COVID‐19, a global pandemic, has caused over 750,000 deaths worldwide as of August 2020. A vaccine or remedy for SARS‐CoV‐2, the virus responsible is necessary to slow down spread and lethality COVID‐19. However, there currently no effective treatment available against SARS‐CoV‐2. In this report, we demonstrated that EGCG theaflavin, main active ingredients green tea black tea, respectively, are potentially inhibit SARS‐CoV‐2 activity. Coronaviruses require 3CL‐protease cleavage its...

The COVID-19 pandemic has resulted in a huge number of deaths from 2020 to 2021; however, effective antiviral drugs against SARS-CoV-2 are currently under development. Recent studies have demonstrated that green tea polyphenols, particularly EGCG, inhibit coronavirus enzymes as well replication vitro. Herein, we examined the inhibitory effect polyphenols on mouse model. We used epigallocatechin gallate (EGCG) and containing more than 60% catechin (GTP60) human OC43 (HCoV-OC43) surrogate for...

Epigallocatechin gallate (EGCG) is a major catechin found in green tea, and there mounting evidence that EGCG potentially useful for the treatment of coronavirus diseases, including disease 2019 (COVID-19). Coronaviruses encode polyproteins are cleaved by 3CL protease (the main protease) maturation. Therefore, regarded as target antivirals against coronaviruses. constituent brewed several studies have reported inhibits enzymatic activity protease. Moreover, has been to regulate other...

Prostaglandin regulation is known to play a pivotal role in tumorigenesis; however, the contributions of prostaglandin-metabolizing enzyme 15-hydroxyprostaglandin dehydrogenase (HPGD) cancer development remain poorly understood. In this study, we investigate effects HPGD on cell viability, proliferation, anchorage-independent growth, and migration triple-negative breast (TNBC), an aggressive subtype cancer. Overexpression human TNBC cells resulted both positive negative proliferation colony...

AMP-activated protein kinase (AMPK) is an important regulator of cellular energy homeostasis, and AMPK contributes to cell growth, apoptosis, autophagy. Although most studies have been performed using two-dimensional (2D) culture, recent demonstrated that the three-dimensional (3D) spheroid technique helpful in various research fields, such as tumor biology, due its resemblance 3D tissue structure. However, role formation has not characterized clearly. This study used knockout line examine...

The coronavirus disease (COVID-19) pandemic has resulted in more than six million deaths by October 2022. Vaccines and antivirals for severe acute respiratory syndrome 2 are now available; however, effective antiviral drugs required treatment. Here, we report that a potent AMP-activated protein kinase (AMPK) inhibitor, compound C/dorsomorphin, inhibits the replication of human OC43 strain (HCoV-OC43). We examined HCoV-OC43 control AMPK-knockout (KO) cells found virus decreased AMPK-KO cells....

6-Azauridine (6-AZA), a pyrimidine nucleoside analogue, is known to exhibit both antitumor and antiviral activities. Although 6-AZA was discovered more than 60 years ago, the cellular effects of this compound are yet be elucidated. Here, we report that regulates autophagy-mediated cell death in various human cancer cells, where treatment activates autophagic flux through activation lysosomal function. Furthermore, exhibited cytotoxicity all cells studied, although mechanisms action were...

C1q and TNF-related 1 (C1QTNF1/CTRP1) is an adiponectin-associated protein belonging to the C1q/TNF-related family. Recent studies have shown that (CTRP) family involved in cancer progression; however, specific role of CTRP1 tumor progression has not yet been elucidated. To examine progression, we generated knockout A549 HCT116 cell lines, which reduced expression levels nuclear factor (NF)-κB-dependent metastasis-promoting transcripts. We demonstrated inhibited proliferation invasion...

The coronavirus disease 2019 (COVID-19) pandemic has caused more than six million deaths worldwide since 2019. Although vaccines are available, novel variants of expected to appear continuously, and there is a need for effective remedy disease. In this report, we isolated eupatin from Inula japonica flowers showed that it inhibits the 3 chymotrypsin-like (3CL) protease as well viral replication. We treatment SARS-CoV-2 3CL-protease, computational modeling demonstrated interacts with key...

Coronavirus disease 2019 (COVID-19) is an infectious caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). 3CLpro a key enzyme in coronavirus proliferation and treatment target for COVID-19. In vitro silico, compounds 1-3 from Glycyrrhiza uralensis had inhibitory activity binding affinity 3CLpro. These decreased HCoV-OC43 cytotoxicity RD cells. Moreover, they inhibited viral growth reducing the amounts of necessary proteins (M, N, RDRP). Therefore, are inhibitors proliferation.

Feline Coronavirus (FCoV) is a viral pathogen of cats and highly contagious virus. Cats in cattery can be infected by up to 100%, even household are 20–60%. Some strains FCoV known induce fatal disease named Infectious Peritonitis (FIP). However, no effective treatments available. We demonstrated that compound C (dorsomorphin) potentially inhibit feline coronavirus replication. Compound treatment decreased the FCoV-induced plaque formation cytopathic effect FCoV-infected cells. also...

Highlights•Selaginella tamariscina extract (STE) inhibits SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) activity.•STE treatment coronavirus replication.•Amentoflavone, a central component of STE, RdRp activity.AbstractThe pandemic caused millions deaths due to its prominent infectivity and mortality. Although the vaccines medicines for are on market, new variants like influenza expected reemerge continuously. Therefore, effective inexpensive will be required respond variants. Here, we used...

ABSTRACT COVID-19 has caused over 900,000 deaths worldwide as of September 2020, and effective medicines are urgently needed. Lopinavir was identified an inhibitor the HIV protease, a lopinavir-ritonavir combination therapy reported to be beneficial for treatment SARS MERS. However, recent clinical tests could not prove that COVID-19. In this report, we examined effect lopinavir ritonavir activity purified main protease (Mpro) protein SARS- CoV-2, causative virus Unexpectedly, did inhibit...

Mounting evidence supports the relationship between obesity and cancer. However, molecular mechanisms linking with cancer remain largely uninvestigated. In this study, we demonstrate that expression of C1q/TNF-related protein 1 (CTRP1), an adiponectin paralogue, contributes to tumor growth by regulating suppressor p53. our obese mice on a high-fat diet showed higher serum CTRP1 levels. Through in vitro experiments, secreted form culture medium decreased p53 p53-dependent transcription cells....

The well‐known tumor suppressor p53 inhibits the formation of various cancers by inducing cell cycle arrest and apoptosis. Although mutations are commonly found in many cancers, is functionally inactivated cells that retain wild‐type p53. Here, we show ligand numb protein X1 (LNX1) inhibited p53‐dependent transcription decreasing half‐life We generated LNX1 knockout (KO) cancer (A549, HCT116, MCF7) using clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated 9...

5H-Benzimidazo[1,2-c]quinazoline-6-thione (BI–QT), was synthesized as a benzimidazole-based probe to detect H2S. BI–QT exhibits fluorescent "turn-on" response in DMSO/H2O (9:1, HEPES 10 mM, pH 7.4) upon the addition of The can determine micromolar (0–600 µM) H2S concentrations aqueous systems, with detection limit 1.12 µM. Interestingly, exhibited an ultrafast H2S, maximum intensity achieved almost instantly when exposed is largely unaffected by and responds reliably over wide 4–11 range,...

The ligand of numb-protein X1 (LNX1) acts as a proto-oncogene by inhibiting p53 stability; however, the regulation LNX1 expression has not been investigated. In this study, we screened chemicals to identify factors that potentially regulate expression. We found levels were decreased DNA damage, including cisplatin. Upon treatment with lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA), increased. addition, cell-cycle progression increased upon expression; S G2/M populations...