A5035712288- Plant tissue culture and regeneration
- Cancer Treatment and Pharmacology
- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Fungal Biology and Applications
- Plant Molecular Biology Research
- Antimicrobial agents and applications
- Microtubule and mitosis dynamics
- Environmental Chemistry and Analysis
- Plant-Microbe Interactions and Immunity
- Lipid metabolism and biosynthesis
- Transgenic Plants and Applications
- Surfactants and Colloidal Systems
Universitat de Barcelona
2020-2023
Institute of Advanced Chemistry of Catalonia
2022
Engineered plant cell lines have the potential to achieve enhanced metabolite production rates, providing a high-yielding source of compounds interest. Improving taxanes, pharmacologically valuable secondary metabolites Taxus spp., is hindered by an incomplete knowledge taxane biosynthetic pathway. Of five unknown steps, three are thought involve cytochrome P450-like hydroxylases. In current work, after in-depth in silico characterization four candidate enzymes proposed previous cDNA-AFLP...
More knowledge is needed about the molecular/cellular control of paclitaxel (PTX) production in
Cationic surfactants have great potential as drug vehicles and for use in gene therapy (cationic vesicles made from cationic can encapsulate RNA or DNA cellular transfer). They also be used antimicrobial antifungal agents to treat human infections. In an era of increasing resistance, the development new biocompatible suitable application is high interest. this work, a library amino acid-based was synthesized, characterized tested activity. The head group architecture (number type acids,...
Elicited cell cultures of Taxus spp. are successfully used as sustainable biotechnological production systems the anticancer drug paclitaxel, but effect induced metabolomic changes on synthesis other bioactive compounds by elicitation has been scarcely studied. In this work, a powerful combinatorial approach based and untargeted metabolomics was applied to unravel characterize effects elicitors 1 µM coronatine (COR) or 150 salicylic acid (SA) phenolic biosynthesis in baccata suspensions....
Paclitaxel is one of the most effective anticancer drugs ever developed. Although sustainable approach to its production provided by plant cell cultures, yield limited bottleneck enzymes in taxane biosynthetic pathway: baccatin-aminophenylpropanoyl-13-O-transferase (BAPT) and 3'-N-debenzoyltaxol N-benzoyltransferase (DBTNBT). With aim enhancing paclitaxel overcoming this bottleneck, we obtained distinct lines Taxus baccata vitro roots, each independently overexpressing either two...
Taxus cell cultures are a reliable biotechnological source of the anticancer drug paclitaxel. However, interplay between taxane production and other metabolic pathways during elicitation remains poorly understood. In this study, we combined untargeted metabolomics elicited baccata to investigate variations in taxane-associated metabolism under influence 1 µM coronatine (COR) 150 salicylic acid (SA). Our results demonstrated pleiotropic effects induced by both COR SA elicitors, leading...