A5016208959- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Mathematical Biology Tumor Growth
- Cancer Cells and Metastasis
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Botanical Research and Applications
- Cell Image Analysis Techniques
- Phytase and its Applications
- Colorectal and Anal Carcinomas
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Atherosclerosis and Cardiovascular Diseases
Beckman Research Institute
2018-2021
City of Hope
2017-2021
City Of Hope National Medical Center
2018-2020
National Cancer Center
2018
Abstract Functional CD8 + T cells in human tumors play a clear role clinical prognosis and response to immunotherapeutic interventions. PD-1 expression involved chronic infections such as melanoma often correlates with state of T-cell exhaustion. Here we interrogate tumor-infiltrating lymphocytes (TILs) from breast explore their functional state. Despite exhaustion hallmarks, expression, tumor TILs retain robust capacity for production effector cytokines degranulation capacity. In contrast,...
CD8+ tumor-infiltrating lymphocytes (TILs) correlate with relapse-free survival (RFS) in most cancer types, including breast cancer. However, subset composition, functional status, and spatial location of TILs relation to RFS human tumors remain unclear. Spatial tissue analysis via quantitative immunofluorescence showed that infiltration T cells into islands was more significantly associated than cell either tumor stroma or total tumor. Localization within is mediated by expression the...
The prognostic value of immune cell infiltration within the tumor microenvironment (TME) has been extensively investigated via histological and genomic approaches. Based on positive T infiltration, Immunoscore developed validated for predicting risk recurrence colorectal cancer (CRC). Also, association between a consensus helper 1 (Th-1) response favorable clinical outcomes observed across multiple types. Here, we reanalyzed public data sets from Cancer Genome Atlas (TCGA) NCBI Gene...
BackgroundIt is increasingly recognized that cancer progression induces systemic immune changes in the host. Alterations number and function of cells have been identified patients' peripheral blood lymphoid organs. Recently, we found dysregulated cytokine signaling T from breast (BC) patients, even those with localized disease.MethodsWe used phosphoflow cytometry to determine clinical significance responsiveness monocytes non-metastatic BC patients at diagnosis. We also examined correlation...
Background: Microsatellite instability-high (MSI-H) and polymerase ε (POLE)-mutated metastatic colorectal cancer (mCRC) represent hypermutated ultramutated tumor phenotypes, respectively, that may predict benefit to checkpoint blockade [anti-programmed cell death 1 (PD-1)/programmed ligand (PD-L1)]. Methods: Immune profiling through multispectral fluorescent immunohistochemistry (IHC) using a multi-marker staining panel was performed on pretreatment specimens from cohort of MSI-H or...
Abstract While tumor infiltration by CD8 + T cells is now widely accepted to predict outcomes, the clinical significance of intratumoral B less clear. We hypothesized that spatial distribution rather than density within tumors may provide prognostic significance. developed statistical techniques (fractal dimension differences and a box-counting method ‘occupancy’) analyze tumor-infiltrating lymphocytes (TILs) in human triple-negative breast cancer (TNBC). Our results indicate good outcome...
The evolutionary changes in immune profiles of triple negative breast cancer (TNBC) are not well understood, although it is known that checkpoint inhibitors have diminished activity heavily pre-treated TNBC patients. This study was designed to characterize profile longitudinal tumor specimens by studying subsets infiltrating lymphocytes (TILs) paired primary and metastatic a cohort "poor outcome" (relapsed within 5 years) Immune TNBCs "good (no relapse patients were also analyzed....
The goal of immunotherapy is to mobilize the immune system kill cancer cells. Immunotherapy more effective and, in general, prognosis better, when cells infiltrate tumor. We explore question whether spatial distribution rather than just density tumor important forecasting recurs. After reviewing previous work on this issue, we introduce a novel application maximum entropy quantify discrete point-like objects. apply our approach B and T images tissue taken from triple negative breast...
Abstract CD8+ T cell infiltration into solid tumors has been shown to correlate with patient prognosis in numerous carcinomas. Furthermore, response checkpoint blockade therapies linked an induction of function and rescue exhaustion patients. Expression both PD-1 CD39 on exhausted cells described murine models chronic disease recently human Here we profile breast tumor, melanoma, lymphocytic leukemia (CLL) for the frequency phenotype cells. We find that is highest melanoma among these tumor...
Abstract While the density of tumor-infiltrating lymphocytes (TILs) is now well known to correlate with clinical outcome, significance spatial distribution TILs not characterized. We have developed novel statistical techniques (including fractal dimension differences, a hotspot analysis, box counting method that we call ‘occupancy’ and way normalize cell ‘thinning’) analyze (at different length scales) various types in triple negative breast tumors. Consistent prior reports, CD20 + B cells...
Abstract Background: Triple negative breast cancer (TNBC) is a heterogeneous disease with several molecular subtypes: basal-like1 (BL-1), basal-like 2 (BL-2), mesenchymal (M), and luminal androgen receptor (LAR). Molecular evolution of TNBC through chemotherapy selection pressure well recognized but poorly understood. In addition, approximately 20% TNBCs respond to PD-1 or PD-L1 inhibitors. It has been observed that heavily pre-treated patients may not immunotherapy. This study was carried...
The goal of immunotherapy is to enhance the ability immune system kill cancer cells. Immunotherapy more effective and, in general, prognosis better, when cells infiltrate tumor. We explore question whether spatial distribution rather than just density tumor important forecasting recurs. After reviewing previous work on this issue, we introduce a novel application maximum entropy quantify discrete point-like objects. apply our approach B and T images tissue taken from triple negative breast...