A5069866852- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CAR-T cell therapy research
- interferon and immune responses
- Ocular Diseases and Behçet’s Syndrome
- Viral gastroenteritis research and epidemiology
- Acute Myeloid Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Heparin-Induced Thrombocytopenia and Thrombosis
- Hematopoietic Stem Cell Transplantation
- COVID-19 Clinical Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Long-Term Effects of COVID-19
- Galectins and Cancer Biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related gene regulation
- Animal Virus Infections Studies
- Immune responses and vaccinations
- Multiple Sclerosis Research Studies
- SARS-CoV-2 and COVID-19 Research
- IL-33, ST2, and ILC Pathways
National Heart Lung and Blood Institute
2019-2024
National Institutes of Health
2014-2024
Lentigen Technology (United States)
2024
University of Nebraska–Lincoln
2011-2022
National Institute of Allergy and Infectious Diseases
2014-2020
Indian Veterinary Research Institute
2009-2014
Regulatory T cells (Tregs) play a cardinal role in the immune system by suppressing detrimental autoimmune responses, but their acute, chronic infectious diseases and tumor microenvironment remains unclear. We recently demonstrated that IFN-α/β receptor (IFNAR) signaling promotes Treg function autoimmunity. Here we dissected functional of IFNAR-signaling Tregs using Treg-specific IFNAR deficient (IFNARfl/flxFoxp3YFP-Cre) mice acute LCMV Armstrong, Clone-13 viral infection, models. In both...
Host conditioning has emerged as an important component of effective adoptive cell transfer–based immunotherapy for cancer. High levels IL-1β are induced by host conditioning, but its impact on the antitumor function T cells remains unclear. We found that administration increased population size and functionality adoptively transferred within tumor. Most importantly, enhanced ability tumor-specific to trigger regression large, established B16 melanoma tumors in mice. Mechanistically, we...
CD47 is a ubiquitous cell surface receptor that directly regulates T immunity by interacting with its inhibitory ligand thrombospondin-1 and limits clearance of cells phagocytes express counter-receptor signal-regulatory protein-α. Murine natural killer (NK) higher levels than other lymphocytes, but the role in regulating NK homeostasis immune function remains unclear. Cd47-/- mice exhibited depletion precursors bone marrow, consistent antiphagocytic CD47. In contrast, antisense knockdown or...
Urine is a metabolite-rich biofluid that reflects the body's effort to maintain chemical and osmotic homeostasis. Clinical diagnosis routinely relies on urine samples because collection process easy noninvasive. Despite these advantages, an under-investigated source of biomarkers for multiple sclerosis (MS). Nuclear magnetic resonance spectroscopy (NMR) has become common approach analyzing urinary metabolites disease biomarker discovery. For illustration potential diagnosing treating MS...
Foxp3 is regarded as the major transcription factor for T regulatory (T reg ) cells and expression of used to identify quantitate Treg in mouse models. However, several studies have demonstrated that human CD4 + conventional conv activated vitro by cell receptor (TCR) stimulation can express Foxp3. This observation has raised doubt suitability a marker man. Helios, member Ikaros gene family, been shown be expressed 80-90% potentially serve . Here, we confirm readily upregulated upon TCR ,...
Abstract Background Tetramers are useful tools to enumerate the frequencies of antigen-specific T cells. However, unlike CD8 cells, CD4 cells - especially self-reactive challenging detect with major histocompatibility complex (MHC) class II tetramers because low and affinities their cell receptors MHC-peptide complexes. Here, we report use fluorescent multimers, designated MHC dextramers that contain a large number peptide-MHC complexes per reagent. Results The utility was evaluated in three...
Despite multi-model therapy of maximal surgical resection, radiation, chemotherapy, and tumor-treating fields, the median survival glioblastoma (GBM) patients is less than 15 months. Protein arginine methyltransferase 5 (PRMT5) catalyzes symmetric dimethylation residues overexpressed in GBM. Inhibition PRMT5 causes senescence stem-like GBM tumor cells. LB100, a first-in-class small molecular inhibitor protein phosphatase 2A (PP2A), can sensitize therapy-resistant Here, we tested anti-GBM...
Abstract Coxsackievirus B3 (CVB3), is commonly implicated in myocarditis, which can lead to dilated cardiomyopathy, addition causing acute pancreatitis and meningitis. Yet, no vaccines are currently available prevent this infection. Here, we describe the derivation of a live attenuated vaccine virus, termed mutant (Mt) 10, encoding single amino acid substitution H790A within viral protein 1, that prevents CVB3 infection mice protects from both myocarditis challenge studies. We noted animals...
Coxsackievirus group B (CVB) contains six serotypes that can affect various organs. Some of these organ-specific diseases such as myocarditis and pancreatitis be caused by more than one serotype. Thus, development immunological tools common to multiple is desired. This especially critical for analyzing antigen-specific T cell responses at a single level. To this end, we made efforts identify the immunogenic epitopes CVB3 leading us localize three within viral protein 1 (VP1) namely, VP1...
We report here the utility of major histocompatibility complex (MHC) class II dextramers for in situ detection self-reactive CD4 T cells two target organs, brain and heart. optimized conditions antigen-specific using sections obtained from SJL mice immunized with myelin proteolipid protein (PLP) 139–151; were costained IAs/PLP 139–151 (specific) or Theiler's murine encephalomyelitis virus (TMEV) 70–86 (control) anti-CD4. Analysis by laser scanning confocal microscope revealed positive PLP...
We recently reported that Acanthamoeba castellanii (ACA), an opportunistic pathogen of the central nervous system (CNS) possesses mimicry epitopes for proteolipid protein (PLP) 139–151 and myelin basic 89–101, induce experimental autoimmune encephalomyelitis (EAE) in SJL mice reminiscent diseases induced with their corresponding cognate peptides. now demonstrate infected ACA also show generation cross-reactive T cells, predominantly PLP 139–151, as evaluated by cell proliferation...
Sarcoplasmic/endoplasmic reticulum Ca2+ adenosine triphosphatase (SERCA)2a, a critical regulator of calcium homeostasis, is known to be decreased in heart failure. Patients with myocarditis or dilated cardiomyopathy develop autoantibodies SERCA2a suggesting that they may have pathogenetic significance. In this report, we describe epitope mapping analysis A/J mice leads us make five observations: 1) contains multiple T cell epitopes induce varying degrees myocarditis. One epitope, 971-990,...
Abstract Allogeneic hematopoietic cell transplantation (allo-HCT) offers a curative option for patients with certain non-malignant hematological diseases. High-dose post-transplant cyclophosphamide (PT-Cy) (200 mg/kg) and sirolimus (3 mg/kg), (HiC) synergistically induce stable mixed chimerism. Further, cytotoxic T lymphocyte-associated antigen-4 immunoglobulin (CTLA4-Ig), also known as Abatacept (Aba), promote immune tolerance allograft survival. Here, in major histocompatibility complex...