A5020545173- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Medical Imaging and Pathology Studies
- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Inhalation and Respiratory Drug Delivery
- Extracellular vesicles in disease
- Occupational and environmental lung diseases
- Hedgehog Signaling Pathway Studies
- Sperm and Testicular Function
- Systemic Sclerosis and Related Diseases
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Pulmonary Hypertension Research and Treatments
- Occupational exposure and asthma
- Renal and related cancers
- Inflammasome and immune disorders
- Neurogenetic and Muscular Disorders Research
- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Histiocytic Disorders and Treatments
- Testicular diseases and treatments
- Reproductive Biology and Fertility
- Transplantation: Methods and Outcomes
- RNA regulation and disease
- Sarcoidosis and Beryllium Toxicity Research
Nanjing University
2017-2025
Nanjing University of Chinese Medicine
2022-2025
Nanjing General Hospital of Nanjing Military Command
2021
Nanjing Medical University
2021
State Key Laboratory of Analytical Chemistry for Life Science
2020
Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by the histopathological pattern of usual interstitial pneumonia and associated with high mortality rate. Recently, lung resident mesenchymal stem cells (LR-MSCs) have been identified as an important contributor to myofibroblast activation in fibrosis. Macrophages are also believed play critical role However, underlying connections between LR-MSCs macrophages pathogenesis still elusive. In this study, we investigated...
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and irreversible lung disease of unknown cause. It has been reported that both resident mesenchymal stem cells (LR‐MSCs) tumor necrosis factor‐α (TNF‐α) play important roles in the development fibrosis. However, underlying connections between LR‐MSCs TNF‐α pathogenesis are still elusive. In this study, we found pro‐inflammatory cytokine transcription factor nuclear kappa B (NF‐κB) p65 subunit were upregulated bleomycin‐induced...
An emerging paradigm proposes a crucial role for lung resident mesenchymal stem cells (LR-MSCs) via fibroblastic transdifferentiation event in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Aberrant activation Wnt/β-catenin signaling occurs virtually all fibrotic diseases and is relevant to differentiation (MSCs). In vitro, by measuring protein levels several key components involved signaling, we confirmed that this pathway was activated myofibroblast LR-MSCs. Targeted inhibition...
Abstract Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive and fatal lung disease that characterized by enhanced changes in stem cell differentiation fibroblast proliferation. Resident mesenchymal cells (LR-MSCs) can undergo phenotype conversion to myofibroblasts augment extracellular matrix production, impairing function contributing fibrosis. Hedgehog Wnt signaling are developmental signal cascades play an essential role regulating embryogenesis tissue homeostasis. Recently, it...
Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease tightly correlated with aging.The pathological features of IPF include epithelial cell senescence abundant foci highly activated fibroblasts.However, the underlying mechanism between fibroblast activation remain to be elucidated.In our study, we demonstrated that Nanog, as pluripotency gene, played an essential role in fibroblasts.In progression IPF, senescent cells could contribute fibroblasts via increasing...
The alternative activation of macrophages in the lungs has been considered as a major factor promoting pulmonary fibrogenesis; however, mechanisms underlying this phenomenon are still elusive. In study, we investigated interaction between and fibrosis‐associated alveolar epithelial cells using bleomycin‐induced mouse fibrosis model coculture system. We demonstrated that fibrosis‐promoting spatially proximate to type II (ATII) cells, permissive for paracrine‐induced macrophage polarization....
Idiopathic pulmonary fibrosis (IPF) is a lethal, agnogenic interstitial lung disease with limited therapeutic options. To investigate vital genes involved in the development of IPF, we integrated and compared four expression profiles (GSE110147, GSE53845, GSE24206, GSE10667), including 87 IPF samples 40 normal samples. By reanalyzing these datasets, managed to identify 62 upregulated 20 downregulated Differentially expressed (DEGs) were analyzed by gene ontology Kyoto Encyclopedia Genes...
Abstract Inhibiting the myofibroblast differentiation of lung‐resident mesenchymal stem cells (LR‐MSCs) is a promising yet challenging approach for pulmonary fibrosis (PF) therapy. Here, micelles formed by graft copolymer multiple PEGs modified branched polyethylenimine are used delivering runt‐related transcription factor‐1 (RUNX1) small interfering RNA (siRNA) (siRUNX1) to lung, aiming inhibit LR‐MSCs. LR‐MSC targeting achieved functionalizing micelle surface with an anti‐stem‐cell...
Rationale: (Myo)fibroblasts are the ultimate effector cells responsible for production of collagen within alveolar structures, a core phenomenon in pathogenesis idiopathic pulmonary fibrosis (IPF). Although (myo)fibroblast-targeted therapy holds great promise suppressing progression IPF, its development is hindered by limited drug delivery efficacy to (myo)fibroblasts and vicious circle (myo)fibroblast activation evasion apoptosis. Methods: Here, dual small interfering RNA (siRNA)-loaded...
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, irreversible and the most common fatal interstitial lung disease, which characterized by damaged alveolar structure, massive proliferation of fibroblasts deposition extracellular matrix (ECM). While pathogenesis IPF remains unclear, it has been clearly established that excessive direct cause fibrogenesis. Numerous proliferating form fibrous foci secrete large amount ECM to aggravate process fibrosis. Tissue plasminogen activator...
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and fatal lung disease that characterized by enhanced changes in stem cell differentiation fibroblast proliferation. Lung resident mesenchymal cells (LR-MSCs) are important regulators of pathophysiological processes including tissue repair inflammation, evidence suggests this population also plays an essential role fibrosis. Our previous study demonstrated Wnt/β-catenin signaling aberrantly activated the lungs bleomycin-treated...
Idiopathic pulmonary fibrosis (IPF) is a long-term condition with an unidentified cause, and currently there are no specific treatment options available. Alveolar epithelial type II cells (AT2) constitute heterogeneous population crucial for secreting regenerative functions in the alveolus, essential maintaining lung homeostasis. However, comprehensive investigation into their cellular diversity, molecular features, clinical implications lacking. In this study, we conducted examination of...
Idiopathic pulmonary fibrosis (IPF) is a devastating lung disease of unknown cause, and the involvement fibroblasts in its pathogenesis well recognized. However, comprehensive understanding fibroblasts’ heterogeneity, their molecular characteristics, clinical relevance IPF lacking. In this study, we aimed to systematically classify fibroblast populations, uncover biological features subtypes fibrotic tissue, establish an IPF-associated, fibroblast-related predictive model for IPF. Herein,...
A previous study showed that dibutyl phthalate (DBP) exposure disrupted the growth of testicular Sertoli cells (SCs). In present study, we aimed to investigate potential mechanism by which DBP promotes juvenile SC proliferation in vivo and vitro.Timed pregnant BALB/c mice were exposed vehicle, or (50, 250, 500 mg/kg/day) from 12.5 days gestation until delivery. vitro, CCK-8 EdU incorporation assays performed determine effect monobutyl (MBP), active metabolite DBP, on TM4 cells, are a cell...
Microcystin-leucine arginine (MC-LR) is a kind of toxin produced by cyanobacterial, resulting in decrease testosterone levels serum and leading to impaired spermatogenesis. Gonadotropin-releasing hormone (GnRH) neurons play crucial roles the regulation release. Meanwhile, it has been demonstrated that MC-LR capable entering GnRH inducing apoptosis. Nevertheless, molecular mechanism induced apoptosis remains elusive. In present study, we found inhibited cell viability GT1–7 cells. addition,...