The probiotic gut microbiome and its metabolites are pivotal in regulating host metabolism, inflammation, immunity. Host genetics, colonization at birth, the lifestyle, exposure to diseases drugs determine microbial composition. Dysbiosis disruption of homeostasis beneficial have been reported be involved tumorigenesis progression colorectal cancer (CRC). However, influence bacteria-secreted on CRC growth is yet fully elucidated. In this study, we compared composition patients healthy...

Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is a malignant form of cancer with the worst survival rate and an extremely low response to treatments. The development molecular characterization pancreatic cell lines (PCCLs) are essential for studying biology highly aggressive adenocarcinoma. Methods We applied whole exome sequencing (WES) RNA-seq identify characteristics 26 newly established PCCLs. Eighteen clinically relevant anti-cancer drugs were used assess heterogeneous...

// Sung-Hee Kim 1 , Soon-Chan 2 and Ja-Lok Ku 1, Laboratory of Cell Biology, Cancer Research Institute, Seoul National University College Medicine, 03080, Republic Korea Department Biomedical Sciences, Correspondence to: Ku, email: kujalok@snu.ac.kr Keywords: colorectal cancer, 5-Fu resistant-cell line, metformin, cancer stem cell (CSC), DNA replication Received: January 17, 2017 Accepted: April 27, Published: May 11, ABSTRACT Metformin is most widely prescribed for type diabetes. Recently,...

Abstract Intratumor heterogeneity (ITH) stands as one of the main difficulties in treatment colorectal cancer (CRC) it causes development resistant clones and leads to heterogeneous drug responses. Here, 12 sets patient‐derived organoids (PDOs) cell lines (PDCs) isolated from multiple regions single tumors patients, capturing ITH by multiregion sampling individual tumors, are presented. Whole‐exome sequencing RNA performed. The PDOs PDCs also analyzed with a clinically relevant 24‐compound...

Resistance to preoperative radiotherapy is a major clinical problem in the treatment for locally advanced rectal cancer. The role of NDRG1 resistance ionizing radiation cancer has not been fully elucidated. This study aimed investigate effect reduced intracellular expression on radio-sensitivity human cells exploring novel approaches cancer.Three radio-resistant cell lines (SNU-61R80Gy, SNU-283R80Gy, and SNU-503R80Gy) were established from (SNU-61, SNU-283, SNU-503) using total 80 Gy...

Peritoneal metastasis is one of the major patterns unresectability in colorectal cancer (CRC) and a cause death advanced CRC. Identification distinct gene expressions between primary CRC peritoneal seeding to predict metastatic potential human Three pairs (SNU-2335A, SNU-2404A, SNU-2414A) corresponding (SNU-2335D, SNU-2404B, SNU-2414B) cell lines were established determine different resulting aberrated signaling pathways tumor using whole exome sequencing microarray. Whole detected that...

Jee Hyung Lee, Haeryoung Kim, Sang Hyub Ja-Lok Ku, Jung Won Chun, Ha Young Seo, Soon Chan Woo Hyun Paik, Ji Kon Ryu, Kook Andrew M. Lowy, and Yong-Tae Kim. Gut Liver 2022;16:625-36. https://doi.org/10.5009/gnl210166

Organoid culture technique has been taking center stage as a next-generation ex-vivo model due to advancement of stem cell research techniques. The importance the laboratory-based ex vivo increasingly recognized for recapitulating histological, and physioglocal conditions in microenviorment. Accordingly, use this also broadened understanding intratumoral heterogeneity which is closely associated with varied drug responses observed patients. Likewise, studies on within single tumor tissue...

Abstract Purpose Early dissemination of primary pancreatic ductal adenocarcinoma (PDAC) is the main cause dismal prognosis as it highly limits possible treatment options. A number PDAC patients experience distant metastasis even after due to metastatic clones. We aimed demonstrate molecular architecture borderline resectable manifests cancer PDAC. Methods Here, 36 organoids isolated from tumor masses with diverse statues are presented. Whole-exome sequencing and RNA were performed drug...

Glioblastoma (GBM) is the most lethal intracranial tumor. Sequencing technologies have supported personalized therapy for precise diagnosis and optimal treatment of GBM by revealing clinically actionable molecular characteristics. Although accumulating sequence data from brain tumors matched normal tissues facilitated a comprehensive understanding genomic features GBM, these in silico evaluations could gain more biological credibility when they are verified with vitro vivo models. From this...

Abstract Colorectal cancer (CRC) represents the third most frequently diagnosed malignancy worldwide and is second common cause of tumor-associated mortalities in Korea. Due to disease’s aggressive behavior, 5-year survival rate for CRC patients remains unpromising. Well-characterized cell lines have been used as a biological model studying biology developing novel therapeutics. To assist vitro studies, 18 (SNU-1566, SNU-1983, SNU-2172, SNU-2297, SNU-2303, SNU-2353B, SNU-2359, SNU-2373B,...

Colorectal cancer (CRC) is the third most common diagnosed worldwide and represents second cause of all cancer-related deaths in Korea. Although epidemiological data indicate a sharp increase incidence CRC among individuals older than 50 years, more 10% CRCs occur before reaching 50. These are known as early-onset (EOCRCs) likely to be suggestive hereditary predisposition. However, familial syndromes account for only 20% genetic aberrations EOCRC, remaining 80% still question. Therefore, we...

Abstract The clinical phenotype linked with mutations in ABCB1, encoding P‐glycoprotein, has never been reported. Here, we describe twin sisters biallelic ABCB1 who showed recurrent reversible encephalopathy accompanied by acute febrile or afebrile illness. Whole‐exome sequencing was performed on one of the and her healthy parents, revealed compound heterozygous loss‐of‐function variants ABCB1. patient brains displayed substantial loss xenobiotic clearance ability, as demonstrated [ 11...

Multifocal colorectal cancer (CRC) comprises both clonally independent primary tumors caused by inherited predisposition and related mainly due to intraluminal spreading along an intact basement membrane. The distinction between these multifocal CRCs is essential because therapeutic strategies vary according the clonal association of multiple tumor masses. Here, we report one unique case synchronous intestinal (SIC) with occurring entire bowel tract, including small intestine. We established...

Objective The incidence of pancreatic adenocarcinoma (PA) approximates its prevalence, as the malignancy is almost consistently fatal within a year. Although currently available adjuvant therapy seems to provide survival benefit, it only moderate, and standard regimen has not yet been established. Therefore, more biological resources investigate PA are needed. Methods Here, we established characterized 10 human cancer cell lines derived from primary tumor mass. Whole exome sequencing...

Abstract Malignant pleural effusion (MPE) is an independent determinant of poor prognostic factor non-small cell lung cancer (NSCLC). The course anchorage growth within the cavity likely reforms innate molecular characteristics malignant cells, which largely accounts for resistance to chemotherapy and prognosis after surgical resection. Nevertheless, genetic transcriptomic features with respect various drug responses MPE-complicated NSCLC remain poorly understood. To obtain a clearer...

<title>Abstract</title> The gut microbiome and its metabolites are pivotal for regulating host metabolism, inflammation, immunity. Host genetics, colonization at birth, the lifestyle, exposure to diseases drugs determine microbial composition. Dysbiosis disruption of homeostasis in have been reported be involved tumorigenesis progression colorectal cancer (CRC). However, influence bacteria-secreted on CRC growth is yet fully elucidated. In this study, we compared composition patients healthy...

Abstract (a) An introductory sentence indicating the purposes of study Distant metastasis is major cause cancer-related death in colorectal cancer (CRC) patients. Although Calponin family has emerged as a distinguishing feature epithelial-to-mesenchymal transition (EMT) human cancer, role CNN3 member metastatic CRC not been investigated. (b) A brief description pertinent experimental procedures. Three pairs primary and corresponding matched cell lines were analyzed for gene expression by...

Pancreatic ductal adenocarcinoma (PDAC) is known to be one of the most lethal cancers among all cancer types, with a relative 5-year survival rate less than 8%. Currently, surgery only probable curative treatment for PDAC which available 10-15% patients diagnosed cancer. Organoids resemble original tissue in morphology and function self-organizing capacity. can cultured high effectiveness from individual patient derived tumor makes them an extremely fitting model translational uses...

Abstract A number of genes have been studied in accordance with their metastatic potential. Nevertheless, widely used colorectal cancer cell lines such as SW-620 or IS3 only show the influence within limited mutational landscape. Here, three pairs primary CRC (SNU-2335A, SNU-2335D, SNU-2404A, SNU-2404B, SNU-2414A, and SNU-2414B) corresponding metastasis were established analyzed by whole exome sequencing microarray. Three SNU-2414A) matched (SNU-2335D, for gene expression microarray...