A5065092925- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- SARS-CoV-2 and COVID-19 Research
- Synthetic Organic Chemistry Methods
- CRISPR and Genetic Engineering
- Click Chemistry and Applications
- Fuel Cells and Related Materials
- Molecular Sensors and Ion Detection
- Pharmacological Receptor Mechanisms and Effects
- Computational Drug Discovery Methods
- Chemical Synthesis and Analysis
- Molecular Junctions and Nanostructures
- Anesthesia and Pain Management
- Nanoplatforms for cancer theranostics
- Photoreceptor and optogenetics research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant Pathogens and Fungal Diseases
- Botulinum Toxin and Related Neurological Disorders
- Graphene research and applications
- Pain Mechanisms and Treatments
- COVID-19 Clinical Research Studies
- Marine Sponges and Natural Products
- Asymmetric Hydrogenation and Catalysis
- Gene Regulatory Network Analysis
Nanjing University
2023-2025
State Key Laboratory of Analytical Chemistry for Life Science
2025
Nanjing Medical University
2020-2022
DNAzyme-based fluorescent probes for imaging metal ions in living cells have received much attention recently. However, employing situ within subcellular organelles, such as nucleus, remains a significant challenge. We developed three-stranded DNAzyme probe (TSDP) that contained 20-base-pair (20-bp) recognition site of CRISPR/Cas9, which blocks the activity. When Cas9, with its specialized nuclear localization function, forms an active complex sgRNA cell it cleaves TSDP at site, resulting...
Abstract DNAzyme‐based fluorescent probes for imaging metal ions in living cells have received much attention recently. However, employing situ within subcellular organelles, such as nucleus, remains a significant challenge. We developed three‐stranded DNAzyme probe (TSDP) that contained 20‐base‐pair (20‐bp) recognition site of CRISPR/Cas9, which blocks the activity. When Cas9, with its specialized nuclear localization function, forms an active complex sgRNA cell it cleaves TSDP at site,...
Targeted degradation of membrane proteins represents an attractive strategy for eliminating pathogenesis‐related proteins. Aptamer‐based chimeras hold great promise as protein degraders, however, their efficacy is often hindered by the limited structural stability and risk off‐target effects due to non‐covalent interaction with target We here report first design a covalent aptamer‐based autophagosome‐tethering chimera (CApTEC) enhanced autophagic cell‐surface proteins, including transferrin...
Targeted degradation of membrane proteins represents an attractive strategy for eliminating pathogenesis‐related proteins. Aptamer‐based chimeras hold great promise as protein degraders, however, their efficacy is often hindered by the limited structural stability and risk off‐target effects due to non‐covalent interaction with target We here report first design a covalent aptamer‐based autophagosome‐tethering chimera (CApTEC) enhanced autophagic cell‐surface proteins, including transferrin...
Abstract CRISPR‐LbuCas13a has emerged as a revolutionary tool for in vitro diagnosis. Similar to other Cas effectors, LbuCas13a requires Mg 2+ maintain its nuclease activity. However, the effect of divalent metal ions on trans‐cleavage activity remains less explored. Herein, we addressed this issue by combining experimental and molecular dynamics simulation analysis. In studies showed that both Mn Ca could replace cofactors LbuCas13a. contrast, Ni , Zn Cu or Fe inhibits cis‐ trans‐ cleavage...
A 3,4-dihydroisocoumarin derivative fused with dihydrothiophene, talarolactone (1), and two known compounds, terreusinone (2) 4,6-dihydroxy-5-methylphthalide (3), were isolated from Talaromyces sp. associated Xanthoparmelia angustiphylla. The structure of 1 was deduced extensive spectroscopic data, electronic circular dichroism calculations, X-ray diffraction analyses. plausible biosynthetic pathway further proposed. Compound showed selective antimigratory activity in a wound-healing assay...
A CRISPR–Cas13a-powered autocatalytic cleavage circuit (CRISPR-ACC) for ultrasensitive and specific detection of a circadian clock gene was developed.
Real-time dynamic imaging of immunoactivation-related cytokines is crucial for evaluating the efficacy immune checkpoint blockade therapy and optimizing treatment regimen. We introduce herein a spatiotemporally controlled nanodevice that allows in situ photoactivated interferon-gamma (IFN-γ) secretion from T cells vitro vivo. The constructed by rational engineering an aptamer-embedded, UV-cleavable PC-DNA probe further integration with upconversion nanoparticles- CRISPR-Cas12a-enhanced...
Global prevalence of coronavirus disease 2019 (COVID-19) calls for an urgent development anti-viral regime. Compared with the new drugs, drug repurposing can significantly reduce cost, time, and safety risks. Given fact that harnesses spike protein to invade host cells through angiotensin-converting enzyme 2 (ACE2), hence we see if any previous anti-virtual compounds block spike-ACE2 interaction inhibit virus entry. The results molecular docking dynamic simulations revealed remdesivir...
Current treatments for chronic neuropathic pain often fall short. A small-molecular compound ZL006 can suppress N-Methyl-d-aspartate receptor (NMDAR)-mediated behaviors without blocking essential NMDAR function and brings new hope therapy. The persistent nature of mandates the long-term treatment. However, similar to existing analgesics, has only a short duration action. To unleash therapeutic potential ZL006, stability in aqueous solutions is investigated, ZL006-incorporated P407-based...
The level of 25-hydroxyvitamin D3 [25(OH)VD3] in human blood is considered as the best indicator vitamin D status, and its deficiency or excess can lead to various health problems. Current methods for monitoring 25(OH)VD3 metabolism living cells have limitations terms sensitivity specificity are often expensive time-consuming. To address these issues, an innovative trident scaffold-assisted aptasensor (TSA) system has been developed online quantitative complex biological environments....