A5030493825- melanin and skin pigmentation
- Animal Genetics and Reproduction
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Hair Growth and Disorders
- Renin-Angiotensin System Studies
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Reproductive Biology and Fertility
- Digestive system and related health
- Melanoma and MAPK Pathways
- Fire effects on ecosystems
- Genetic Mapping and Diversity in Plants and Animals
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- Gastrointestinal motility and disorders
- Genetic Syndromes and Imprinting
- Developmental Biology and Gene Regulation
- RNA regulation and disease
- Mast cells and histamine
- Advanced Proteomics Techniques and Applications
- Skin Protection and Aging
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Centre de Gestion Scientifique
2017-2021
Centre National de la Recherche Scientifique
2011-2021
Institut Pasteur
2009-2020
Department of Genomes & Genetics
2019
École Nationale Vétérinaire d'Alfort
2003-2017
Biologie du Développement et Cellules Souches
2017
Institut National de la Recherche Agronomique
1999-2015
Laboratoire de Génétique Cellulaire
2001-2012
Laboratoire de Recherche Scientifique
2010-2012
Université Paris-Est Créteil
2012
ABSTRACT In the mouse, Kit receptor and its ligand, stem cell factor (SCF), are encoded at W/Kit Steel loci, respectively. The Kit/SCF transduction pathway is involved in promoting cellular migration, proliferation and/or survival of melanoblasts, hematopoietic progenitors primordial germ cells. Furthermore, a functional required for development interstitial cells Cajal (ICC) small intestine. Whereas all c-kit-expressing embryogenesis were not identified, previous studies clearly...
The inner cell mass (ICM) of the implanting mammalian blastocyst comprises two lineages: pluripotent epiblast (EPI) and primitive endoderm (PrE). We have identified platelet-derived growth factor receptor alpha (PDGFRα) as an early marker PrE lineage its derivatives in both mouse embryos ex vivo paradigms extra-embryonic (ExEn). By combining live imaging embryo-derived stem cells expressing a histone H2B-GFP fusion reporter under control Pdgfra regulatory elements with analysis...
ABSTRACT Genetic and cell culture analyses have shown that the development of melanocytes from neural crest-derived precursor cells critically depends on tyrosine kinase receptor KIT basic-helix-loop-helix-leucine zipper transcription factor MITF. MITF show complex interactions in is needed for maintenance Kit expression melanoblasts signaling modulates activity stability melanocyte lines. Using primary crest cultures embryos homozygous a null allele marked by an inserted LacZ gene...
Human centronuclear and myotubular myopathies belong to a genetically heterogeneous nosological group with clinical variability ranging from fatal disorder mild weakness. The severe X-linked form is attributed more than 200 different mutations in the myotubularin encoding gene (MTM1). In contrast, there are no reports regarding molecular etiology or linkage studies on autosomal forms of disease. Labrador retrievers affected by spontaneous myopathy (cnm) have histological features human...
Rift Valley fever virus (RVFV) is an enzootic circulating in Africa that transmitted to its vertebrate host by a mosquito vector and causes severe clinical manifestations humans ruminants. RVFV has tripartite genome of negative or ambisense polarity. The M segment contains five in-frame AUG codons are alternatively used for the synthesis two major structural glycoproteins, GN GC, at least accessory proteins, NSm, 14-kDa cytosolic protein, P78/NSm-GN, 78-kDa glycoprotein. To determine...
In the mouse, more than 16 loci are associated with mutant phenotypes that include defective pigmentation, aberrant targeting of lysosomal enzymes, prolonged bleeding, and immunodeficiency, result biogenesis cytoplasmic organelles: melanosomes, lysosomes, various storage granules. Many these mouse mutants homologous to human Hermansky–Pudlak syndrome (HPS), Chediak–Higashi syndrome, Griscelli syndrome. We have mapped positionally cloned one loci, buff ( bf ), which has a phenotype similar...
Neural crest (NC) cells arise in the dorsal neural tube (NT) and migrate into embryo to develop many different cell types. A major unresolved question is when how fate of NC decided. There widespread evidence for multipotential cells, whose fates are decided during or after migration. also some that already divided subpopulations discrete precursors within NT. We have investigated this mouse embryo. find a subpopulation on most dorsomedial aspect NT express receptor tyrosine kinase Kit...
Neuronal ceroid lipofuscinoses (NCLs) represent the most common group of inherited progressive encephalopathies in children. They are characterized by loss vision, mental and motor deterioration, epileptic seizures, premature death. Rare adult forms NCL with late onset known as Kufs’ disease. Loci underlying these remain unknown due to small number patients genetic heterogeneity. Here we confirm that a late-onset form recessively segregates US French pedigrees American Staffordshire Terrier...
We have studied human renin messenger RNA by hybridization with the mouse submaxillary gland (SMG) cDNA probe. The kidney is about 1.6 kilobase (kb) long, similarly to SMG mRNA. A clone of 1.1 kb length was obtained. comparison nucleotide sequences and clones reveals conservation residues involved in catalytic mechanisms a potential glyco-sylation site. molecular probe allowed us study expression chorionic tissue. RNAs are similar length. Southern blot analysis presence single gene DNA.
The expression of the renin gene in rat kidneys was studied using mouse submaxillary gland complementary DNA. length messenger RNA (mRNA) approximately 1600 nucleotides, similar to that and kidney mRNA. Rat mRNA quantified by a radiodensitometric DNA hybridization assay. effects intense long-term stimulation short-term inhibition secretion on plasma concentration, renal content were compared with those controls. After 15 days sodium depletion captopril treatment, concentration increased...
Background Rift Valley fever virus (RVFV) causes disease in livestock and humans. It can be transmitted by mosquitoes, inhalation or physical contact with the body fluids of infected animals. Severe clinical cases are characterized acute hepatitis hemorrhage, meningoencephalitis and/or retinitis. The dynamics RVFV infection cell types vivo poorly understood. Methodology/Principal Findings strains expressing humanized Renilla luciferase (hRLuc) green fluorescent protein (GFP) were generated...
The mRNA encoding mouse renin has been partially purified from total poly(A)-containing RNA of submaxillary glands male Swiss mice. Corresponding cDNAs were cloned in the Pst I site pBR322. Recombinants have characterized by differential screening and hybrid-arrested translation. DNA clone pRn3-5 used to study expression gland kidney different strains. same length (1600 nucleotides) appear be products gene. In vitro translation mRNAs blotting experiments shown that sequences are accumulated...
Rift Valley fever (RVF) is an arthropod-borne viral disease repeatedly reported in many African countries and, more recently, Saudi Arabia and Yemen. RVF virus (RVFV) primarily infects domesticated ruminants, resulting miscarriage pregnant females death for newborns young animals. It also has the ability to infect humans, causing a feverish syndrome, meningoencephalitis, or hemorrhagic fever. The various outcomes of RVFV infection animals humans argue existence host genetic determinants...
Rift Valley fever virus (RVFV) is an emerging viral zoonosis that responsible for devastating outbreaks among livestock and capable of causing potentially fatal disease in humans. Studies have shown upon infection, certain viruses the capability utilizing particular cellular signaling pathways to propagate infection. Activation p53 important DNA damage cascade, initiation apoptosis, cell cycle arrest transcriptional regulation multiple genes. The current study focuses on role RVFV infection...
In recent outbreaks, ZIKV has infected millions of people and induced rare but potentially severe complications, including Guillain-Barré syndrome encephalitis in adults. While several viral sequence variants were proposed to enhance the pathogenicity ZIKV, influence host genetic mediating clinical heterogeneity remains mostly unexplored. We addressed this question using a mouse panel which models diversity human population strain from isolate. Through combination vitro vivo approaches, we...
The stem cell factor (SCF)-KIT signal transduction pathway plays a role in the proliferation, differentiation and survival of range progenitor types but little is known about its function embryonic (ES) cells. We generated ES cells carrying null allele Kit as well knock-in that encodes an SCF-independent hybrid KIT receptor can be activated by FKBP binding drug, AP20187. die when induced to differentiate upon withdrawal leukaemia inhibitory monolayer culture. This phenotype recapitulated...