A5050156495- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Liver physiology and pathology
- Extracellular vesicles in disease
- Circular RNAs in diseases
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Hepatitis C virus research
- Phagocytosis and Immune Regulation
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Hepatitis B Virus Studies
- Adipose Tissue and Metabolism
- Cytokine Signaling Pathways and Interactions
- Mitochondrial Function and Pathology
- Mesenchymal stem cell research
- Organ Transplantation Techniques and Outcomes
- Occupational and environmental lung diseases
- Immune cells in cancer
- Renal Transplantation Outcomes and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- NF-κB Signaling Pathways
The First Hospital of Kunming
2021-2023
Kunming Medical University
2015-2023
First Affiliated Hospital of Kunming Medical University
2017
Abstract Background Circular RNAs (circRNAs) can be encapsulated into exosomes to participate in intercellular communication, affecting the malignant progression of a variety tumors. Dysfunction CD8 + T cells is main factor immune escape from hepatocellular carcinoma (HCC). Nevertheless, effect exosome-derived circRNAs on T-cell dysfunction needs further exploration. Methods The circCCAR1 tumorigenesis and metastasis HCC was assessed by vitro vivo functional experiments. function measured...
Background: Most patients with hepatitis B virus (HBV) infection will develop hepatocellular carcinoma (HCC). This study aimed to explore the potential mechanism of miR-142-3p in HCC caused by HBV infection. Methods: HepG2 cells and M1 macrophages were cocultured then infected establish an vitro model. MicroRNA (miRNA) messenger RNA (mRNA) expression was analyzed quantitative reverse transcription polymerase chain reaction (RT-qPCR) Western blot. The protein expressions COX2, ACSL4, PTGS2,...
Hepatocellular carcinoma (HCC) is one of the most prevalent cancers, and its incidence rate increasing worldwide. At present, there no ideal treatment for HCC. In recent years, molecular-targeted therapy has shown significant therapeutic benefits patients. Ferroptosis a modality regulated cell death, previous studies have found that inducing ferroptosis in liver cancer cells can inhibit progression cancer. The aim this study to investigate regulatory mechanism miR-21-5p regulating HCC cells....
We performed this study to determine the role of IL-17 in immune microenvironment hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC).HepG2 cells were treated with IL-17, STAT3 inhibitor S31-201 or IL-6 neutralizing monoclonal antibody (IL-6 mAb). Cell proliferation and migration compared using Counting kit-8 (CCK-8) Transwell assays, respectively. Real-time quantitative PCR (RT-qPCR), Western Blot, ELISA, immunofluorescence histological staining used for determining expression...
Hepatocellular carcinoma (HCC) cell-derived exosomes have shown effects on inducing M2 macrophage polarization and promoting HCC progression. MiR-452-5p was reported by recent studies to promote malignancy progression as an exosomal microRNA that secreted cells, of which the underlying mechanism remains unclear. Here, we further explored how miR-452-5p functions in HCC. expressions cells examined situ hybridization. Next, cell lines were transfected with mimics or inhibitor miR-452-5p....
Abstract Background Hepatocellular carcinoma (HCC) is a common cancer worldwide, and sorafenib first-line drug for the treatment of advanced liver cancer. Resistance to has become major challenge in hepatocellular carcinoma, however, studies have shown that metformin can promote ferroptosis sensitivity. Therefore, aim this study was investigate promotion sensitivity by via ATF4/STAT3 cells. Methods cells Huh7 Hep3B induced resistance (SR) Huh7/SR Hep3B/SR were used as vitro cell models....
Exosomes are becoming an important mediator of the interaction between tumor cells and microenvironment. Ferroptosis is a newly discovered type cell death. However, its role in progression liver cancer largely unknown. The aim presents study was to analyze mechanism by which hepatitis B virus (HBV)-positive secretes exosomes mediate iron death M1 macrophages, thereby promoting development cancer.Liver tissues peripheral blood with positive negative clinical HBV infection were collected,...
To validate the mechanism by which miR-21-5p mediates autophagy in drug-resistant cells hepatocellular carcinoma (HCC), aggravating sorafenib resistance and progression of HCC.HCC were treated with to establish sorafenib-resistant cells, nude mice subcutaneously injected hepatoma animal models. RT-qPCR was used determine level miR-21-5p, Western blotting related proteins. Cell apoptosis, cell migration, LC3 accessed. Immunohistochemical staining for detection Ki-67 LC3. A dual-luciferase...
Aerobic exercise has shown beneficial effects in the prevention and treatment of non-alcoholic fatty liver disease (NAFLD). Nevertheless, regulatory mechanism is not turely clear. Therefore, we aim to clarify possible by investigating aerobic on NAFLD its mitochondrial dysfunction.NAFLD rat model was established feeding high fat diet. used oleic acid (OA) treat HepG2 cells. Changes histopathology, lipid accumulation, apoptosis, body weight, biochemical parameters were assessed. In addition,...
Abstract Background In this study, the role of autophagy in hepatic fibrosis and its effects on macrophage polarization exosomes (EVs) were verified by establishing model co‐culture model, providing evidence for treatment. Methods CCL 4 was used to establish model. The morphology purity transmission electron microscopy, western blotting (WB), nanoparticle tracing analysis (NTA). Real‐time quantitative PCR (qRT‐PCR), WB enzyme‐linked immunoadsorption (ELISA) detect markers, markers liver...
Abstract Background This study aims to explore the effect of liver stem cells (LSCs)‐derived exosomes and miR‐142a‐5p carried by them on process fibrosis regulating macrophages polarization. Methods In this study, CCL 4 was used establish model. The morphology purity (EVs) were verified transmission electron microscopy, western blotting (WB) nanoparticle tracing analysis (NTA). Real‐time quantitative PCR (qRT‐PCR), WB enzyme‐linked immunoadsorption (ELISA) detect markers, macrophage...
Liver fibrosis is a major characteristic of most chronic liver diseases which leads to accumulation extracellular matrix (ECM) proteins. Hedgehog (Hh) pathway activated by Gli genes participated in the pathogenesis fibrosis. However, regulatory role miR-125b via targeting remains unknown.RT-qPCR and western blot were employed expression levels mRNA protein, respectively. The level tissue was determined Masson's trichrome staining. interaction between Gli3 tested luciferase reporter assay. In...
Liver transplantation significantly increases recurrence of hepatitis B virus (HBV) among high-risk patients. Hepatitis immunoglobulin (HBIG) and antiviral nucleotide analogues are effective prophylaxis reagents in preventing HBV recurrence. However, still occurs with these treatments.To explore a more cost-effective protocol patients after liver transplantation, we treated an initial high dose 10 000 IU HBIG during the anhepatic phase second at optimal time point surgery. The were...
Kidney transplantation is the promising treatment of choice for chronic kidney disease and end-stage can effectively improve quality life survival rates patients. However, allograft rejection following has a negative impact on transplant success. Therefore, present study aimed at screening novel biomarkers diagnosis improving long-term outcome. In study, total 8 modules 3065 genes were identified by WGCNA based GSE46474 GSE15296 dataset from Gene Expression Omnibus (GEO) database. Moreover,...
Acute liver failure is one of the most intractable clinical problems. The use bioartificial livers may solve donor shortage Human umbilical cord mesenchymal stem cells (hUCMSCs) are an excellent seed cell choice for artificial because they change their characteristics to resemble hepatocyte-like (HLCs) following transplantation.This study aimed determine whether immunological hUCMSCs changed after being transformed into cells.HUCMSCs were isolated by adherent method. hUCMSC surface markers...