A5083878884- Virus-based gene therapy research
- Virology and Viral Diseases
- Lipid metabolism and biosynthesis
- Fatty Acid Research and Health
- Bacteriophages and microbial interactions
- Muscle Physiology and Disorders
- Digital Holography and Microscopy
- Microbial Metabolic Engineering and Bioproduction
- Nutrition and Health in Aging
- Curcumin's Biomedical Applications
- Advanced X-ray Imaging Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Adipose Tissue and Metabolism
- Enzyme Catalysis and Immobilization
- Advanced Fluorescence Microscopy Techniques
- Diet and metabolism studies
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Infant Nutrition and Health
- Cell Image Analysis Techniques
- Natural Products and Biological Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neonatal Respiratory Health Research
- Metabolomics and Mass Spectrometry Studies
- Medicinal Plants and Neuroprotection
Abbott (United States)
2009-2023
Abbott Nutrition (United States)
2014-2023
Ross Laboratories (United States)
2000-2021
Discovery Laboratories (United States)
2020-2021
Institute of Post Graduate Medical Education and Research
2011
Cleveland Clinic
1992-1999
Leiden University
1998
MetroHealth Medical Center
1997
MetroHealth
1997
Cleveland Foundation
1996
The Saccharomyces cerevisiae protein ELO2p is involved in the elongation of saturated and monounsaturated fatty acids. Among several sequences with limited identity S. ELO2 gene, a consensus cDNA sequence was identified from LifeSeq(R) database Incyte Pharmaceuticals, Inc. Human liver amplified by PCR using oligonucleotides complementary to 5' 3' ends putative human sequence. resulting full-length sequence, termed HELO1, consisted 897 bp, which encoded 299 amino However, contrast expression...
The enzymes that are involved in the elongation of fatty acids differ terms substrates on which they act. To date, specifically biosynthesis polyunsaturated have not yet been identified. In an attempt to identify a gene(s) encoding enzyme(s) specific for gamma-linolenic acid (GLA) (18:3n-6), cDNA expression library was made from fungus Mortierella alpina. constructed yeast vector screened by measuring expressed elongase activity [conversion GLA dihomo-GLA (20:3n-6)] individual clone. this...
An RNA-dependent RNA polymerase is packaged within the virions of purified vesicular stomatitis virus, a nonsegmented negative-strand which carries out transcription genome into mRNAs both in vitro and vivo . The composed two virally encoded polypeptides: large protein L (240 kDa) phosphoprotein P (29 kDa). Recently, we obtained biologically active from insect cells following infection by recombinant baculovirus expressing gene. During purification Sf21 cells, addition to an fraction...
Muscle wasting or cachexia is caused by accelerated muscle protein breakdown via the ubiquitin–proteasome complex. We investigated effect of curcumin c3 complex (curcumin c3) on attenuation proteolysis using in vitro and vivo models. Our data indicate that as low 0·50 μg/ml was very effective significantly inhibiting (30 %; P < 0·05) tyrosine release from human skeletal cells, which reached a maximum level inhibition 60 % ( at 2·5 μg/ml. Curcumin also inhibited chymotrypsin-like 20S...
Abstract Necrotising enterocolitis (NEC) is a devastating disease that typically affects formula-fed premature infants, suggesting dietary components may influence pathogenesis. TAG are the major fat of infant formula, and their digestion requires pancreatic lipases, which be naturally deficient in neonates. We hypothesise NEC develops partly from accumulation incompletely digested long-chain TAG-containing unsaturated fatty acids within intestinal epithelial cells, leading to oxidative...
Abstract Background The cellular mechanisms underlying the age‐associated loss of muscle mass and function (sarcopenia) are poorly understood, hampering development effective treatment strategies. Here, we performed a detailed characterization age‐related pathophysiological changes in mouse neuromuscular system. Methods Young, adult, middle‐aged, old (1, 4, 14, 24–30 months old, respectively) C57BL/6J mice were used. Motor behavioural electrophysiological tests histological...
The Saccharomyces cerevisiae protein ELO2p is involved in the elongation of saturated and monounsaturated fatty acids. Among several sequences with limited identity S. cerevisiaeELO2 gene, a consensus cDNA sequence was identified from LifeSeq® database Incyte Pharmaceuticals, Inc. Human liver amplified by PCR using oligonucleotides complementary to 5´ 3´ ends putative human sequence. resulting full-length sequence, termed HELO1, consisted 897bp, which encoded 299 amino However, contrast...
The phosphorylation of the P protein vesicular stomatitis virus by cellular casein kinase II (CKII) is essential for its activity in viral transcription. Recent vitro studies have demonstrated that CKII converts inactive unphosphorylated form (P0) to an active phosphorylated P1, after at two serine residues, Ser-59 and Ser-61. To gain insight into role CKII-mediated structure function protein, we carried out circular dichroism (CD) biochemical analyses both P0 P1. results CD reveal P1...
ABSTRACT The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit the viral RNA-dependent RNA polymerase complex. protein phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within amino-terminal acidic domain I P must be for vivo transcription activity, but not replication To examine role phosphorylation carboxy-terminal II replication, we have used panel mutant proteins which phosphate acceptor...
The phosphoprotein (P) of vesicular stomatitis virus (VSV) serotypes New Jersey [P(NJ)] and Indiana [P(I)] contains a highly conserved carboxy-terminal domain which is required for binding to the cognate N-RNA template as well form soluble complex with nucleocapsid protein Nin vivo.We have shown that deletion 11 amino acids from C-terminal end P(I) abolishes both forming activity N protein. Within this region, there are basic acid residues (R260 K262) potential candidates such interactions....
Abstract Paracellular permeability (PCP) is governed by tight junctions (TJs) in epithelial cells, acting as cell–cell adhesion structures, the aberration of which known to be linked dissociation and metastasis breast cancer cells. This study hypothesized that function TJs human cells can augmented gamma linolenic acid (GLA), selenium (Se), iodine (I) presence 17‐β‐estradiol, these molecules are increase TJ functions endothelial using assays trans‐epithelial resistance (TER), PCP,...
Abstract Achieving military mission objectives requires high levels of performance from Airmen who operate under extreme physical and cognitive demands. Thus, there is a critical need to establish scientific interventions enhance fitness performance—promoting the resilience aiding in success. We therefore conducted comprehensive, 12-week randomized controlled trial active-duty Air Force ( n = 148) compare efficacy multimodal intervention comprised high-intensity interval aerobic strength...
Due to increased interest in the use of excreted sweat for biomarker discovery, data must be generated supporting sample collection and handling methods allow controlled, large-scale discovery studies performed. In this manuscript, twelve amino acids were quantitated from exercise-induced held at room temperature or a simulated body 37 °C up 90 min. The illustrate large dynamic range exists among sweat. Additionally, acid quantities vary across individuals same individual under different...
Protein kinase activities associated with a highly purified transcriptionally active ribonucleoprotein complex from the virions of vesicular stomatitis virus (VSV) were isolated and characterized. Based upon several biochemical immunological criteria, protein activity, which phosphorylated bacterially expressed unphosphorylated (Po) protein, was shown to be cellular casein II (CKII). These studies included inhibition by specific inhibitors, phosphorylation mutant phosphoproteins (P),...
Abstract Tight junctions (TJs) in endothelial cells act as cell–cell adhesion structures, governing paracellular permeability (PCP). Disruption can lead to leaky vascular bed and potentially oedema swelling of tissues, the aetiology mastalgia. These changes may also cause spread cancer cells. This study aimed determine whether function TJs be strengthened by gamma linolenic acid (GLA), selenium (Se) iodine (I) presence 17beta‐estradiol (17β‐estradiol), which causes leakage disruption...
Deficient myelination of the brain is associated with neurodevelopmental delays, particularly in high-risk infants, such as those born small relation to their gestational age (SGA). New methods are needed further study this condition. Here, we employ Color Spatial Light Interference Microscopy (cSLIM), which uses a brightfield objective and RGB camera generate pathlength-maps nanoscale sensitivity conjunction regular image. Using tissue sections stained Luxol Fast Blue, myelin structures...
The Cognitive Ageing, Nutrition and Neurogenesis trial hypothesizes that a combined intervention with long-chain n-3 polyunsaturated fatty acids (n-3) cocoa flavan-3-ols (FLAV) will mitigate the cognitive decline anticipated to naturally occur over 1 year in older adults.In double-blinded, placebo-controlled parallel design, 259 individuals mild impairment or subjective memory were randomized control FLAV group (1.5 g docosahexaenoic acid + eicosapentaenoic 500 mg daily) for 12 months....
Abstract Necrotising enterocolitis (NEC) is a devastating gastrointestinal disease of prematurity that typically develops after the administration infant formula, suggesting link between nutritional components and development. One most significant complications in patients with NEC severe lung injury. We have previously shown formula enriched pre-digested Triacylglyceride do not require lipase action can significantly reduce severity mouse model. now hypothesise this ‘pre-digested fat (PDF)...