A5065078301- Viral Infections and Immunology Research
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Cardiomyopathy and Myosin Studies
- Cytomegalovirus and herpesvirus research
- Animal Disease Management and Epidemiology
- Parvovirus B19 Infection Studies
- Microbial infections and disease research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Cardiovascular Effects of Exercise
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Vaccine Coverage and Hesitancy
- Antimicrobial Peptides and Activities
- Pneumonia and Respiratory Infections
- Viral Infectious Diseases and Gene Expression in Insects
- Tracheal and airway disorders
- RNA Research and Splicing
- Congenital Anomalies and Fetal Surgery
- Animal Behavior and Welfare Studies
- Congenital heart defects research
- Polyomavirus and related diseases
- HIV Research and Treatment
Université de Montréal
2025
Oniris
2017-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2024
Laboratoire de Génie des Procédés – Environnement – Agro-alimentaire
2018-2024
Biologie Epidémiologie et Analyse de Risque en santé animale
2016-2024
Université de Reims Champagne-Ardenne
2008-2019
Université Nantes Angers Le Mans
2017-2019
Laboratoire D'étude des Résidus et Contaminants Dans les Aliments
2017-2019
Université Bretagne Loire
2018
Max Planck Institute for Informatics
2018
From January to December 2007, 973 stool specimens were prospectively collected from children hospitalized for gastroenteritis signs or neonates and premature cases who born in two French hospital settings the north of France. They tested by rapid enzyme immunoassay (EIA) analyses rotavirus adenovirus commercially available ELISA tests detection norovirus astrovirus. The overall rates prevalence rotavirus, norovirus, adenovirus, astrovirus 21, 13, 5, 1.8%, respectively, they did not...
We prospectively tested 95 nasal swabs or nasopharyngeal aspirates taken from 56 adults and 39 children visiting the Reims University Medical Centre (northern France) for influenza-like illnesses (ILI) during early stage of French influenza A/H1N1v pandemic (October 2009). Respiratory samples were using a combination two commercially available reverse transcription-PCR (RT-PCR) DNA microarray systems allowing rapid detection A virus strains, including new strain as well 20 other common newly...
Abstract Newly available molecular tools allow a sensitive detection of broad panel viruses in respiratory tract specimens. In the present study, application multiplex RT‐PCR DNA microarray diagnosis and epidemiological survey viral infections infants hospitalized for bronchiolitis was assessed. One hundred thirty‐eight nasopharyngeal aspirates collected from October 2007 to September 2008 were tested by direct immunofluorescence culture, combination referenced RT‐PCRs microarray. or more...
Acute Respiratory Infections (ARIs) are responsible for considerable morbidity and mortality worldwide. Documentation of respiratory specimens can help an appropriate clinical management with a significant effect on the disease progress in patient, antimicrobial therapy used risk secondary spread infection. Here, we compared performances four commercial multiplex kits French University Hospital diagnostic microbiology laboratories detection ARI pathogens (i.e., xTAG Viral Panel Fast,...
Few studies have evaluated the contribution of multiple virus and bacterial infections in acute exacerbation chronic obstructive pulmonary disease. This study estimated burden viral respiratory moderate to very severe disease patients that were prospectively followed-up during a 12-month pilot study. Clinical data collected monthly sputum was at time each event. Classical culture techniques for bacteria multiplex polymerase chain reaction (PCR) microarray detection assays performed identify...
Background: Group B enteroviruses are common causes of acute myocarditis, which can be a precursor chronic myocarditis and dilated cardiomyopathy, leading heart transplantation. To date, the specific viral functions involved in development cardiomyopathy remain unclear. Methods: Total RNA from cardiac tissue patients with was extracted, sequences corresponding to 5’ termini enterovirus RNAs were identified. After next-generation sequencing, cDNA clones mimicking found patient tissues...
Recoding strategies have emerged as a promising approach for developing safer and more effective vaccines by altering the genetic structure of microorganisms, such viruses, without changing their proteins. This method enhances vaccine safety efficacy while minimizing risk reversion to virulence. frequency CpG dinucleotides, which in turn activates immune responses ensures strong attenuation pathogens. Recent advancements highlight synonymous recoding's potential, offering improved stability...
Although numerous reports have described inflammatory bowel diseases (IBDs) complicated with cytomegalovirus (CMV) infection, the virus participation as an exacerbating factor remains unclear. The aim of this study was thus to clarify clinical significance CMV infection complicating exacerbation and correlate detection various characteristics in IBD patients. Sixty-seven colonic biopsies obtained from 53 patients admitted for were retrospectively analyzed by real-time PCR assay. genome...
Viruses are the main etiological cause of central nervous system (CNS) infections. A rapid molecular diagnosis is recommended to improve therapeutic management patients. The aim this study was evaluate performances a DNA microarray, Clart Entherpex kit (Genomica, Coslada, Spain), allowing and simultaneous detection 9 RNA neurotropic viruses: herpes simplex virus 1 (HSV-1), HSV-2, varicella-zoster (VZV), cytomegalovirus (CMV), Epstein-Barr (EBV), human herpesvirus 6 (HHV-6), HHV-7, HHV-8,...
Enterovirus 68 was detected in 10 respiratory specimens from pediatric patients hospitalized for acute wheezing or bronchitis during 2009 the northeast of France. Viral loads ranged 2 × 10(5) to 7.2 10(7) copies/ml. Alignment 5' nontranslated regions and phylogenetic analysis partial VP1 gene sequences show that these viruses clustered belonged clade C.
Abstract We performed deep sequencing analysis of the enterovirus 5′ noncoding region in cardiac biopsies from a patient with dilated cardiomyopathy. Results displayed mix deleted and full-length coxsackievirus B3, characterized by low viral RNA load (8.102 copies/μg nucleic acids) positive-sense to negative-sense ratio 4.8.
Coinfections affecting the porcine respiratory system have often been overlooked, in favor of mono-infections, even though they are significantly more common field. In pigs, term 'porcine complex' is used to describe coinfections involving both viruses, such as, for example, swine influenza type A virus (swIAV), and reproductive syndrome (PRRSV), circovirus 2 (PCV-2), as well bacteria. Until recently, most studies were primarily focused on clinical aspects paid little attention molecular...
We examined the impact of enterovirus (EV) cardiac replication activity on endomyocardial mitochondrial pathway in patients with acute myocarditis.Levels apoptotic cardiomyocytes were determined by TUNEL and ligation-mediated polymerase chain reaction (PCR) assays EV was assessed immunostaining VP1 capsid protein ventricular myocytes myocarditis (n = 25), healthy heart controls 15). Ratio cytosolic/mitochondrial cytochrome c concentrations ELISA assay, levels active caspase-9 western blot...
ABSTRACT Herpes simplex virus 1 (HSV-1) esophagitis diagnosis is routinely based on the endoscopic findings confirmed by histopathological examination of lesions. Virological not systematically performed and restricted to viral culture or qualitative PCR assay from biopsy specimens. The aim this study was assess interest quantitative real-time in HSV-1 comparing results obtained those histological associated with immunohistochemical staining, which considered “gold standard.” From 53...
Standardized one-step real-time RT-PCR assay detected enterovirus RNA in cardiac biopsy samples from 4 of 20 patients suffering idiopathic dilated cardiomyopathy (IDCM). The median viral load was 287 copies per microgram total extracted nucleic acids, with positive- to negative-strand ratios ranging 2 20. These results demonstrate persistence the heart IDCM patients, characterized by low loads and negative-RNA ratios.
Viruses are the leading cause of central nervous system (CNS) infections, ahead bacteria, parasites, and fungal agents. A rapid comprehensive virologic diagnostic testing method is needed to improve therapeutic management hospitalized pediatric or adult patients. In this study, we assessed clinical performance PCR amplification coupled with electrospray ionization-time flight mass spectrometry analysis (PCR-MS) for diagnosis viral CNS infections. Three hundred twenty-seven cerebrospinal...
Viral detection in heart tissues has become a central issue for the diagnosis and exploration of pathogenesis idiopathic dilated cardiomyopathy (IDCM). In present study, common cardiotropic viruses 67 explanted samples 31 IDCM adult patients were detected semiquantified by using first time new technology based on PCR assay coupled to electrospray ionization-time flight mass spectrometry analysis (PCR-MS), with comparison reference quantitative real-time (RT-qPCR) assay. PCR-MS identified...