A5082677888- Virus-based gene therapy research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- interferon and immune responses
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Fish Biology and Ecology Studies
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
- COVID-19 Impact on Reproduction
- Insect symbiosis and bacterial influences
- RNA Research and Splicing
- Fish biology, ecology, and behavior
- Viral Infectious Diseases and Gene Expression in Insects
- vaccines and immunoinformatics approaches
- Inflammasome and immune disorders
- Viral Infections and Outbreaks Research
- Peptidase Inhibition and Analysis
National Institute of Pharmaceutical Education and Research
2025
University of Nebraska–Lincoln
2014-2024
Chilika Development Authority
2006-2019
University of Miami
1995-2000
University of Illinois Urbana-Champaign
1997
University of Alabama
1992
University of Alabama at Birmingham
1990-1991
University of California, Los Angeles
1986
Griffith University
1984
The double-stranded (ds) RNA-dependent protein kinase PKR is considered to play an important role in interferon's (IFN's) response viral infection. Here, we demonstrate that mice lacking are predisposed lethal intranasal infection by the usually innocuous vesicular stomatitis virus, and also display increased susceptibility influenza virus Our data indicate normal cells, primarily prevents replication inhibiting translation of mRNAs through phosphorylation eIF2α, while concomitantly...
ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 5 (GP5) is the most abundant envelope a major inducer of neutralizing antibodies in vivo. Three putative N-linked glycosylation sites (N34, N44, N51) are located on GP5 ectodomain, where neutralization epitope also exists. To determine which these used for role glycan moieties antibody response, we generated panel mutants containing amino acid substitutions at sites. Biochemical studies with expressed wild-type...
ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine leads to a serious disease characterized by delayed defective adaptive immune response. It is hypothesized that suboptimal innate response responsible for the pathogenesis. In study presented here we tested this hypothesis identified several nonstructural proteins (NSPs) with evasion properties encoded PRRS viral genome. Four total ten PRRSV NSPs were found have strong moderate inhibitory effects on beta...
ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) contains the major glycoprotein, GP5, as well three other minor glycoproteins, namely, GP2a, GP3, GP4, on virion envelope, all of which are required for generation infectious virions. To study their interactions with each cellular receptor PRRSV, we have cloned viral glycoproteins CD163 in expression vectors examined interaction transfected cells by coimmunoprecipitation (co-IP) assay using monospecific antibodies. Our...
Passive administration of porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing antibodies (NAbs) can effectively protect pigs against PRRSV infection. However, after infection, typically develop a weak deferred NAb response. One major reason for such meager response is the phenomenon glycan shielding involving GP5, glycoprotein carrying one epitope. We describe here type II field isolate (PRRSV-01) that highly susceptible to neutralization induces an atypically rapid,...
Zika virus (ZIKV), a mosquito-transmitted flavivirus responsible for sporadic outbreaks of mild and febrile illness in Africa Asia, reemerged the last decade causing serious human diseases, including microcephaly, congenital malformations, Guillain-Barré syndrome. Although genomic phylogenetic analyses suggest that genetic evolution may have led to enhanced virulence ZIKV, experimental evidence supporting role specific changes is currently lacking. One sequence motif, VNDT, containing an...
Replication and amplification of RNA genomes defective interfering (DI) particles vesicular stomatitis virus (VSV) depend on the expression viral proteins have until now been attained only in cells coinfected with helper VSV. In work described this report, we used a recombinant vaccinia virus-T7 polymerase system to synthesize individual VSV transfected plasmid DNAs that contain cDNA copies genes downstream T7 promoter. way, were able examine ability proteins, individually combination,...
ABSTRACT The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit the viral RNA polymerase. In previous studies, we demonstrated that insertion 19 amino acids in hinge region protein had no significant effect on P function. present study, inserted full-length enhanced green fluorescent (eGFP) frame into and show fusion (PeGFP) functional genome transcription replication, albeit with reduced activity. A recombinant encoding PeGFP place (VSV-PeGFP), which possessed growth...
In a genome-wide small interfering RNA (siRNA) screen, we recently identified the interferon (IFN)-inducible protein 35 (IFI35; also known as IFP35) factor required for vesicular stomatitis virus (VSV) infection. Studies reported here were conducted to further understand role and requirement of IFI35 in VSV Consistent with siRNA screening data, found that depletion led reduced replication at level viral gene expression. Although no direct interaction machinery was observed, negatively...
Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for infection. Using vesicular stomatitis virus (VSV), a prototypic NS family Rhabdoviridae , we conducted human genome-wide siRNA screen identified 72 genes viral Many of these were also infection by two other viruses, lymphocytic choriomeningitis Arenaviridae parainfluenza type 3 Paramyxoviridae...
Zika virus (ZIKV), a mosquito-transmitted Flavivirus, emerged in the last decade causing serious diseases and affecting human health globally. Currently, no licensed vaccines or antivirals are available to combat ZIKV, although several vaccine candidates pipeline. In recent years, presence of non-canonical G-quadruplex (GQ) secondary structures viral genomes has ignited significant attention as potential targets for antiviral strategy. this study, we identified novel conserved GQ by...
The severe consequences of the Zika virus (ZIKV) infections resulting in congenital syndrome infants and autoimmune Guillain–Barre adults warrant development safe efficacious vaccines therapeutics. Currently, there are no approved treatment options for ZIKV infection. Herein, we describe a bacterial ferritin-based nanoparticle vaccine candidate ZIKV. viral envelope (E) protein domain III (DIII) was fused in-frame at amino-terminus ferritin. displaying DIII examined its ability to induce...
Phosphorylation by casein kinase II at three specific residues (S-60, T-62, and S-64) within the acidic domain I of P protein Indiana serotype vesicular stomatitis virus has been shown to be critical for in vitro transcription activity viral RNA polymerase (P-L) complex. To examine role phosphorylation as well replication vivo, we used a panel mutant proteins which phosphate acceptor sites were substituted with alanines or other amino acids. Analyses alanine-substituted ability support...
ABSTRACT Previous studies from our laboratory revealed that cellular poly(C) binding protein 2 (PCBP2) downregulates vesicular stomatitis virus (VSV) gene expression. We show here VSV infection induces the formation of granular structures in cytoplasm containing RNA-binding proteins, including PCBP2, T-cell-restricted intracellular antigen 1 (TIA1), and TIA1-related (TIAR). Depletion TIA1 via small interfering RNAs (siRNAs), but not depletion TIAR, results enhanced growth The VSV-induced...