds were evaluated for in vitro cytotoxic activities against human normal cells tumour cell lines UACC-62 (melanoma), MCF-7 (breast), NCI-H460 (lung, non-small cells), OVCAR-03 (ovarian), PC-3 (prostate), HT-29 (colon), 786-0 (renal), K562 (leukemia) NCI-ADR/RES (ovarian expressing phenotype...

A series of β-carboline derivatives bearing a substituted-carbohydrazide moiety at C-3 were synthesized and evaluated for their antitumor activity against eight human cancer cell lines. The N-(substituted-benzylidene)carbohydrazides showed, in general, greater than N-(alkylidene)carbohydrazide analogues. N(9)-methylation N-(substituted-benzylidene) carbohydrazides resulted decrease activity. Among compounds tested, the benzylidene-carbohydrazides 3, 4, 11, 13, 16, 21 22 most active,...

This study evaluated the pharmacological effect of association crude extract from fruits Pterodon pubescens (Pp) with essential oil Cordia verbenacea (Cv) in antinociception and anti-inflammatory experimental models. The effective doses each combinations used associations extracts were defined by acetic acid-induced writhing test. separate also on formalin Interaction between was assessed isobologram method. effects different concentrations (A50, A100 A200) test, tail flick hot plate....

The crude alcoholic extracts obtained from Pterodon pubescens Benth. seeds are widely used in Brazilian folk medicine as anti-inflammatory, analgesic, anti-rheumatic tonics and depurative preparations. We previously demonstrated the antinociceptive activity on writhing capsaicin, glutamate, hot-plate tests of two compounds isolated P. pubescens: geranylgeraniol (C1) 6α,7β-dihydroxyvouacapan-17β-oate methyl ester (C2). This work is a continuation previous study investigating possible...

Persistent inflammatory conditions can have severe pathological consequences. Although the use of nonsteroidal anti-inflammatory drugs (NSAIDs) is effective, it has side effects, particularly at gastrointestinal level. There then a high interest to identify natural compounds with no effects. The and anti-nociceptive activities hen egg lysozyme (LZ), both in its native form modified by heat treatment, chemically or enzymatic digestion been tested this study. carrageenan-induced model mice...

Artemisia annua L. has been used for many centuries in Chinese traditional medicine. Artemisinin, the active principle was first isolated and identified 1970s becoming global back bone to fight against malaria. Our research group previously developed an economic ecological friendly process obtain this compound. In pursuit also exploit residue generated throughout we further evaluated pharmacological potential of that extract. The alcoholic crude extract after artemisinin precipitation...

Summary The bioactivity and phytochemical composition of a partially purified extract açai (PPEA), concentrated in phenolic compounds (PC) without the presence macronutrients, were investigated. major PC quantified by UHPLC‐DAD‐LTQ‐Orbitrap MS‐MS/MS PPEA are anthocyanins. In vitro , showed cytostatic effect on K‐562 lymphoid leukaemia at concentration 40 μg mL −1 with GI 50 equal to 1.08 . vivo did not promote acute toxicity mice any doses tested. displayed gastroprotective activity rats...

ABSTRACT Introduction The oil obtained from Pterodon pubescens (Leguminosae) seeds are known to display anti‐cancer, anti‐dermatogenic and anti‐nociceptive activitiy. Phytochemical studies have demonstrated that its main constituents diterpenoids with voucapan skeletons. Considering the potential biological activities of oil, rapid efficient methods for assessing quality would facilitate certification control. Objective To develop a direct mass spectrometric fingerprinting method P. seed...

Pterodon pubescens Benth. (Pp) seeds, commercially available in Brazil, are used folk medicine anti-inflammatory, analgesic, and anti-rheumatic preparations. The present study demonstrated the antinociceptive properties of isomers 6α-hydroxy-7β-acetoxy-vouacapan-17β-oate methyl ester 6α-acetoxy-7β-hydroxy-vouacapan-17β-oate (C1), isolated from (Pp), employing different experimental models. A stability was performed to investigate relationship microencapsulation by spray-drying on maintenance...

Natural products produced by microorganisms have been an important source of new substances and lead compounds for the pharmaceutical industry. Chromobacterium violaceum is a Gram-negative β-proteobacterium, abundant in water soil tropical subtropical regions it produces violacein, pigment that has shown great potential. Crude extracts five Brazilian isolates sp (0.25, 2.5, 25, 250 µg/mL) were evaluated vitro antitumor activity assay with nine human tumor cells. Secondary metabolic profiles...

Pterodon pubescens Benth., popularly known as "sucupira", is traditionally used pain healing agent for many inflammatory diseases.The present study evaluated the in vivo antinociceptive and anti-inflammatory properties of sucupira's fruit dicloromethane extract (Pp) compared to aqueous (Ppa) folk medicine.Extracts' chemical characterization was performed by gas chromatography coupled mass spectra (GC-MS) detection.The standardized extracts were using experimental models with mice.The results...

The aim of this study was to evaluate the antiulcerative and antinociceptive activities milk proteins using induced gastric ulcer with ethanol rat model acetic acid-induced writhing mouse model. Casein (CN), (100, 300, 1000 mg kg-1) doses presented activity on a dose-dependent manner values 30.8%, 41.4%, 57.0% inhibition measured ulcerative lesions index (ULI), respectively. Whey protein concentrate (WPC), 48.9%, 65.5%, 68.22% ULI inhibition, CN, casein hydrolysates (CNH), WPC, whey (WPH),...

Os estudos e a crescente utilização dos fitoterápicos fizeram despertar em todo o mundo uma preocupação com sua eficácia, pois processamento inadequado das plantas pode ocasionar degradação moléculas conseqüente perda da atividade farmacológica. Entre as alternativas que podem ser utilizadas para preservar características de extratos vegetais, destaca-se encapsulação micropartículas. No presente levantamento, demonstraram-se brevemente os principais métodos microencapsulação, materiais...