A5065789182- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- RNA and protein synthesis mechanisms
- Diabetes and associated disorders
- Virus-based gene therapy research
- Respiratory viral infections research
- Cardiomyopathy and Myosin Studies
- Animal Disease Management and Epidemiology
- Legionella and Acanthamoeba research
- Herpesvirus Infections and Treatments
- RNA regulation and disease
- Celiac Disease Research and Management
- Animal Virus Infections Studies
- Pancreatic function and diabetes
- Cardiovascular Effects of Exercise
- Immune Cell Function and Interaction
- Antibiotic Resistance in Bacteria
- Immunodeficiency and Autoimmune Disorders
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Pancreatitis Pathology and Treatment
- interferon and immune responses
- Eosinophilic Disorders and Syndromes
- Influenza Virus Research Studies
University of Nebraska Medical Center
2015-2024
Institut Pasteur
2018
Veterinary and Agrochemical Research Centre
2018
Nebraska Medical Center
2003-2017
University of Nebraska at Omaha
2004-2012
Creighton University
2006
Kyoto University
1992-2004
Covance (United States)
2004
Omaha VA Medical Center
2003
Epirus Biopharmaceuticals (United States)
2000
Analysis of enteroviral genomes has revealed that the 5' nontranslated region is highly conserved, providing consensus sequences for design oligonucleotides which should anneal to most, if not all, human RNAs. We designed and used a pair such generic primers enzymatically amplify cDNA from coxsackievirus group B types 1 through 6, poliovirus 3, 4 A types, 29 echoviruses. The polymerase chain reaction (PCR) products generated with these were analyzed by agarose gel electrophoresis, Southern...
ABSTRACT Insulin-dependent (type 1) diabetes mellitus (T1D) onset is mediated by individual human genetics as well undefined environmental influences such viral infections. The group B coxsackieviruses (CVB) are commonly named putative T1D-inducing agents. We studied CVB replication in nonobese diabetic (NOD) mice to assess how infection diverse strains affected T1D incidence a model of T1D. Inoculation 4- or 8-week-old NOD with any nine different significantly reduced the 2- 10-fold over...
ABSTRACT Adult human enteroviral heart disease is often associated with the detection of RNA in cardiac muscle tissue absence infectious virus. Passage coxsackievirus B3 (CVB3) adult murine cardiomyocytes produced CVB3 that was noncytolytic HeLa cells. Detectable but noncytopathic also isolated from hearts mice inoculated CVB3. Sequence analysis revealed five classes genomes 5′ termini containing 7, 12, 17, 30, and 49 nucleotide deletions. Structural changes (assayed by chemical...
We report the construction of chimeric coxsackievirus B3 (CVB3) strains in which sequences an infectious cDNA copy a noncardiovirulent CVB3 genome were replaced by homologous from cardiovirulent to identify 10 predicted genetic sites determine cardiovirulence. Cardiovirulent phenotype expression was consistently linked nucleotide 234 (U and C avirulent CVB3) 5' nontranslated region. Reconstructions parental chimeras restored when tested mice. Inoculation severe combined immunodeficient...
Enterovirus infections are generally acute and rapidly cleared by the host immune response. Enteroviruses can at times persist in immunologically intact individuals after rise of type‐specific neutralizing The mechanism enterovirus persistence was shown group B coxsackieviruses (CVB) to be due naturally‐occurring deletions 5′ terminus genome which variably impact stem‐loop secondary structure called domain I. These result much slower viral replication a loss measurable cytopathic effect when...
Coxsackievirus B3 (CVB3) infections can cause myocarditis in humans and are implicated the pathogenesis of dilated cardiomyopathy. The natural genetic determinants cardiovirulence for CVB3 have not been identified, although using strains engineered laboratory, identified 5′ nontranslated region (5′NTR) capsid. myocarditic phenotypes two clinical isolates were determined an established murine model inflammatory heart disease. 5′NTRs capsid proteins noncardiovirulent CVB3/CO strain...
Although echovirus 22 (EV22) is classified as an enterovirus in the family Picornaviridae, it atypical of paradigm, typified by polioviruses and coxsackie B viruses. cDNA reverse transcribed from coxsackievirus B3 (CVB3) RNA does not hybridize to genomic EV22, conversely, made EV22 CVB3 or molecular clones poliovirus type 1. viral encephalomyocarditis virus a clone Theiler's murine encephalomyelitis virus, members cardiovirus genus. The cannot be detected polymerase chain reaction using...
Background: Group B enteroviruses are common causes of acute myocarditis, which can be a precursor chronic myocarditis and dilated cardiomyopathy, leading heart transplantation. To date, the specific viral functions involved in development cardiomyopathy remain unclear. Methods: Total RNA from cardiac tissue patients with was extracted, sequences corresponding to 5’ termini enterovirus RNAs were identified. After next-generation sequencing, cDNA clones mimicking found patient tissues...
BACKGROUND In our model of dilated cardiomyopathy (DCM), cardiac dilatation and hypertrophy developed after inoculation encephalomyocarditis virus (EMCV), but the infectious was isolated only early infection. this study, we investigated whether viral RNA could be detected at later times using polymerase chain reaction (PCR). METHODS AND RESULTS in vitro FL (human amnion) cells infected with EMCV were harvested for extraction, cDNA synthesized by reverse transcription random hexamers. Using...
ABSTRACT Primary cultures of human umbilical vein endothelial cells (HUVEC) express the coxsackievirus and adenovirus receptor (HCAR). Whereas HCAR expression in HeLa was constant with respect to cell density, HUVEC increased culture confluence. not quantitatively altered by infection B.