A5100361127- Organ Transplantation Techniques and Outcomes
- Liver physiology and pathology
- Organ Donation and Transplantation
- Mesenchymal stem cell research
- Innovations in Medical Education
- Pediatric Hepatobiliary Diseases and Treatments
- Liver Disease and Transplantation
- Tissue Engineering and Regenerative Medicine
- Knee injuries and reconstruction techniques
- Gallbladder and Bile Duct Disorders
- Corneal surgery and disorders
- Pancreatitis Pathology and Treatment
- Organ and Tissue Transplantation Research
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Palliative Care and End-of-Life Issues
- Immune cells in cancer
- Mathematical and Theoretical Epidemiology and Ecology Models
- Shoulder Injury and Treatment
- Ocular Surface and Contact Lens
- Extracellular vesicles in disease
- Mechanisms of cancer metastasis
- Consumer Attitudes and Food Labeling
- Bioactive natural compounds
- Xenotransplantation and immune response
Kunming Medical University
2013-2024
First Affiliated Hospital of Kunming Medical University
2015-2024
Jinan Central Hospital
2024
Shandong University
2024
Peking University
2007-2024
Peking University Cancer Hospital
2024
Hangzhou Normal University
2024
The First Hospital of Kunming
2009-2023
Chinese Academy of Inspection and Quarantine
2022
Yunnan University
2021
Abstract Objective Premature ovarian insufficiency (POI) is a refractory disease that seriously affects female fertility. Growing body of evidence has indicated mesenchymal stem cells (MSCs) as promising resources in regenerative medicine. In this study, we treated POI patients with umbilical cord‐derived MSCs (UCMSCs) and then investigated the restoration function clinical outcomes through follow‐ups. Materials methods Sixty‐one diagnosed participated study. UCMSCs were isolated cultured...
Stem cell treatment for critical limb ischemia yields a limited therapeutic effect due to loss and dysfunction caused by local ischemic environment. Biomimetic scaffolds emerge as ideal delivery vehicles regulating fate via mimicking the components of stem niche. Herein, we prepared bioactive hydrogel mixing chitosan hyaluronic acid that is immobilized with C domain peptide insulin-like growth factor 1 (IGF-1C) examined whether this could augment survival potential. Our results showed...
Objective Systemic lupus erythematosus (SLE) is an autoimmune disease with complex etiology that not yet entirely understood. We aimed to elucidate the mechanisms and therapeutic potential of microRNAs (miRNAs) in SLE a Tibetan population. Methods Peripheral blood mononuclear cells from patients (n = 5) healthy controls were used for miRNA–mRNA co-sequencing detect miRNAs related immune abnormalities associated SLE. Luciferase reporter assay was identify targets candidate miRNA. The target...
Abstract Background This study aims to explore the effect of liver stem cells (LSCs)‐derived exosomes and miR‐142a‐5p carried by them on process fibrosis regulating macrophages polarization. Methods In this study, CCL 4 was used establish model. The morphology purity (EVs) were verified transmission electron microscopy, western blotting (WB) nanoparticle tracing analysis (NTA). Real‐time quantitative PCR (qRT‐PCR), WB enzyme‐linked immunoadsorption (ELISA) detect markers, macrophage...
The objective of this study was to investigate the protective effect notoginsenoside R1 (NGR1) on cerebral ischemia-reperfusion injury (CIRI) in rats, and its molecular mechanism, provide new insights into diagnosis treatment CIRI. Sixty Sprague-Dawley rats were randomly divided four groups including sham-operation group (Sham), model (CIR), NGR1 (NGR1), nimodipine positive control (NDC) with 15 each. Bilateral common carotid arteries occlusion used establish rat CIRI model. area infarction...
Transplanting a vascularized autologous submandibular gland (SMG) is considered an effective method to treat severe keratoconjunctivitis sicca. But the operation may fail due anatomic variances in blood vessels of SMG. The present study aimed investigate glands at microanatomy level.The including arteries and veins was investigated using 30 adult corpses 60 were anatomized under surgical microscope. lengths diameters arterial venous glandular branches measured sliding caliper.The mainly...
Extracorporeal cardiac shock wave (SW) therapy is an effective, safe, and non-invasive therapeutic strategy for severe coronary artery disease.Shock might affect tissues because of its ability to promote angiogenesis.In this report, we investigated if the up-regulation vascular endothelial growth factor (VEGF) by SW involved in cell proliferation cultured cells.After human umbilical vein cells were treated with SW, expression secretion VEGF as well analyzed.We also determined mechanism...
Background An apnea test is essential in the clinical determination of brain death. This study was conducted to analyse complications associated with Methods On 93 adult patients coma Zhejiang Province China from January 2003 December 2006, 179 tests were performed as a part Potential risk conditions and analysed during tests. Results During apnea, serious cardiac arrhythmia did not occur all patients. Complications occurred 37 (21%) Hypotension 30 (17%) it observed 8/94 (9%) baseline value...
Food additives play an important role in the food supply, and it has been a safety topic of great concern to public. There no systematic research on Chinese consumers' concerns, attitudes, feelings, or opinions supervision media coverage past decade, which is area worth exploring. This study was carried out deeply understand cognition formulate risk communication strategies China. Big data online public opinion China from 2011 2020 collected cleaned up using Haina Network Public Opinion...
To summarize the early postoperative pain management strategies for anterior cruciate ligament reconstruction (ACLR), and to select a reasonable effective plan promote functional rehabilitation after ACLR.
Despite progress in the field of immunosuppression, acute rejection is still a common postoperative complication following liver transplantation. This study aims to investigate capacity human hepatocyte growth factor (hHGF) modifying hepatic oval cells (HOCs) administered simultaneously with orthotopic transplantation as means improving graft survival. HOCs were activated and isolated using modified 2-acetylaminofluorene/partial hepatectomy (2-AAF/PH) model male Lewis rats. A HOC line stably...
Liver fibrosis is a major characteristic of most chronic liver diseases which leads to accumulation extracellular matrix (ECM) proteins. Hedgehog (Hh) pathway activated by Gli genes participated in the pathogenesis fibrosis. However, regulatory role miR-125b via targeting remains unknown.RT-qPCR and western blot were employed expression levels mRNA protein, respectively. The level tissue was determined Masson's trichrome staining. interaction between Gli3 tested luciferase reporter assay. In...
Severe acute pancreatitis (SAP) is a serious systemic disease with high mortality. Ulinastatin drug widely used for patients SAP and multiple organ failure syndrome. The present study aimed to investigate the capacity of serum C-reactive protein (CRP) levels predict therapeutic effects ulinastatin combined somatostatin as well determine severity SAP. were treated CRP measured. computed tomography index (CTSI), physiology chronic health evaluation II (APACHE II) gastrointestinal scores...
Glioblastoma (GBM) is one of the most aggressive forms cancer. Although IDH1 mutation indicates a good prognosis and potential target for treatment, GBMs are wild-type. Identifying additional molecular markers would help to generate personalized therapies improve patient outcomes. Here, we used our recently developed metabolic modeling method (genome-wide precision modeling, GPMM) investigate profiles GBM, aiming identify novel this disease. We systematically analyzed reaction 149 GBM...
Hepatocyte growth factor (HGF) is a multifunctional that controls cell scattering. It has been suggested it regulates the proliferation of hepatic oval cells (HOCs). Using HOC line stably expresses human HGF gene (hHGF), we investigated in vitro and differentiation characteristics hHGF-modified HOCs explored their potential capacity for intrahepatic transplantation. A modified 2-acetylaminofluorene partial hepatectomy (2-AAF/PH) model was established to activate rat liver. were transfected...
Background: To explore the impact of use vasoactive drugs in donation after cardiac death (DCD) donors on graft function, with an attempt to guide clinical practices organ preservation and DCD kidney transplantation.
GLI family zinc finger 3 (Gli3), as the upstream transcriptional activator of hedgehog signaling, has previously been demonstrated to participate in process liver fibrosis. The present study aimed investigate potential functions microRNA (miR)‑200a and Gli3 progression expression levels miR‑200a cells tissues were determined by PCR western blotting; interaction was evaluated bioinformatics analysis dual‑luciferase reporter assay. significantly reduced serum samples from clinical patients, a...
The combination of anti-angiogenesis agents with immune-checkpoint inhibitors is a promising treatment for patients advanced hepatocellular carcinoma (HCC); however, therapeutic resistance caused by cancer stem cells present in tumor microenvironments remains to be overcome. In this study, we report the first time that Kringle 1 domain human hepatocyte growth-factor α chain (HGFK1), previously described peptide, repressed sub-population CD90+ (CSCs) and promoted their differentiation...