A5053284377- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Thermoregulation and physiological responses
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Olfactory and Sensory Function Studies
- Ferrocene Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- Asthma and respiratory diseases
- Spectroscopy and Laser Applications
- Advanced Sensor and Energy Harvesting Materials
- Infection Control and Ventilation
- Mass Spectrometry Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Protein Kinase Regulation and GTPase Signaling
- Toxic Organic Pollutants Impact
- Insect Pheromone Research and Control
- Enzyme function and inhibition
- Terahertz technology and applications
- Metabolism and Genetic Disorders
- Air Quality Monitoring and Forecasting
- Inhalation and Respiratory Drug Delivery
- Computational Drug Discovery Methods
- Amino Acid Enzymes and Metabolism
- Ocular Surface and Contact Lens
711th Human Performance Wing
2017-2023
United States Air Force Research Laboratory
2021-2023
Wright-Patterson Air Force Base
2018-2023
Henry M. Jackson Foundation
2017-2020
Wright State University
2010
The Ohio State University
2006
University of Georgia
2005
Abstract This paper introduces super absorbent polymer valves and colorimetric sensing reagents as enabling components of soft, skin‐mounted microfluidic devices designed to capture, store, chemically analyze sweat released from eccrine glands. The valving technology enables robust means for guiding the flow an inlet location into a collection isolated reservoirs, in well‐defined sequence. Analysis these reservoirs involves color responsive indicator chloride concentration with formulation...
Sweat is a biofluid with several attractive attributes. However, investigation into sweat for biomarker discovery applications still in its infancy. To add support the use of as non-invasive media human performance monitoring, volunteer participants were subjected to physical exertion model using treadmill. Following exercise, was collected, aliquotted, and analyzed metabolite protein content via high-resolution mass spectrometry. Overall, proteomic analysis illustrates significant...
The Respiration Collector for In Vitro Analysis (ReCIVA) sampler, marketed by Owlstone Medical, provides a step forward in exhaled breath sampling through active directly onto thermal desorption (TD) tubes. Although an improvement to the issues surrounding bag sampling, ReCIVA device, first released 2015, is relatively new research and clinical tool that requires further exploration. Here, data are presented comparing two distinct devices. results, serial numbers #33 #65, demonstrate overall...
Single in-frame amber (UAG) codons are found in the genes encoding MtmB, MtbB, or MttB, methyltransferases initiating methane formation from monomethylamine, dimethylamine, trimethylamine, respectively, certain Archaea. The crystal structure of MtmB demonstrated that codon codes for pyrrolysine, 22nd genetically encoded amino acid nature. Previous attempts to visualize amber-encoded residue by mass spectrometry identified only lysine, leaving information on existence and pyrrolysine resting...
Isoprene is one of the most abundant and frequently evaluated volatile organic compounds in exhaled breath. Recently, several individuals with background levels isoprene have been identified. Here, case study data are provided for an individual, identified from a previous study, this low prevalence phenotype. It hypothesized that individual will illustrate at rest during exercise. At rest, subject (7.1 ppb) shows (μ= 14.2 ± 7.0 while control group illustrates significantly higher quantities...
Due to several sources of potential variability associated with exhaled breath bag sampling procedures for off-line analysis, the Respiration Collector in vitro Analysis (ReCIVA) sampler was developed. Although designed improve upon pitfalls bags, ReCIVA remains a minimally studied research tool. In this manuscript, attributes are investigated among three individual tests, such as background contamination, control software version, performance different adsorbent tubes, duplicate sample...
Inhaled medications are commonplace for administering bronchodilators, anticholinergics, and corticosteroids. While they have a defined legitimate use, also used in sporting events as performance-enhancing drugs. These performance enhancers can be acquired via both legal (i.e., at pharmacy through over-the-counter or prescription) illicit black market foreign pharmacies) means, thus making monitoring procurement impossible. urine tests detect these pharmacological agents hours after been...
Due to increased interest in the use of excreted sweat for biomarker discovery, data must be generated supporting sample collection and handling methods allow controlled, large-scale discovery studies performed. In this manuscript, twelve amino acids were quantitated from exercise-induced held at room temperature or a simulated body 37 °C up 90 min. The illustrate large dynamic range exists among sweat. Additionally, acid quantities vary across individuals same individual under different...
As the demand for real-time exercise performance feedback increases, excreted sweat has become a biosource of interest continuous human assessment. For to truly fulfill this requirement, analyte concentrations must be normalized adequately assess day-to-day differences within and among individuals. In manuscript, data are presented highlighting use accurate localized rate as means ion global metabolomic normalization. The results illustrate large variability individuals over course two...
The goal of this work was to design and implement a prototype software tool for the visualization analysis small molecule metabolite GC−MS LC−MS data biomarker discovery. key features Metabolite Differentiation Discovery Lab (MeDDL) platform include support manipulation large sets, tools provide multifaceted view individual experimental results, architecture amenable modification addition new algorithms components. MeDDL tool, through its emphasis on visualization, provides unique...
The HAPSITE-ER-TD (Hazardous Air Pollutants on Site Extended Range HAPSITE-ER) portable gas chromatograph–mass spectrometer (GC–MS) combines the sensitivity of a thermal desorption (TD) GC–MS with advantages field-portable instrumentation. Though previous iterations HAPSITE have been extensively evaluated in literature, performance assessment TD-equipped instrument is lacking. In this manuscript, variability response was established for both internal standards and test compounds across three...
Hypoxia-like incidents in-flight have increased over the past decade causing severe safety concerns across aviation community. As a result, need to monitor flight crews in real-time for onset of hypoxic conditions is paramount continued aeronautical safety. Here, events were simulated laboratory via reduced oxygen-breathing device determine effect recovery gas oxygen concentration (21% and 100%) on exhaled breath volatile organic compound composition. Data from samples collected both...
Sweat is emerging as a prominent biosource for real-time human performance monitoring applications. Although promising, sources of variability must be identified to truly utilize sweat biomarker In this proof-of-concept study, targeted metabolomics method was applied collected from the forearms participants in 12-week exercise program who ingested either low or high nutritional supplementation twice daily. The data establish use dried powder mass metabolomic normalization samples....
Ras proteins are membrane-bound GTPases that regulate essential cellular processes at the plasma membrane (PM). Constitutively active mutations of K-Ras, one three isoforms in mammalian cells, frequently found human cancers. Ferrocene derivatives, which elevate reactive oxygen species (ROS), have shown to block growth non-small cell lung cancers harboring oncogenic mutant K-Ras. Here, we tested a novel ferrocene derivative on pancreatic ductal adenocarcinoma and cancer. Our compound,...
Abstract Due to the overall low abundance of volatile compounds in exhaled breath, it is necessary preconcentrate sample prior traditional thermal desorption (TD) gas chromatography mass spectrometry analysis. While certain aspects TD tubes, such as storage, have been evaluated, many remain uncharacterized. Two common Tenax TA and Biomonitoring 5TD were evaluated for background content flow rate variability. The data illustrate that tubes highest number (23) contamination greater than 3x...
Exhaled breath research has been hindered by a lack of standardization in collection and analysis methodologies. Recently, the Respiration Collector for