A5050506793- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Ischemic Stroke Management
- Thermal Regulation in Medicine
- Cerebrovascular and Carotid Artery Diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurological Disease Mechanisms and Treatments
- Barrier Structure and Function Studies
- Cardiac Ischemia and Reperfusion
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
- Advanced MRI Techniques and Applications
- Neonatal and fetal brain pathology
- Pharmacological Effects and Assays
- Pesticide Residue Analysis and Safety
- Intracranial Aneurysms: Treatment and Complications
- Anesthesia and Neurotoxicity Research
- Autophagy in Disease and Therapy
- Biochemical effects in animals
- Cardiac Arrest and Resuscitation
- Cancer-related molecular mechanisms research
- Invertebrate Immune Response Mechanisms
- Meat and Animal Product Quality
- Mesenchymal stem cell research
- Endoplasmic Reticulum Stress and Disease
- Neonatal Respiratory Health Research
Shandong First Medical University
2024-2025
Capital Medical University
2015-2024
Massachusetts General Hospital
2019-2024
Harvard University
2019-2024
Huazhong Agricultural University
2016-2021
Chinese Institute for Brain Research
2017-2021
Beijing Anzhen Hospital
2017
Wayne State University
2017
Medical University of South Carolina
2017
Xian Central Hospital
2017
Remote ischemic preconditioning (RIPC) can inhibit recurrent events effectively in patients with acute or chronic cerebral ischemia. However, it is still unclear whether RIPC impede injury after carotid artery stenting (CAS) severe stenosis.Subjects stenosis were recruited this randomized controlled study, and assigned to RIPC, sham, no intervention (control) groups. All subjects received standard medical therapy. Subjects the sham groups underwent twice daily, respectively, for 2 weeks...
Abstract After stroke, peripheral immune cells are activated and these systemic responses may amplify brain damage, but how the injured sends out signals to trigger inflammation remains unclear. Here we show that a brain-to-cervical lymph node (CLN) pathway is involved. In rats subjected focal cerebral ischemia, lymphatic endothelial proliferate macrophages rapidly in CLNs within 24 h, part via VEGF-C/VEGFR3 signalling. Microarray analyses of isolated endothelium from ischemic mice confirm...
The mitochondrial unfolded protein response (UPR
Beyond neuronal injury, cell death pathways may also contribute to vascular injury after stroke. We examined protein networks linked major and identified SLC22A17 (solute carrier family 22 member 17) as a novel mediator that regulates endothelial tight junctions ischemia inflammatory stress.
Nearly all stroke neuroprotection modalities, including selective intra-arterial cooling (SI-AC), have failed to be translated from bench bed side. Potentially overlooked reasons may biological gaps, inadequate attention reperfusion states and mismatched neurological benefits. To advance translation, we describe a novel thrombus-based model in adult rhesus macaques. Intra-arterial thrombolysis with tissue plasminogen activator leads three clinically relevant outcomes – complete, partial, no...
Abstract The concept of the neurovascular unit emphasizes importance cell-cell signaling between neural, glial, and vascular compartments. In neurogenesis, for example, brain endothelial cells play a key role by supplying trophic support to neural progenitors. Here, we describe surprising phenomenon where may release trans-differentiation signals that convert astrocytes into progenitor in male mice after stroke. After oxygen-glucose deprivation, microvesicles containing pro-neural factor...
Hypothermia has shown promise as a neuroprotective strategy for stroke. The use of whole body hypothermia limited clinical utility due to many severe side effects. Selective brain cooling, or local hypothermia, been previously proposed an alternative treatment strategy. This study investigated the safety, feasibility, and efficacy selective induced by infusion ice-cold lactated Ringer's solution in rhesus monkeys.Eight male monkeys were used this study. Brain temperature territory supplied...
To further investigate and improve upon current stroke models in nonhuman primates, infarct size, neurologic function survival were evaluated two endovascular ischemic sixteen rhesus monkeys. The first method utilized a micro-catheter or an inflatable balloon to occlude the M1 segment six In second model, autologous clot was injected via into ten MRI scanning performed on all monkeys both at baseline 3 hours after onset of ischemia. Spetzler functions assessed post-operatively, selective...
Background and Purpose- Induction of hypothermia as a stroke therapy has been limited by logistical challenges. This study was designed to determine the hypothermic neuroprotective efficacy infusing cold saline directly into internal jugular (IJ) vein compare effects IJ those achieved intracarotid artery in an ischemic model. Methods- The right middle cerebral occluded rats using intraluminal filament. Immediately following reperfusion, isotonic through microcatheter or over 30 minutes....
We aimed to evaluate the safety and effectiveness of short-term remote ischemic postconditioning (RIPC) in acute stroke monkey models.Acute monkeys were allocated four groups based on number limbs exposed RIPC. RIPC was initiated by 5-min cuff inflation/deflation cycles target limb(s) for 5-10 bouts. Vital signs, skin integrity, brain MRI, serum levels cardiac enzymes (myoglobin, creatine kinase [CK], CK-muscle/brain [CK-MB]), one inflammatory marker (high-sensitivity C-reactive protein...
Physical hypothermia has long been considered a promising neuroprotective treatment of ischemic stroke, but the treatment's various complications along with impractical duration and depth therapy significantly narrow its clinical scope. In present study, model reversible right middle cerebral artery occlusion (MCAO) for 2 h was used. We combined (33-35 °C 1 h) phenothiazine neuroleptics (chlorpromazine & promethazine) as additive neuroprotectants, aim augmenting efficacy while only using...
Hypothermia is considered as a promising neuroprotective treatment for ischemic stroke but with many limitations. To expand its clinical relevance, this study evaluated the combination of physical (ice pad) and pharmacological [transient receptor potential vanilloid channel 1 (TRPV1) agonist, dihydrocapsaicin (DHC)] approaches faster cooling stronger neuroprotection. A total 144 male Sprague Dawley rats were randomized to 7 groups: sham (n=16), only (n=24), hypothermia at 31ºC 3 h after...
Acrylamide (ACR) is a water-soluble chemical applied in industrial and laboratory processes. The neurotoxicity induced by acrylamide involves both peripheral central nervous system. Hence, there growing urgency to investigate the mechanisms of acrylamide-induced search novel therapeutic target for nerve repair. effects ACR on proliferation, reactive oxygen species (ROS) iron production dorsal root ganglia (DRG) neurons Schwann cells were determined. 5-Ethynyl-2'-deoxyuridine (EDU) staining...
Background: Animal and clinical studies have shown that remote ischemic conditioning (RIC) has protective effects for cerebral vascular diseases, with induced humoral factor changes in the peripheral blood. However, many findings are heterogeneous, perhaps due to differences RIC intervention schemes, enrolled populations, sample times. This study aimed examine RIC-induced plasma proteome using rhesus monkey models of strokes. Methods: Two adult monkeys autologous blood clot-induced middle...
Reperfusion therapy inevitably leads to brain–blood barrier (BBB) disruption and promotes damage despite its benefits for acute ischaemic stroke (AIS). An effective brain cytoprotective treatment is still needed as an adjunct reperfusion therapy. Here, we explore the potential of therapeutic hypothermia (HT) in attenuating early BBB leakage improving neurological outcomes. Mild HT was induced during peri-recanalization stages a mouse model transient middle cerebral artery occlusion...