Isabel Duarte Coutinho

ORCID: 0000-0002-7795-6129
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About
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Research Areas
  • Phytochemistry Medicinal Plant Applications
  • Soybean genetics and cultivation
  • Biofuel production and bioconversion
  • Plant Stress Responses and Tolerance
  • Plant responses to water stress
  • Plant nutrient uptake and metabolism
  • Sugarcane Cultivation and Processing
  • Information Science and Libraries
  • GABA and Rice Research
  • Lipid metabolism and biosynthesis
  • Essential Oils and Antimicrobial Activity
  • vaccines and immunoinformatics approaches
  • Plant Molecular Biology Research
  • Bee Products Chemical Analysis
  • Psidium guajava Extracts and Applications
  • Fern and Epiphyte Biology
  • Microbial Natural Products and Biosynthesis
  • Silicon Effects in Agriculture
  • Plant-Derived Bioactive Compounds
  • Adipose Tissue and Metabolism
  • Arsenic contamination and mitigation
  • Allelopathy and phytotoxic interactions
  • Meat and Animal Product Quality
  • Papaya Research and Applications
  • Phytochemistry and biological activity of medicinal plants

Universidade Estadual Paulista (Unesp)
2014-2024

Brazilian Agricultural Research Corporation
2016-2024

University of Aveiro
2023

Goethe University Frankfurt
2018

Universidade Estadual de Mato Grosso do Sul
2010

Universidade Federal de Mato Grosso do Sul
2008-2009

Sugarcane is an important agricultural crop in the economy of tropical regions, and Brazil has largest cultivated acreage world. accumulates high levels sucrose its stalks. Other compounds produced by sugarcane are currently not economic importance. To explore potential coproducts, we have studied chemical diversity genotypes, via metabolite profiling leaves NMR LC-DAD-MS. Metabolites were identified in-house public databases. From analysis 60 HPLC-fractionated extracts, LC-DAD-MS detected...

10.1021/acs.jafc.6b01210 article EN Journal of Agricultural and Food Chemistry 2016-05-06

The application of computer-aided drug discovery (CADD) approaches has enabled the new antimicrobial therapeutic agents in past. high prevalence methicillin-resistantStaphylococcus aureus(MRSA) strains promoted this pathogen to a high-priority for development. In sense, modern CADD techniques can be valuable tools search agents. We employed combination series machine learning (ML) select and evaluate potential compounds with antibacterial activity against methicillin-susceptible S. aureus...

10.1021/acs.jcim.4c00087 article EN Journal of Chemical Information and Modeling 2024-03-04

The anti-Mycobacterium tuberculosis activity of Campomanesia adamantium fruits extracts were evaluated. Six compounds, identified as flavanones and chalcones quantified by HPLC-DAD-UV. Promising antitubercular was observed with ethyl acetate extract (MIC 62.5 µg/mL) their fractions values ranging from 39 to above 250 µg/mL). better MIC result µg/mL associated two that contain bigger amounts 5,7-dihydroxy-6, 8-di-C-methylflavanone 2',4'-dihydroxy-3',5'-dimethyl-6'-methoxychalcone. These...

10.1590/s0100-40422009000500026 article EN cc-by-nc Química Nova 2009-01-01

Exopolysaccharide (EPS) are produced by a diverse of rhizobia species and has been demonstrated to be bioemulsifier with potential applications in the degradation hydrocarbons. In present study, attempts were made obtain new exopolysaccharide production Rhizobium tropici (SEMIA 4080 MUTZC3) strains during growth on hydrocarbon substrate. Under different cultivation conditions, high molecular weight exopolysaccharides from cultivated for 96h mainly consisted carbohydrates (79-85%) low...

10.1016/j.ijbiomac.2016.11.123 article EN publisher-specific-oa International Journal of Biological Macromolecules 2016-12-20

The essential oils from Campomanesia adamantium (Cambess.) O. Berg leaves, collected in the reproductive (flowering and fruit-bearing) vegetative stages, were characterized by GC-MS (Gas Chromatography-Mass Spectrometry). A total of 95 compounds identified. In stage (flowering) major constituents monoterpenes (limonene, α-pinene β-pinene) while during sesquiterpenes (bicyclogermacrene globulol). oil shows high antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa...

10.1590/s1984-82502009000400022 article EN cc-by Brazilian Journal of Pharmaceutical Sciences 2009-12-01

Campomanesia adamantium (Cambess.) O. Berg, Myrtaceae, é uma espécie nativa. As folhas são utilizadas na medicina popular. Os extratos hexânico e acetato de etila das mostraram pouca variação sua composição química em diferentes estações do ano associado com o desenvolvimento vegetal, enquanto extrato etanólico mostrou alteração significativa relação à química. Todos os etanólicos alta atividade antioxidante frente ao método DPPH moderada a para β-caroteno/ácido linoléico.

10.1590/s0102-695x2010000300006 article PT cc-by-nc-nd Revista Brasileira de Farmacognosia 2010-07-01

Metabolite profiling techniques play an important role in the phenotyping and diagnostic analysis of plants.

10.1039/c4ay01750a article EN Analytical Methods 2014-01-01

NMR and chromatography methods combined with mass spectrometry are the most important analytical techniques employed for plant metabolomics screening. Metabolomic analysis integrated to transcriptome screening add an extra dimension information flow from DNA RNA protein. The useful experiment in is proton spectra due high receptivity of 1H structural information, through proton–proton scalar coupling. Routinely, databases have been used identification primary metabolites, however, there...

10.1016/j.dib.2018.09.122 article EN cc-by Data in Brief 2018-10-15

Drought stress impacts soybean yields and physiological processes. However, the insertion of activated form AtAREB1 gene in cultivar BR16, which is sensitive to water deficit, improved drought response genetically modified plants. Thus, this study, we used 1H NMR solution solid-state investigate overexpressing under deficiency conditions. We achieved that drought-tolerant high content amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine,...

10.1021/acsomega.4c01796 article EN cc-by-nc-nd ACS Omega 2024-07-16

Introduction Solid-state NMR (SSNMR) spectroscopy methods provide chemical environment and ultrastructural details that are not easily accessible by other non-destructive, high-resolution spectral techniques. High-resolution magic angle spinning (HR-MAS) has been widely used to obtain the metabolic profile of a heterogeneous sample, combining resolution enhancement provided MAS in SSNMR with shimming locking procedures liquid-state NMR. Objective In this work, we explored feasibility using...

10.1002/pca.2702 article EN Phytochemical Analysis 2017-07-19

Methanolic, diethyl ether and n-hexane extracts of leaves four species Senna (S. gardneri, S. macranthera, splendida trachypus) were analyzed by gas chromatography coupled to mass spectrometry (GC-MS).Using linear retention indices spectral data, 34 compounds identified, including fatty acids, flavonoids, terpenoids steroids that not reported previously for these species.Additionally, the cytotoxicity against different tumor cell lines was determined.The then correlated with chemical...

10.5935/0103-5053.20160073 article EN cc-by Journal of the Brazilian Chemical Society 2016-01-01

Abstract The essential oils obtained from the leaves and flowers Campomanesia adamantium (Myrtaceae) collected in Mato Grosso do Sul flowering stage were analyzed by GC GC/MS. Eighty-two components identified leaf oil thirty-seven flower oil. constituent major was limonene (21.9%) ledol (20.9%)

10.1080/10412905.2008.9700041 article EN Journal of Essential Oil Research 2008-09-01

The BW has been largely used as a selection criterion in genetic programmes; however, increases can affect animal metabolism and metabolites. knowledge of how potential for growth affects the metabolites give footprint metabolism. This research aimed to evaluate effect post-weaning (GG) on performance, carcass traits serum metabolome non-castrated Nellore males during finishing phase. Forty-eight males, with divergent growth, were selected divided into two groups: high (HG; n = 24) low (LG;...

10.1017/s1751731119002520 article EN cc-by-nc-nd animal 2019-10-25

Objective: To guide health education about syphilis through TikTok digital media, addressing the forms of contamination, signs and symptoms, diagnosis, prevention treatment. Methods: The method used for development this project was Arco de Maguerez, which consists five stages that are observation reality, key points, theorizing, solution hypothesis application reality. Experience report: Social media currently excellent tools dissemination information, because it is possible to create,...

10.69849/revistaft/ni10202411161625 article EN Revista fisio&terapia. 2024-11-16

Com base no conceito de economia circular, resíduos fontes vegetais, entre eles manga, são reaproveitados e podem ser extraídas algumas biomoléculas como pectina celulose para serem utilizadas em aplicações biotecnológicas, também a indústria cosmética, até mesmo biomateriais. Neste contexto, foi realizada caracterização térmica, morfológica reológica biopolímeros puros misturas gel analisar seu potencial produzir membranas, scaffolds ou estruturas bioimpressas. A análise FTIR demonstrando...

10.52466/ijamb.v6i2.119 article PT International Journal of Advances in Medical Biotechnology - IJAMB 2024-12-01

Cariniana estrellensis (Raddi) Kuntze seedlings have been used in forest restoration programs the Atlantic Forest biome. However, despite economic and ecological relevance of this species, to date no general method for rapid identification primary secondary metabolites C. leaves has reported. In work, we explore feasibility using analysis by nuclear magnetic resonance techniques liquid chromatography coupled diode array, charged aerosol, mass detection. The main identified were hydrolysable...

10.21577/0103-5053.20220083 article EN cc-by Journal of the Brazilian Chemical Society 2022-01-01

Abstract Drought stress impacts soybean yield and physiological processes. However, the insertion of activated form AtAREB1 gene in cultivar BR16, which is sensitive to water deficit improved drought response genetically-modified plants under glasshouse conditions. Thus, this study used 1 H NMR solution solid-state investigate genetically modified overexpressing AREB deficiency We achieved that drought-tolerant yields high content amino acids isoleucine, leucine, threonine, valine, proline,...

10.21203/rs.3.rs-3335098/v1 preprint EN cc-by Research Square (Research Square) 2023-09-13
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