A5039962127- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Cancer, Stress, Anesthesia, and Immune Response
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Hepatocellular Carcinoma Treatment and Prognosis
- Long-Term Effects of COVID-19
- Cardiac tumors and thrombi
- T-cell and B-cell Immunology
- Genetic Neurodegenerative Diseases
- COVID-19 and healthcare impacts
- Vascular anomalies and interventions
- Coronary Artery Anomalies
- Healthcare Technology and Patient Monitoring
- Non-Invasive Vital Sign Monitoring
- Neurological diseases and metabolism
- Oropharyngeal Anatomy and Pathologies
- Immune cells in cancer
- Quality and Safety in Healthcare
- Central Venous Catheters and Hemodialysis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Telemedicine and Telehealth Implementation
- Nanoplatforms for cancer theranostics
- Advanced Breast Cancer Therapies
Albany Medical Center Hospital
2025
University of Washington
2025
The University of Texas Rio Grande Valley
2019-2024
Beckman Research Institute
2018-2024
City of Hope
2016-2024
Doctors Hospital at Renaissance
2019-2022
City Of Hope National Medical Center
2021
Discordance between endoscopic and histologic assessments in Crohn's disease (CD) have been observed, however the prevalence cause are unclear. To assess if a protocolized approach to biopsy collection facilitates understanding of this discordance patients with ileal CD. Patients known CD underwent colonoscopy activity assessment. Three biopsies were taken respectively from an ulcer edge, 7-mm, 14-mm away edge discrete ulcer(s). In no ulcers as controls, same 3-site protocol was applied...
Abstract Functional CD8 + T cells in human tumors play a clear role clinical prognosis and response to immunotherapeutic interventions. PD-1 expression involved chronic infections such as melanoma often correlates with state of T-cell exhaustion. Here we interrogate tumor-infiltrating lymphocytes (TILs) from breast explore their functional state. Despite exhaustion hallmarks, expression, tumor TILs retain robust capacity for production effector cytokines degranulation capacity. In contrast,...
CD8+ tumor-infiltrating lymphocytes (TILs) correlate with relapse-free survival (RFS) in most cancer types, including breast cancer. However, subset composition, functional status, and spatial location of TILs relation to RFS human tumors remain unclear. Spatial tissue analysis via quantitative immunofluorescence showed that infiltration T cells into islands was more significantly associated than cell either tumor stroma or total tumor. Localization within is mediated by expression the...
BackgroundIt is increasingly recognized that cancer progression induces systemic immune changes in the host. Alterations number and function of cells have been identified patients' peripheral blood lymphoid organs. Recently, we found dysregulated cytokine signaling T from breast (BC) patients, even those with localized disease.MethodsWe used phosphoflow cytometry to determine clinical significance responsiveness monocytes non-metastatic BC patients at diagnosis. We also examined correlation...
CD8+ tumor-infiltrating lymphocytes (TILs) are associated with improved survival in triple-negative breast cancer (TNBC) yet have no association estrogen receptor-positive (ER+) BC. The basis for these contrasting findings remains elusive. We identified subsets of BC tumors infiltrated by T cells characteristic features exhausted (TEX). Tumors abundant TEX exhibited a distinct tumor microenvironment marked amplified interferon-γ signaling-related pathways and higher programmed death ligand 1...
Abstract Trial Information Click here to access other published clinical trials. Lessons Learned Radioembolization with yttrium-90 resin microspheres can be combined safely full doses of durvalumab and tremelimumab in patients metastatic colorectal cancer. Regional radioembolization did not result any hepatic or extrahepatic responses a combination tremelimumab. The lack immunomodulatory on biopsies before after treatment rules out potential role for this strategy converting “cold tumor”...
Single-agent pembrolizumab treatment of hormone receptor-positive metastatic breast cancer (MBC) has demonstrated modest clinical responses. Little is known about potential biomarkers or mechanisms response to immune checkpoint inhibitors (ICIs) in patients with HR+ MBC. The present study presents novel correlates responses combined CDK4/6i and ICI. A analysis two independent phase I trials treating MBC was performed. Patients treated the combination palbociclib+the ICI pembrolizumab+the...
Abstract CD8+ Tumor Infiltrating Lymphocytes (TILs) have been shown to correlate with patient prognosis in breast cancer. Recently, resident memory T cells (TRM) established as a potent functional subset of that exist peripheral tissue without recirculation. We assayed from fresh tumors phenotype and found them be almost exclusively made up effector cells. Further profiling by examining expression CD103 CD69 showed on average 40% TILs are composed TRM. Functional analysis these robust...
<p>Immune composition differences between tumor-free and tumor-involved tissues. Matchstick plots depict p-values of significant A, bone B, brain C, skin lung tissues for all immune subsets densities frequencies assessed. Negative log10 transformed are displayed sorted from most to least within the Yellow lines indicate a greater density/frequency in tissue as compared tissues, while green Frequencies complex cell D, CD8+ T cells, E, CD4+ F, monocytes. G, HLA-DR low cells amongst...
<p>Statistical comparison of immune subset densities across metastatic and non-metastatic tissues. Densities cell subsets compared organ sites within A, general B, complex subsets. Statistics generated by Wilcoxon rank sum tests.</p>
<p>Evaluation of immune densities in segmented tissue areas across different organ sites. Densities phenotype cells within were compared tumor-involved sites as shown (A, B). Statistics generated by Kruskal–Wallis test ranks (A ) and Wilcoxon rank sum tests (B). Calculated p values are displayed *, < 0.05.</p>
<p>Detailed comparison of immune densities between metastatic and non-metastatic tissue samples the same organ type. Densities cell subsets within A, CD8+ T cells, B, CD4+ C, B D, NK E, DCs, F, myeloid cells in tumor-free (blue) tumor-involved (red) tissues assayed by flow cytometry. Statistics generated Wilcoxon rank sum tests. Calculated p values are displayed as *, < 0.05</p>
<p>Comparison of immune subset composition between organ sites. Frequencies complex cell subsets compared across tumor-free and tumor-involved sites within A, CD8+ T cells, B, CD4+ C, B D, NK E, monocytes. Statistics generated by Wilcoxon rank sum tests. Calculated p values are displayed as *, < 0.05; **, 0.01; ***, 0.001.</p>
<p>Paired analysis of immune densities between tumor-free and tumor-involved tissues. Densities cell subsets as determined by flow cytometry are plotted connecting matched (blue) (red) Statistics generated paired student t-tests. Calculated p values displayed.</p>
<p>Detailed comparison of immune densities between different organ sites metastatic or non-metastatic tissue. Densities cell subsets within A, CD8+ T cells, B, CD4+ C, B D, NK E, DCs, and F, myeloid cells in tumor-free (blue) tumor-involved (red) tissues assayed by flow cytometry. Statistics generated Kruskal–Wallis test ranks. Calculated p values are displayed as *, < 0.05; **, 0.01; ***, 0.001; ****, 0.0001.</p>
<p>PD-L1 expression assessment in metastatic tumor tissues. A, PD-L1 tumor-involved tissues as assessed by a pathologist. B, Representative images of chromogenic staining percentages are shown. C, Densities immune cell subsets were compared across PD-L1+ (>1%) and PD-L1- Capacity for production IFN-γ, IL-2, TNF-α D, CD8+ T cells E, CD4+ between Frequencies individual that compose all F, tumor-free G, Statistics generated Kruskal–Wallis test ranks (a,f,g) Wilcoxon rank sum tests...
<p>Multiplex immunofluorescence quantification of immune densities in metastatic tissues. A, Densities phenotype cells were compared between tumor-free and tumor-involved tissues across different organ sites. B, Pathologist evaluation percentage tissue area considered tumor-involved. C, Percentages ‘clean tumor’ within identified to be composed by stroma. total stroma as contributed 100um proximal (D) 200um (E) tumor alone. F, Representative composite images are shown for each type....
<div>Abstract<p>Immune composition within the tumor microenvironment (TME) plays a central role in propensity of cancer cells to metastasize and respond therapy. Previous studies have suggested that metastatic TME is immune-suppressed. However, limited accessibility multiple sites patients has made assessing immune difficult context multiorgan metastases. We utilized rapid postmortem tissue collection protocol assess numerous breast metastasis paired tumor-free tissues....
<p>Whole slide multiplex immunofluorescence representative images. Whole composite images (left) of staining for CD3 (green), CD8 (orange), FOXP3 (yellow), CD20 (cyan), CD68 (magenta), pan-CK (gray), DAPI (blue). Tissue segmentation (right) each whole image is shown cancer islands (red), stroma extending 100um bands (multiple colors) and adjacent tumor-free tissue. Representative slides are tumor-involved bone (A, B), brain (C, D), liver (E, F), lung (G, H), skin (I, J). Scale bars...