A5054290375- Immune Cell Function and Interaction
- Glutathione Transferases and Polymorphisms
- Mitochondrial Function and Pathology
- Eicosanoids and Hypertension Pharmacology
- Trypanosoma species research and implications
- Inflammasome and immune disorders
- Coenzyme Q10 studies and effects
- Biochemical Acid Research Studies
- Ubiquitin and proteasome pathways
- Adenosine and Purinergic Signaling
- Epigenetics and DNA Methylation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Fibroblast Growth Factor Research
- Inflammatory mediators and NSAID effects
- Microbial Metabolic Engineering and Bioproduction
- Immune Response and Inflammation
- Neonatal Respiratory Health Research
- Viral Infectious Diseases and Gene Expression in Insects
- Pharmacological Effects of Natural Compounds
- Biochemical and Molecular Research
- Asthma and respiratory diseases
- Fatty Acid Research and Health
- 3D Printing in Biomedical Research
- S100 Proteins and Annexins
- Immune cells in cancer
Trinity College Dublin
2016-2023
The University of Sydney
2017-2018
Insulin resistance in muscle, adipocytes and liver is a gateway to number of metabolic diseases. Here, we show selective deficiency mitochondrial coenzyme Q (CoQ) insulin-resistant adipose muscle tissue. This defect was observed range vitro insulin models tissue from humans concomitant with lower expression mevalonate/CoQ biosynthesis pathway proteins most models. Pharmacologic or genetic manipulations that decreased CoQ triggered oxidants while supplementation either cell mice restored...
Abstract Activated caspase-1 and caspase-11 induce inflammatory cell death in a process termed pyroptosis. Here we show that Prostaglandin E 2 (PGE ) inhibits caspase-11-dependent pyroptosis murine human macrophages. PGE suppreses expression macrophages the airways of mice with allergic inflammation. Remarkably, caspase-11-deficient are strongly resistant to developing experimental airway inflammation, where is known be protective. Expression elevated lung wild type Blocking production...
The NLRP3 inflammasome is a cytosolic complex sensing phagocytosed material and various damage-associated molecular patterns, triggering production of the pro-inflammatory cytokines interleukin-1 beta (IL)-1β IL-18 promoting pyroptosis. Here, we characterize glutathione transferase omega 1-1 (GSTO1-1), constitutive deglutathionylating enzyme, as regulator inflammasome. Using small molecule inhibitor GSTO1-1 termed C1-27, endogenous knockdown, GSTO1-1-/- mice, report that involved in...
Abstract PGs are important proinflammatory lipid mediators, the significance of which is highlighted by widespread and efficacious use nonsteroidal anti-inflammatory drugs in treatment inflammation. 4-Octyl itaconate (4-OI), a derivative Krebs cycle–derived metabolite itaconate, has recently garnered much interest as an agent. In this article, we show that 4-OI limits PG production murine macrophages stimulated with TLR1/2 ligand Pam3CSK4. This decrease secretion due to robust suppression...
Abstract The protozoan parasite Trypanosoma brucei is the causative agent of neglected tropical disease human African trypanosomiasis, otherwise known as sleeping sickness. Trypanosomes have evolved many immune-evasion mechanisms to facilitate their own survival, well prolonging host survival ensure completion parasitic life cycle. A key feature bloodstream form T. secretion aromatic keto acids, which are metabolized from tryptophan. In this study, we describe an immunomodulatory role for...
Research into cellular metabolism has become more high-throughput, with typical cell-culture experiments being performed in multiwell plates (microplates). This format presents a challenge when trying to collect gaseous products, such as carbon dioxide (CO2), which requires sealed environment and vessel separate from the biological sample. To address this limitation, we developed gas trapping protocol using perforated plastic lids plates. We used trap design measure CO2 production glucose...
Abstract Annexin A1 is a key anti-inflammatory effector protein that involved in the effects of glucocorticoids. 4-Octyl itaconate (4-OI), derivative endogenous metabolite itaconate, which abundantly produced by LPS-activated macrophages, has recently been identified as potent agent. The 4-OI share significant overlap with those dimethyl fumarate (DMF), derivate another Krebs cycle fumarate, already use clinically for treatment inflammatory diseases. In this study we show both and DMF induce...
The NLRP3 inflammasome is a cytosolic complex sensing phagocytosed material and various damage associated molecular patterns, triggering production of the proinflammatory cytokines IL-1β IL-18, promoting pyroptosis. Here, we characterise Glutathione transferase omega 1-1 (GSTO1-1), constitutive deglutathionylating enzyme, as novel regulator. Using small molecule inhibitor GSTO1-1 termed C1-27, endogenous knockdown GSTO1-1-/- mice, report that involved in activation. Mechanistically,...
Exploring the intricacies of host-pathogen communication is vital in order to understand why some microbes persist within a host, while others are effectively cleared. As such, an urgent area research underpinning human health and disease, especially given emerging concerns antibiotic resistance. Understanding pathways requires intricate knowledge fields both microbiology immunology. Living world specialists, where scientists sometimes invest their whole career studying one organism,...