Hypertension is nearly universal yet poorly controlled in the elderly despite proven benefits of intensive treatment. Mice lacking mineralocorticoid receptors smooth muscle cells (SMC-MR-KO) are protected from rising blood pressure (BP) with aging, normal renal function. Vasoconstriction attenuated aged SMC-MR-KO mice, thus they were used to explore vascular mechanisms that may contribute hypertension aging. MicroRNA (miR) profiling identified miR-155 as most down-regulated miR aging...

Arteriolar vasoreactivity tightly regulates tissue-specific blood flow and contributes to systemic pressure (BP) but becomes dysfunctional in the setting of cardiovascular disease. The mineralocorticoid receptor (MR) is known regulate BP via kidney by vasoconstriction smooth muscle cells. Although endothelial cells (EC) express MR, contribution EC-MR resistance vessel function remains unclear. To address this, we created a mouse with MR specifically deleted from EC (EC-MR knockout...

Many in vitro studies have investigated the mechanism by which mechanical signals are transduced into biological that regulate bone homeostasis via periodontal ligament fibroblasts during orthodontic treatment, but results not been systematically reviewed. This review aims to do this, considering parameters of various loading approaches and their effects on osteogenic osteoclastogenic properties fibroblasts.Specific keywords were used search electronic databases (EMBASE, PubMed, Web Science)...

Ack/Ack1 is a nonreceptor protein tyrosine kinase that comprises core, an SH3 domain, Cdc42-binding region, Ralt homology and proline-rich region. Here we describe detailed characterization of the Ack as well chromosomal localization human (chromosome 3q29) primary structure murine Ack. We demonstrate ubiquitously expressed, with highest expression seen in thymus, spleen, brain. Activation integrins by cell adhesion on fibronectin leads to strong phosphorylation activation Upon stimulation...

Gestational diabetes mellitus (GDM) is a metabolic and low-grade inflammatory disease most commonly found in pregnant women with high body mass index non-Caucasian ethnicities; however, not all of high-risk groups develop GDM. We hypothesized that regulatory T cells (Tregs) might present role suppressing GDM development. To this end, 55 at early pregnancy (first trimester) were recruited, 21 them developed while the other 34 did not. Compared to those subjects who (non-GDM), patients...

Abstract Background A new two-piece abutment design consisting of an upper prosthetic component and tissue-level base has been introduced; however, the biomechanical behavior such a not documented. This study aimed to investigate effect on stress in implant components surrounding bone, as well its influence microgap formation. Methods To simulate models mandibular left first molar area, we established nine experimental groups that included three bone qualities (type II, III, IV)...

The aim of this study was to investigate the role LAG1 longevity-assurance homologue 2 (LASS2) in papillary thyroid cancer (PTC).Immunohistochemistry staining conducted explore expression levels LASS2 PTC tissues and adjacent normal nodular goiter tissues. Western blotting RT-qPCR were performed three cell lines (TPC-1, K1, BCPAP). An Adv-LASS2-GFP recombinant adenovirus vector constructed transduced into BCPAP cells. Then CCK-8 assay, colony formation cycle distribution, apoptosis...

D2-40 has been shown a selective marker for lymphatic endothelium, but also in the benign cervical basal cells. However, application of immunoreactivity cells identifying grade intraepithelial neoplasia (CIN) not evaluated. In this study, immunoreactive patterns D2-40, compared with p16INK4A, which is currently considered as useful cancers and their precancerous diseases, were examined total 125 specimens including 32 CIN1, 37 CIN2, 35 CIN3, 21 normal tissue. p16INK4A immunoreactivities...

<b>Background/Aims:</b> Deoxyschizandrin as one of the most important component Schisandra chinensis (Turcz.) Baill plays an immunomodulatory role in a variety diseases, yet its ulcerative colitis remains to be elucidated. We aimed investigate deoxyschizandrin DSS-induced mice. <b>Patients and Methods</b>: In present study, inflammation model cells was constructed confirm anti-inflammatory effect deoxyschizandrin. Then mouse with Dextran sulfate sodium (DSS)-induced constructed, effects on...

Purpose: To evaluate the effects of 1,25 dihydroxy vitamin D3 (1,25(OH)2D3) on content triglyceride (TG), as well gene and protein expressions adiponectin receptor 2 (AdipoR2), p38 mitogen-activated kinase (P38MAPK), lipoprotein lipase (LPL) in liver rats with type diabetes mellitus (T2DM) so to provide theoretical basis for exploring mechanism by which 1,25(OH)2D3 regulates TG. Methods: Wistar were divided into four groups (n=25), different treatments detected AdipoR2, p38MAPK, LPL tissue...

Abstract Oligosaccharidoses are a group of lysosomal storage disorders characterized by abnormal and excretion incompletely processed glycan structures. As with other inherited metabolic disorders, early diagnosis initiation treatment essential for optimizing outcomes. Biochemical investigation suspected oligosaccharidoses has traditionally included thin layer chromatography to detect the presence disease‐specific free oligosaccharides in urine; however, this qualitative method long been...

Puerarin has potential therapeutic effects on diabetic nephropathy (DN), but the effectiveness as a treatment for DN and underlying mechanism remain to be elucidated. The DN-like model induced by high glucose in vitro streptozotocin vivo were used observe effect of puerarin. results showed that puerarin can enhance activity HBZY-1 cells reduce apoptosis. enzymelinked immunosorbent assay biochemical improve symptoms. Using hematoxylin eosin staining stain kidney tissues confirmed protective...

BACKGROUND Excessive alcohol consumption can cause hepatocellular injury. ATPase II (ATP8A1) display an ATP-dependent phospholipid translocase activity. However, the function of ATP8A1 in hepatocyte injury is still unclear. In present study we explored effect on ethanol-induced MATERIAL AND METHODS A human strain, HL-7702, was pretreated by ethanol with gradient concentration for 2, 4, 8, and 12 h, were then divided into 6 groups after cells transfected. We detected cell viability use Cell...

Objective 　To research the relationship between human papillomavirus (HPV) infection and oncogenesis of primary squamous carcinoma lung. Methods 　50 formalin-fixed paraffin-embedded tissues cell lung , 30 samples areas epithelium metaplasia near tumors normal bronchial mucosa were detected for presence HPV DNA by polymerase chain reaction (PCR) . Results 　In these positive detection rate was 26 % 36. 7 10 respectively. Of HPV16 highest ratio. The HPV6/ 11 in had no significant difference...

Objective Activity of vitamin D is correlated with type 2 diabetes mellitus and hyperlipidemia,but the concrete relationship 1,25-dihydroxyvitamin D3 [1,25 (OH) D3] triglyceride unknown.This study was to observe gene protein expression peroxisome proliferator-activated receptor α(PPAR-α),hepatocyte neclear factor-4α (HNF-4α),sterol regulatory element binding proteins-1C (SREBP-1 C),apolipoprotein (apoA5) during 1,25 (OH)2D3 regulation in liver rat,as well as investigate mechanism 2D3...

Objective To observe the gene and protein expression of PPARα,sterol regulatory element binding proteins-1C (SREBP-1C),and apolipoprotein (apo) A5 under adiponectin intervention,as well as triglyceride level in liver diabetic rats.Methods Sixty male Wistar rats were assigned into 3 groups random:diabetic group(DM,n =20),adiponectin treated group (Ad-APN + DM,n =20),and control (CON,n =20).10 from each taken to detect PPARα,SREBP-1C,and apoA5 by real time-fluorescent quantitation reverse...

Objective To study the value of using endoscopy iodine staining combined detecting expression p53, survivin and telomerase in diagnosis early esophageal carcinoma precancerous lesions. Methods Three hundred patients who received routine gastrescopy had high risk were examined by dyeing on their mucosa with 2. 5% Lugol solu-tion. Multiple biopsies taken from unstained or light-stained areas when diameters over 5mm for pathology diagnosis. The detected immunohistochemical staining. Results...