A5000958906- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Immunodeficiency and Autoimmune Disorders
- Leprosy Research and Treatment
- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
- Parasites and Host Interactions
- Pleural and Pulmonary Diseases
- Quinazolinone synthesis and applications
- Child Nutrition and Water Access
- Infectious Diseases and Mycology
- HIV/AIDS drug development and treatment
- Antibiotic Resistance in Bacteria
- Amoebic Infections and Treatments
- Bacteriophages and microbial interactions
- Chronic Lymphocytic Leukemia Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pneumothorax, Barotrauma, Emphysema
- Food Safety and Hygiene
- Dermatological and Skeletal Disorders
- Ginseng Biological Effects and Applications
- Neutropenia and Cancer Infections
Fundação Oswaldo Cruz
2013-2024
Khyber Medical College
2024
National Centre for Research
2017
Public Health Laboratory
2012
HealthPartners
2012
University Gama Filho
2011
Universidade Federal Fluminense
2001
Berkeley Public Health Division
1999
University of California, Berkeley
1999
Universidade Federal do Rio de Janeiro
1999
Lately, much effort has been made to find mRNA biomarkers for tuberculosis (TB) disease/infection with microarray-based approaches. In a pilot investigation, through RNA sequencing technology, we observed prominent modulation of DOCK9, EPHA4, and NPC2 abundance in the blood TB patients. To corroborate these findings, independent validations were performed cohorts from different areas. Gene expression levels evaluated by quantitative real-time PCR (Brazil, n = 129) or reanalysis public...
Tuberculosis is the infectious disease with worldwide largest burden and there remains a great need for better diagnostic biomarkers to detect latent active M. tuberculosis infection. RNA molecules hold promise in this regard, as their levels of expression may differ considerably between infected uninfected subjects. We have measured changes four major classes small noncoding RNAs blood samples from patients different stages TB found that, addition miRNAs (which are known be highly regulated...
It has been widely accepted, that pyrazinamide (PZA) resistance in Mycobacterium tuberculosis is correlated with mutations the pncA gene. But since years researchers have puzzled by fact up to 30% of PZA resistant strains do not show any correlation between and gene, thus may vary geographic area. The objective study was investigate susceptibility gene M. isolates from individuals living a highly endemic Therefore we analyzed drug multidrug (MDR) patients Rio de Janeiro, Brazil. From total...
We carried out a cross-sectional study from January to December 2015 on 1,425 inhabitants floating population in the Brazilian Amazon (Murinin district, Pará State) describe population-based prevalence of tuberculosis (TB) 2011 2014, recent TB contacts (rCts) latently infected with Mycobacterium (LTBI) , coverage local health network, socio-environmental factors, and frequency intestinal parasitic infection (IPI). found that sanitary structure was inadequate, latrines being shared other...
The aim of this study was to determine the prevalence non-tuberculous mycobacteria (NTM) isolates at University Hospital, Reference Center for Aids in Rio de Janeiro, Brazil, during one year. We used standard biochemical tests species identification and IS1245 PCR amplification applied as a Mycobacterium avium specific marker. Four hundred four specimens from 233 patients yielded acid-fast bacilli growth. M. tuberculosis identified 85% NTM 15%. disseminated infection common event correlated...
The diagnosis of pleural tuberculosis (pTB) is difficult, and more sensitive specific techniques are needed. In the period August 1998 to November 2002, we evaluated 132 patients with a effusion submitted thoracentesis biopsy in tertiary care hospital Rio de Janeiro, Brazil. Three tests were performed compared fluid: ADA activity measurement, IgA‐ELISA for two combined Mycobacterium antigens, polymerase chain reaction (PCR) detection M. DNA. Ninety‐five (72%) given final pTB. Overall...
Human isolates of Mycobacterium collected in 16 different states Brazil were submitted to PCR-restriction analysis (PRA) a 439-bp fragment the hsp65 gene with HaeIII and BstEII. Fourteen allelic variants not described clinical so far observed among 36 (10%) 356 Brazilian strains, including new pattern for scrofulaceum, M. intracellulare, flavescens, two patterns fortuitum, three each gordonae terrae, one avium complex-like strains. Two unidentified strains also presented pattern, strongly...
Little is known regarding the restoration of specific immune response after combined antiretroviral therapy (cART) and anti-tuberculosis (TB) introduction among TB-HIV patients. In this study, we examined patients to Mycobacterium tuberculosis (Mtb) antigens evaluate dynamics different over time. Moreover, also evaluated influence two doses efavirenz factors associated with reconstitution.This a longitudinal study nested in clinical trial, where cART was initiated during baseline visit (D0),...
Isolates of Mycobacterium tuberculosis from 120 patients seen in the 12 months ending September 1994 at 2 tertiary-care centres Rio de Janeiro were characterized by IS6110 restriction fragment length polymorphism (RFLP) analysis. Ninety-seven (81%) had isolates with unique RFLP patterns, while 23 (19%) that belonged to 11 different cluster patterns. The strains latter distributed among 1 group 3 and 10 groups each. cluster-pattern not associated gender, age, HIV infection, type residence,...
The development of fast, inexpensive, and reliable tests to identify nontuberculous mycobacteria (NTM) is needed. Studies have indicated that the conventional identification procedures, including biochemical assays, are imprecise. This study evaluated a proposed alternative method in which 83 NTM isolates, previously identified by testing in-house M. avium IS1245-PCR amplification, were submitted following tests: thin-layer chromatography (TLC) mycolic acids PCR-restriction enzyme analysis...
For the first time in Brazil it was investigated occurrence of IgM anti-PGL-1 sera household contacts leprosy patients using ELISA methodology. The multibacillary showed significantly more immuno-reactivity than from paucibacillary patients. It observed a high subclinical infection incidence among (19.4%). percentage development 5% (1/21) seropositive contact group. This finding suggests that serology could be useful as prognostic test, but for better definition is necessary to test...
Abstract Several antigens of Mycobacterium tuberculosis have been identified and specificity to one or multiple could determine the distinction between protective pathogenic host reaction. Therefore T cell immune response combinations 38 kDa/CFP‐10, kDa/MPT‐64, ESAT‐6/MPT‐64 ESAT‐6/CFP‐10 (each related a single protein ) in individuals from endemic areas examined. ELISA was used detect IFN‐γ production PBMC priming with proteins panel 105 individuals: patients (6 untreated 32 treated) 67...
This study aimed to assess drug resistance in Mycobacterium tuberculosis strains isolated from sputum samples. To that end, samples were collected 263 patients suspected of having tuberculosis. All subjects lived the Complexo de Manguinhos, which is located city Rio Janeiro, Brazil. Cultures testing positive between October 2000 and December 2002 tested determine strain susceptibility isoniazid, rifampicin, streptomycin, ETHionamide, ETHambutol. Of 75 diagnosed with tuberculosis, at least...
SUMMARY Aimed at assessing the circulation of Mycobacterium tuberculosis in a highly endemic prison, this 13-month prospective study was performed on strains isolated from (TB) cases detected passively and actively. We used X-ray screening newly admitted inmates mass beginning again 1 year later. Of 94 genotyped by restriction fragment-length polymorphism, 79 (84·0%) belonged to one 12 identified clusters (2–21 each), including two main (18 21 cases, respectively). A history TB treatment...
Pleural tuberculosis (PL-TB) remains difficult to diagnose. An enzyme-linked immunosorbent assay (ELISA) was developed based on a construction containing the fusion of Rv3019c (MT10.3) and Rv1980c (MPT64) gene sequences, its performance evaluated in an area where TB is endemic. A total 92 pleural fluid (PF) samples at serial dilutions 1:50 1:800 were included ELISA IgA MT10.3-MPT64 evaluation: 70 from patients 22 with other pleurisies. Confirmation expression subsequent purification protein...
The PstS1 antigen is highly immunogenic, principally when combined with CFP10 during both latent and active TB infection. In the present study, a selected pstS1 gene fragment was cloned, fused CFP10, expressed in Escherichia coli. product [PstS-1(285-374):CFP10] compared to recombinant RD1 (region of deletion 1) protein (ESAT-6:CFP10) detecting Mycobacterium tuberculosis infection 108 recent contacts pulmonary (TB) cases, considering positive tuberculin skin test (TST) be baseline. release...
To describe some aspects of tuberculosis in a low-income community (the Complexo de Manguinhos, Rio Janeiro, Brazil), retrospective study was carried out. Of the 290 cases reported 2000-2002 period, 75.8% were new cases. The annual incidence rates 157/100,000 (2000), 205/100,000 (2001), and 145/100,000 (2002). Although there tendency toward decrease number over period studied, difference not significant, suggesting that continues to be endemic area. Therefore, despite existence local public...
Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates a woman with unknown HIV status, had been identified as belonging to Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such PCR-restriction enzyme (PRA) hsp65 gene, commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing 16S-23S internal transcribed spacer (ITS), presence lentiflavum was...