A5082450658- Analytical Chemistry and Sensors
- Advanced Chemical Sensor Technologies
- Gas Sensing Nanomaterials and Sensors
- Advanced Sensor and Energy Harvesting Materials
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Electrochemical Analysis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- RNA and protein synthesis mechanisms
- Tactile and Sensory Interactions
- Conducting polymers and applications
- Microbial infections and disease research
- Sensor Technology and Measurement Systems
- 2D Materials and Applications
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Olfactory and Sensory Function Studies
- Nanowire Synthesis and Applications
- Dysphagia Assessment and Management
- Biochemical and Structural Characterization
- Dielectric materials and actuators
- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
UES (United States)
2018-2024
United States Air Force Research Laboratory
2018-2024
Wright-Patterson Air Force Base
2018-2024
Integra (United States)
2024
711th Human Performance Wing
2018-2023
University of Cincinnati
2016-2019
University of Illinois Urbana-Champaign
2011-2016
Princeton Theological Seminary
2010
National Federation of the Blind
1990
University of California, Irvine
1977
A wearable sweat biosensing device is demonstrated that stimulates and continuously measures ethanol concentrations at 25 s intervals, which then correlated with blood during a >3 hour testing phase. The involves baseline condition (no ethanol) followed by rapid rise of (oral bolus), finally, the physiological response body as return to (metabolized). Data sets include multiple in vivo validation trials careful vitro characterization electrochemical enzymatic sensor against likely...
Sensors for human health and performance monitoring require biological recognition elements (BREs) at device interfaces the detection of key molecular biomarkers that are measurable state indicators. BREs, including peptides, antibodies, nucleic acids, bind to in vicinity sensor surface create a signal proportional biomarker concentration. The discovery BREs with required sensitivity selectivity low concentrations remains fundamental challenge. In this study, we describe an in-silico...
Real-time sensing of proteins, especially in wearable devices, remains a substantial challenge due to the need convert binding event into measurable signal that is compatible with chosen analytical instrumentation. Impedance spectroscopy enables real-time detection via either measuring electrostatic interactions or electron transfer reactions while simultaneously being amenable miniaturization for integration form-factors. To create more robust methodology optimizing impedance-based sensors,...
Electrowetting and electrocapillarity of liquid metals have a long history, recent explosion renewed interest. Liquid electromagnetic properties surface tensions (>500 mN/m) that enable new forms reconfigurable devices. However, the only nontoxic option, gallium alloys, suffer from immediate formation semirigid oxide. Although acids or electrochemical reduction can remove this oxide, these approaches surround alloy in fluid is also electrically conducting, diminishing effectiveness...
Breathing-air quality within commercial airline cabins has come under increased scrutiny because of the identification volatile organic compounds (VOCs) from engine bleed air used to provide oxygen cabins. Ideally, a sensor would be placed pipe itself, enabling detection before it permeated through and contaminated entire cabin. Current gas-phase sensors suffer issues with selectivity, do not have appropriate form factor, or are too complex for deployment. Here, we chose isopropyl alcohol...
Moving to ultra-low (<100 nL) sample volumes presents numerous challenges, many of which can be resolved by implementation open nanofluidic films. These films are fabricated using a hexagonal network gold-coated microchannels capture all the following innovative advantages: (1) <100 nL cm-2; (2) zero analyte exchange and loss with film materials; (3) rapid omni-directional wicking transport >500 min-1 per square film; (4) ultra-simple roll-to-roll fabrication; (5) stable bio-compatible...
The Respiration Collector for In Vitro Analysis (ReCIVA) sampler, marketed by Owlstone Medical, provides a step forward in exhaled breath sampling through active directly onto thermal desorption (TD) tubes. Although an improvement to the issues surrounding bag sampling, ReCIVA device, first released 2015, is relatively new research and clinical tool that requires further exploration. Here, data are presented comparing two distinct devices. results, serial numbers #33 #65, demonstrate overall...
In the past 5 years, real-time health monitoring has become ubiquitous with development of watches and rings that can measure report on physiological state. As an extension, biomarker sensors, such as continuous glucose monitor, are becoming popular for both performance monitoring. However, few sensors biomarkers have been made commercially available; this is primarily due to problems cost, stability, sensitivity, selectivity, reproducibility biosensors. Therefore, simple, robust needed...
Non-invasive wearable biosensors provide real-time, continuous, and actionable health information. However, difficulties detecting diluted biomarkers in excreted biofluids limit practical applications. Most of interest are transported paracellularly into from biomarker-rich blood interstitial fluid during normal modulation cellular tight junctions. Calcium chelators reversible junction modulators that have been shown to increase absorption across the intestinal epithelium. calcium not yet...
Abstract Chemical sensors based on solution‐processed 2D nanomaterials represent an extremely attractive approach toward scalable and low‐cost devices. Through the implementation of real‐time impedance spectroscopy development a three‐element circuit model, redox exfoliated MoS 2 nanoflakes demonstrate ultrasensitive empirical detection limit NO gas at 1 ppb, with extrapolated ultimate approaching 63 ppt. This sensor construct reveals more than three orders magnitude improvement from...
To take full advantage of the reagent- and label-free sensing capabilities electrochemical sensors, a frequent remaining challenge is interference degradation sensors due to uncontrolled pH or salinity in sample solution foulants from solution. Here, we present an oil-membrane sensor protection technique that allows for permeation hydrophobic (lipophilic) analytes into sealed compartment containing ideal conditions while simultaneously blocking common hydrophilic interferents (proteins,...
Due to several sources of potential variability associated with exhaled breath bag sampling procedures for off-line analysis, the Respiration Collector in vitro Analysis (ReCIVA) sampler was developed. Although designed improve upon pitfalls bags, ReCIVA remains a minimally studied research tool. In this manuscript, attributes are investigated among three individual tests, such as background contamination, control software version, performance different adsorbent tubes, duplicate sample...
Inhaled medications are commonplace for administering bronchodilators, anticholinergics, and corticosteroids. While they have a defined legitimate use, also used in sporting events as performance-enhancing drugs. These performance enhancers can be acquired via both legal (i.e., at pharmacy through over-the-counter or prescription) illicit black market foreign pharmacies) means, thus making monitoring procurement impossible. urine tests detect these pharmacological agents hours after been...
Pasteurella multocida toxin (PMT) is an AB that causes pleiotropic effects in targeted host cells. The N-terminus of PMT (PMT-N) considered to harbor the membrane receptor binding and translocation domains responsible for mediating cellular entry delivery C-terminal catalytic domain into cytosol. Previous studies have implicated gangliosides as receptors binding. To gain further insight interactions involved cell membranes, we explored role various components binding, utilizing four...
Multiplex electronic antigen sensors for detection of SARS-Cov-2 spike glycoproteins and hemagglutinin from influenza A are fabricated using scalable processes straightforward transition to economical mass-production. The utilize the sensitivity surface chemistry a 2D MoS2 transducer attachment antibody fragments in conformation favorable binding with no need additional linker molecules. To make devices, ultra-thin layers (3 nm) amorphous sputtered over pre-patterned metal electrical...
Insurmountable detection challenges will impede the development of many next-generation lab-on-a-chip devices (e.g., point-of-care and real-time health monitors). Here we present first membrane-based, microfluidic sample preconcentration method that is continuous, quantifiable, simple, capable working with any analyte. Forward osmosis rapidly concentrates analytes by removing water from a stream fluid. 10-100X possible in mere minutes. This requires careful selection semi-permeable membrane...
High throughput characterization and processing techniques are becoming increasingly necessary to navigate multivariable, data-driven design challenges for sensors electronic devices. For two-dimensional materials, device performance is highly dependent upon a vast array of material properties including the number layers, lattice strain, carrier concentration, defect density, grain structure. In this work, laser crystallization was used locally pattern transform hundreds regions amorphous...
Abstract Carbon nanotube (CNT) chemiresistors have emerged as miniaturized platforms for wearable volatile organic compound (VOC) sensors. As a promising biorecognition element (BRE), short peptide can functionalize CNT to be sensitive and selective target VOCs. However, unveiling the VOC‐optimized peptide‐CNT pair gas‐phase sensing remains unclear. Here, novel multimodal molecular toolset designing, building, probing suitable BRE‐CNT sensors using machine learning, dynamics, near‐edge X‐ray...
Impedance-based biosensors for microbes, such as yeast and bacteria, have been widely used an alternative to optical sensing techniques. Yet, impedance-based techniques require complex electronics data processing that are difficult implement. Ultrasensitive, drift-correcting electronic devices needed biological in environments. Organic electrochemical transistors (OECTs) provide high signal amplification at low voltages, but most studies focused extensively on semiconductor materials within...
Membrane localization domain (MLD) was first proposed for a 4-helix-bundle motif in the crystal structure of C1 Pasteurella multocida toxin (PMT). This is also found structures several clostridial glycosylating toxins (TcdA, TcdB, TcsL, and TcnA). The Ras/Rap1-specific endopeptidase (RRSP) module multifunctional autoprocessing repeats-in-toxins (MARTX) produced by Vibrio vulnificus has sequence homology to C1-C2 domains PMT, including putative MLD. We have determined solution MLDs PMT RRSP...
Abstract The demand for flexible biochemical sensors has increased with advances in computational functionality and wireless communication. Advances materials science biochemistry have enabled the development fabrication of biosensors selective detection biological analytes leveraging ink-printed technologies, including form-factors. However, despite these advances, minimal effort been devoted to translating multi-material, three-electrode electrochemical cell, which is widely regarded as...
Abstract The light chain of botulinum neurotoxin A (BoNT/A‐LC) is a Zn‐dependent protease that specifically cleaves SNAP25 the SNARE complex, thereby impairing vesicle fusion and neurotransmitter release at neuromuscular junctions. C‐terminus (residues 141–206) retains full activity for BoNT/A‐LC‐catalyzed cleavage P1‐P1' (Gln197‐Arg198). Using structure complex between BoNT/A‐LC as model to design SNAP25‐derived pseudosubstrate inhibitors (SNAPIs) prevent presentation scissile bond active...