A5033524951- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Thermoregulation and physiological responses
- Sodium Intake and Health
- Cardiovascular Health and Disease Prevention
- Blood Pressure and Hypertension Studies
- Apelin-related biomedical research
- Estrogen and related hormone effects
- Nitric Oxide and Endothelin Effects
- Stress Responses and Cortisol
- Chronic Kidney Disease and Diabetes
- Vitamin D Research Studies
- Medical and Biological Ozone Research
- Human Health and Disease
- Heart Rate Variability and Autonomic Control
- Eicosanoids and Hypertension Pharmacology
- Birth, Development, and Health
- Infrared Thermography in Medicine
- Dialysis and Renal Disease Management
- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- Alkaline Phosphatase Research Studies
- Cystic Fibrosis Research Advances
- Breast Cancer Treatment Studies
- Laser Applications in Dentistry and Medicine
Tufts Medical Center
2015-2024
Beth Israel Deaconess Medical Center
2018
Harvard University
2018
University of Florida
2018
University of Delaware
2010-2014
Harry S. Truman Memorial Veterans' Hospital
2013
University of Missouri
2013
DuPont (United States)
2013
Plymouth State University
2010
Université Claude Bernard Lyon 1
1977-1978
Preeclampsia is a hypertensive disorder of pregnancy in which patients develop profound sensitivity to vasopressors, such as angiotensin II, and associated with substantial morbidity for the mother fetus. Enhanced vasoconstrictor elevations soluble fms-like tyrosine kinase 1 (sFLT1), circulating antiangiogenic protein, precede clinical signs symptoms preeclampsia. Here, we report that overexpression sFlt1 pregnant mice induced II hypertension by impairing endothelial nitric oxide synthase...
Excess dietary sodium has been linked to the development of hypertension and other cardiovascular diseases. In humans, effects consumption on endothelial function have not separated from blood pressure. The present study was designed determine if intake affected endothelium-dependent dilation (EDD) independently changes in pressure.Fourteen healthy salt-resistant adults were studied (9M, 5F; age 33 ± 2.4 years) a controlled feeding study. After baseline run-in diet, participants randomized...
Key points Pre‐clinical studies suggest that acute dietary sodium loading impairs vascular function without alterations in blood pressure; however, human data are lacking. In this study, normotensive salt‐resistant adults participated a controlled feeding which they consumed low‐sodium diet for 1 week and high‐sodium week, random order. During each diet, microvascular was assessed. Here we report the novel finding of sodium‐induced impairments independent pressure healthy adults. We...
Hypertension is nearly universal yet poorly controlled in the elderly despite proven benefits of intensive treatment. Mice lacking mineralocorticoid receptors smooth muscle cells (SMC-MR-KO) are protected from rising blood pressure (BP) with aging, normal renal function. Vasoconstriction attenuated aged SMC-MR-KO mice, thus they were used to explore vascular mechanisms that may contribute hypertension aging. MicroRNA (miR) profiling identified miR-155 as most down-regulated miR aging...
The incidence of obesity is rising, particularly among women. Microvascular dysfunction more common with female sex, obesity, and hyperlipidemia predicts adverse cardiovascular outcomes, but the molecular mechanisms are unclear. Because associated mineralocorticoid receptor (MR) activation, we tested hypothesis that MR in endothelial cells contribute to sex differences resistance vessel response cardiometabolic risk factors.Male cell-specific knockout mice MR-intact littermates were...
Stiffening of the vasculature with aging is a strong predictor adverse cardiovascular events, independent all other risk factors including blood pressure, yet no therapies target this process. MRs (mineralocorticoid receptors) in smooth muscle cells (SMCs) have been implicated regulation vascular fibrosis but not explored aging. Comparing SMC-MR-deleted male mice to MR-intact littermates at 3, 12, and 18 months age, we demonstrated that aging-associated stiffening are mitigated by MR...
Aging is associated with heart and vascular dysfunction that contributes to cardiovascular disease (CVD) risk. Clinical data support a sexual dimorphism in the time course of aging-associated CVD. However, mechanisms driving sex differences aging whether they can be modeled mice have not been explored. Mineralocorticoid receptors (MRs) regulate blood pressure, we previously demonstrated male MR expression increases mouse vessels smooth muscle cell-specific deletion (SMC-MR-KO) protects from...
We sought to determine whether oxidative stress or a relative deficit of l-arginine plays role in reducing cutaneous vasodilation response local heating chronic kidney disease (CKD). Eight patients with stage 3-4 CKD and eight age- sex-matched healthy control (HC) subjects were instrumented four microdialysis (MD) fibers for the delivery 1) Ringers solution (R), 2) 20 mM ascorbic acid (AA), 3) 10 (l-Arg), 4) N(G)-nitro-l-arginine methyl ester (l-NAME). Red blood cell (RBC) flux was measured...
To determine the general appearance of normal axillary lymph nodes (LNs) in real-time tissue sonoelastography and to explore method′s potential value prediction LN metastases. Axillary LNs healthy probands (n=165) metastatic breast cancer patients (n=15) were examined with palpation, B-mode ultrasound, Doppler (assessment elasticity cortex medulla). The distributions compared sensitivity (SE) specificity (SP) calculated. In an exploratory analysis, positive negative predictive values (PPV,...
Oxidative stress promotes vascular dysfunction in chronic kidney disease (CKD). We utilized the cutaneous circulation to test hypothesis that reactive oxygen species derived from NADPH oxidase and xanthine impair nitric oxide (NO)-dependent vasodilation CKD. Twenty subjects, 10 stage 3 4 patients with CKD (61 ± yr; 5 men/5 women; eGFR: 39 ml·min(-1)·1.73 m(-2)) healthy controls (55 2 men/6 >60 were instrumented intradermal microdialysis fibers for delivery of 1) Ringer solution (Control), 2)...
Abstract Aims Vascular stiffness increases with age and independently predicts cardiovascular disease risk. Epigenetic changes, including histone modifications, accumulate but the global pattern has not been elucidated nor are regulators known. Smooth muscle cell-mineralocorticoid receptor (SMC-MR) contributes to vascular in ageing mice. Thus, we investigated regulatory role of SMC-MR epigenetics stiffness. Methods results Mass spectrometry-based proteomic profiling all modifications...
A reduction in progenitor cell populations that help preserve vascular continuity and induce vascularization may accentuate endothelial apoptosis dysfunction, ultimately contributing to organ failure increased cardiovascular disease chronic kidney (CKD). We hypothesized CD45+ myeloid CD34+ hematopoietic circulating (CPC) subpopulations would be reduced, peripheral blood mononuclear (PBMNC) colony-forming units (CFU) impaired, flow-mediated dilation (FMD) impaired patients with...
OBJECTIVE: Elevated levels of the hormone aldosterone are associated with increased risk myocardial infarction and stroke in humans progression inflammation atherosclerotic plaques animal models. Aldosterone acts through mineralocorticoid receptor (MR) which is expressed vascular smooth muscle cells (SMCs) where it promotes SMC calcification chemokine secretion vitro. The objective this study to explore role MR specifically SMCs atherosclerosis vivo apolipoprotein E knockout (ApoE-/-) mouse...
We tested the hypothesis that microdialysis of hypertonic saline would attenuate skin blood flow response to local heating. Seventeen healthy subjects (23 ± 1 years old) were studied. In one group (n= 9), four fibres placed in forearm and infused with following: (1) Ringer solution; (2) normal (0.9% NaCl); (3) (3% (4) 10 mm l-NAME. A second 8) was saline; +l-NAME; +l-NAME. Red cell flux measured via laser Doppler flowmetry during heating 42°C. Site-specific maximal vasodilatation determined...
In patients hospitalized with acute heart failure, temporary serelaxin infusion reduced 6-month mortality through unknown mechanisms. This study therefore explored the cardiovascular effects of administration in mice subjected to angiotensin II (AngII)/L-NG-nitroarginine methyl ester (L-NAME) failure model, both during and 19 days post-serelaxin infusion. Serelaxin did not alter AngII/L-NAME-induced cardiac hypertrophy, geometry, or dysfunction. However, serelaxin-treated had perivascular...
1. The influence of thryoid function on the development hypertension was studied in strains spontaneously hypertensive (SH) and normotensive rats. 2. Surgical thyroidectomy decreased systolic blood pressure more markedly SH rats than effects oral administration 5 100 micrograms thyroxine 24 h-1 g-1 were thyroidectomized animals. In two returned to control levels only after larger dose. 3. evolution body weight, total plasma tri-iodothyronine (T3) tetraiodothyronine (T4) concentrations...
Introduction: Angiotensin II (AngII) affects cardiovascular health, mediating impacts through AngII type 1 (AT1R) and 2 (AT2R) receptors. The present study investigated sex aging-related differences in microvascular receptor function mice humans. Methods: Mesenteric resistance arteries (MRA) were isolated from 3-, 12-, 18-month-old female male C57/Bl6 mice. Wire myography was used to measure vasoconstriction vasodilation an AT2R agonist (compound 21, C21). Seven healthy adults (3...
Background: Arterial stiffness is an independent predictor of cardiovascular events and mortality. Clinical data shows arterial increases rapidly after menopause, but mechanisms that mediate this change are not well known. The effect ovariectomy (ovx) in rodent models inconsistent the literature. We hypothesize loss estradiol (E2) contributes to ovx-induced stiffening a mouse model via fibrotic mechanisms, restoration E2 will prevent these changes. Methods: Eight-week-old female C57/Bl6J...