The debittering effect of extracellular enzymes from Bacillus subtilis ACCC 01746 was studied using soybean meal as a substrate for solid-state fermentation (SSF). Results showed that B. produces proteases and carboxypeptidase in the early stage SSF (0–8 h). Proteases are dominant can hydrolyze protein into long-chain peptides with mild bitterness. Carboxypeptidase production is at 8–16 h SSF, which point further hydrolyzed bitterness enhanced. strain then additional after 16 h, reduced. We...

An experiment was conducted to evaluate the effects of dietary acetyl-L-carnitine (ALC) on growth performance, carcass characteristics, meat quality and lipid metabolism in broilers.A total 240 one-day-old male Arbor Acres broilers were randomly allocated 4 treatments (0, 300, 600, 900 mg/kg ALC supplementation, respectively).Compared with control treatment, addition resulted lower (linear effect, p<0.05)ADG AFI.Abdominal fat percentage decreased p<0.05) as increased, but there no effect...

The knowledge on the dose-response relationships between cumulative phthalate exposure and reproductive hormones in human are lacking. To assess characteristics of associations hazard index (HI) di-n-butylphthalate (DBP) di-2-ethylhexylphthalate (DEHP) exposures serum concentrations free testosterone (fT), estradiol, luteinizing hormone (LH) follicle-stimulating (FSH), we used restricted cubic spline function to characterize curves HI values for 74 male workers occupationally exposed high...

Bacillus sp. H16v8 and HGD9229 were identified as Aflatoxin B1 (AFB1) degrader in nutrient broth after a 12 h incubation at 37 °C. The degradation efficiency of the two-strain supernatant on 100 μg/L AFB1 was higher than bacterial cells cell lysate. Moreover, degradations strongly affected by metal ions which Cu2+ stimulated Zn2+ inhibited degradation. extracellular detoxifying enzymes produced co-cultivation two strains isolated purified ultrafiltration. molecular weight range 20-25 kDa...

Fermented forages are important feedstuffs. Bacillus subtilis inoculants often used to improve the value of forage legume fermentation. The present work was conducted study effects replacing soybean meal with solid-state fermented alfalfa (FAM) B. ACCC 01746 on growth performance, serum antioxidant and digestive enzyme activities, cecal microflora in goose. 300 healthy geese similar body weights were randomly assigned six treatment groups five replicates 10 (five males females) each. Geese...

An experiment was conducted to evaluate the effects of dietary alpha-lipoic acid (LA) and acetyl-L-carnitine (ALC) on growth performance, carcass characteristics meat quality in Arbor Acres broilers. A total 486 1-d-old male broilers were randomly allocated 9 treatments, treatments group (0 mg/kg LA 0 ALC), B (50 C (100 D 50 E F G 100 H I ALC). Birds slaughtered at 42 days old. Average daily gain (ADG), average feed intake (AFI), conversion rate (FCR), eviscerated rate, breast muscle...

β-Conglycinin, the main component of 7S globulin in soybean protein, is also a key antigen protein that causes allergic reactions. Extrusion technologies have received considerable attention as method for modifying allergens. This study investigated changes β-conglycinin structure and antigenicity upon extrusion. Isoelectric precipitation, ammonium sulfate sepharose CL-6B gel filtration were used to isolate purify from powder, single-factor orthogonal tests effects water content, extrusion...

Due to its beneficial health effects, the use of soybean protein has shown a continuous increase, but concerns regarding allergenicity antigenic have also increased. This study aimed evaluate hydrolytic effects non-commercial alkaline protease isolated from Bacillus subtilis ACCC 01746 on β-conglycinin and hydrolysates. Alkaline strain was separated by precipitation method organic solvents, alkali-solution acid-isolation purified gel column. Using degree hydrolysis (DH) inhibition rate as...

Chicken coccidiosis is a common and severe parasitic disease caused by infection from Eimeria spp., which affects the integrity of intestinal mucosa. TGF-β has been shown to play an important role in healing mucosas, immunity, maintenance bowel mucosa integrity. Very little known about presence components TGF-β/Smads signaling pathway chicken at different times following coccidian infection. In present study, we measured levels TGF-β2, 3, 4, receptor TβRI, II, down-stream Smad 2, 7 cecum...

Abstract With brown adipose tissue (BAT) becoming a possible therapeutic target to counteract obesity, the prenatal environment could represent critical window modify BAT function and browning of white AT. We investigated if levels uncoupling protein 1 (UCP1) UCP1-mediated thermogenesis are altered in offspring exposed obesity. Female CD-1 mice were fed high-fat (HF) or standard-fat (SF) diet for 3 months before breeding. After weaning, all pups placed on SF. UCP1 mRNA quantified using...

β-Conglycinin, the main protein of soybean, is a key allergen that causes soybean allergies, and hydrolysis usually applied to lower its antigenicity. We evaluated enzymolysis characters β-conglycinin from perspective kinetics using alkaline protease B. subtilis ACCC 01746. A dynamic model describing was proposed initial substrate concentration, enzyme dosage (enzyme ratio) time as variables illustrate kinetic behavior enzymatic hydrolysis. The carried out at 60 g/L 0.6% dosage, 55 °C pH 8.5...

This experiment aimed to investigate whether Escherichia coli (E. coli) infection could affect the TGF-β/smads signaling pathway in jejunal tissue of chickens. One-day-old Cobb 500 broilers were randomly divided into 2 groups and treated with intraperitoneal E. or broth injection. Clinical signs birds assessed every day. Spleen bursa Fabricius birds, post-infection (pi), collected evaluate immune organ index. Jejunal tissues ascertain expression TGF-βs, TβRs, Smads. The results showed that...

The Er3+/Yb3+ co-doped Y4GeO8 crystal powders were successfully synthesized us-ing a high-temperature solid-phase method. structure of the obtained phosphors was confirmed to be pure through X-ray diffraction (XRD) analysis. A regression equation correlating doping concentrations with luminescent intensity estab-lished based on optimized theoretical model derived from experimental design. op-timal Er3+ and Yb3+ under 980 nm laser excitation determined as 7.41% 21.34%, respectively, while...

In this study, we revealed the differential proteins from wheat endosperms using proteomic analysis and investigated their surface properties. The pattern of polypeptides obtained Yangmai-15 Yangmai-16 varieties were compared two-dimensional polyacrylamide gels. addition, characteristics grain such as hardness, protein content, wet gluten, dough development time, stability, gliadin glutenin contents between Yangmai-16, results significantly different. Notably, 216 197 spots separated...

Abstract The purpose of this experiment was to study the effect adding selenized Bacillus subtilis peptidoglycan (Se-PG) in feed on growth performance, immunity, and antioxidant function carp ( Cyprinus carpio haematopterus ). Healthy were fed with five experimental diets supplemented 0 (control group), 50, 100, 200, 400 mg/kg Se-PG for 56 days. Each group had three replicates 30 fish per replicate. results as follows. weight gain rate specific significantly higher than those control P &lt;...

Effects of oxidized peptidoglycan (OPG) on immune and stress responses lipopolysaccharide (LPS)-induced damage in the liver carp were investigated this study. Four hundred carps (Cyprinus carpio haematopterus) fed with five experimental diets supplemented 0, 100, 200, 400, 800 mg kg-1 OPG for 28 days. Each group had four replicates 20 fish per replication. LPS challenge (injection 40 saline or LPS) occurred at day 29. The supplementation linearly increased (p&lt;0.05) plasma total protein,...