A5082337475- Bone health and osteoporosis research
- Adipose Tissue and Metabolism
- Bone Metabolism and Diseases
- Bone health and treatments
- CRISPR and Genetic Engineering
- bioluminescence and chemiluminescence research
- Vitamin D Research Studies
- MicroRNA in disease regulation
- Diet and metabolism studies
- Stress Responses and Cortisol
- Adipokines, Inflammation, and Metabolic Diseases
- Parathyroid Disorders and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Growth Hormone and Insulin-like Growth Factors
- Metabolism, Diabetes, and Cancer
- Exercise and Physiological Responses
- Pain Mechanisms and Treatments
- Bone and Joint Diseases
- Schizophrenia research and treatment
- Receptor Mechanisms and Signaling
- Regulation of Appetite and Obesity
- Thermoregulation and physiological responses
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- Dietary Effects on Health
MaineHealth
2020-2024
University of Maine
2020-2024
Tufts University
2020-2024
Maine Medical Center Research Institute
2011-2022
Maine Medical Center
2011-2022
Research Network (United States)
2020-2022
Michigan State University
2006-2015
Laboratoire d’Imagerie Biomédicale
2011
Type 1 diabetes (T1D)–induced osteoporosis is characterized by a predominant suppression of osteoblast number and activity, as well increased bone marrow adiposity but no change in osteoclast activity. The fundamental mechanisms alternative anabolic treatments (with few side effects) for T1D loss remain undetermined. Recent studies our laboratory others indicate that probiotics can benefit health. Here, we demonstrate Lactobacillus reuteri, probiotic with anti-inflammatory health properties,...
As many as 50% of adults with type I (T1) diabetes exhibit bone loss and are at increased risk for fractures. Therapeutic development to prevent and/or restore lost in T1 diabetic patients requires knowledge the molecular mechanisms accounting pathology. Because cell culture models alone cannot fully address systemic/metabolic complexity diabetes, animal critical. A variety exist including spontaneous pharmacologically induced rodents. In this paper, we discuss streptozotocin (STZ)-induced...
CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up 16% efficiency in generating conditional (cKO or floxed) alleles by microinjection 2 single guide RNAs (sgRNA) single-stranded oligonucleotides as donors (referred herein "two-donor floxing" method).We re-evaluate two-donor method from a consortium 20 laboratories across...
Abstract Type 1 diabetic osteoporosis results from impaired osteoblast activity and death. Therefore, anti‐resorptive treatments may not effectively treat bone loss in this patient population. Intermittent parathyroid hormone (PTH) treatment stimulates remodeling increases density healthy subjects. However, PTH effects be limited patients with diseases that interfere its signaling. Here, we examined the ability of 8 40 µg/kg intermittent to counteract loss. reduced fat pad mass blood glucose...
Abstract Type 2 diabetes (T2D) incidence in adolescents is rising and may interfere with peak bone mass acquisition. We tested the effects of early-onset T2D on mass, microarchitecture, strength TALLYHO/JngJ mouse, which develops by 8 weeks age. assessed metabolism skeletal acquisition male SWR/J controls (n = 8–10/group) from 4 to 17 Tallyho mice were obese; had an approximately 2-fold higher leptin percentage body fat; lower mineral density vs SWR at all time points (P < .03 for...
The difficulty in obtaining as well maintaining weight loss, together with the impairment of metabolic control conditions like diabetes and cardiovascular disease, may represent pathological situations inadequate neural communication between brain peripheral organs tissues. Innervation adipose tissues by nerves provides a means master regulator (chiefly hypothalamus), energy-expending energy-storing cells body (primarily adipocytes). Although chemical surgical denervation studies have...
ABSTRACT Fat mass may be modulated by the number of brown-like adipocytes in white adipose tissue (WAT) humans and rodents. Bone remodeling is dependent on systemic energy metabolism and, with age, bone becomes uncoupled brown (BAT) function declines. To test interaction between BAT bone, we employed Misty (m/m) mice, which were reported deficient BAT. We found that mice have accelerated age-related trabecular loss impaired fat (including reduced temperature, lower expression Pgc1a, less...
Abstract Type I diabetes is associated with bone loss and marrow adiposity. To identify early events involved in the etiology of diabetic loss, was induced mice by multiple low dose streptozotocin injections. Serum markers metabolism inflammation as well tibial gene expression were examined between 1 17 days post‐injection (dpi). At 3 dpi, when blood glucose levels significantly elevated, body, fat pad muscle mass decreased. resorption formation decreased at 5 dpi remained suppressed...
Abstract Leptin is a hormone secreted by adipocytes that implicated in the regulation of bone density. Serum leptin levels are decreased rodent models type 1 (T1‐) diabetes and diabetic patients. Whether mediates changes unclear. Therefore, we treated control T1‐diabetic mice with chronic (28 days) subcutaneous infusion or saline to elucidate therapeutic potential for osteoporosis. prevented increase marrow increased aP2 expression observed vehicle‐treated mice. However, did not prevent...
Atypical antipsychotic (AA) drugs cause significant metabolic side effects, and clinical data are emerging that demonstrate increased fracture risk bone loss after treatment with the AA, risperidone (RIS). The pharmacology underlying adverse effects on is unknown. However, RIS action in central nervous system could be responsible because sympathetic (SNS) known to uncouple remodeling. mice significantly lowered trabecular volume fraction (bone volume/total volume), owing osteoclast-mediated...
Type I diabetes is characterized by little or no insulin production and hyperglycemic conditions. It also associated with significant bone loss increased marrow adiposity. To examine the role of reduced signaling in type diabetic without inducing hyperglycemia, we used genetically reconstituted receptor knockout mice (IRKO-L1) that are euglycemic as a result human transgene expression pancreas, liver, brain. RT-PCR analyses demonstrated undetectable levels IRKO-L1 bone, yet bones exhibit...
The increasing use of antipsychotics (APs) to treat pediatric psychiatric conditions has led concerns over the long-term tolerability these drugs. While risk cardiometabolic abnormalities received most attention, preclinical and clinical studies provide preliminary evidence that APs can adversely impact bone metabolism. This would be concerning in children adolescents as suboptimal accrual during development may lead increased fracture later life. However, potential mechanisms action through...
Bone loss in type 1 diabetes is accompanied by increased marrow fat, which could directly reduce osteoblast activity or result from altered bone mesenchymal cell lineage selection (adipocyte vs. osteoblast). CCAAT/enhancer binding protein beta (C/EBPβ) an important regulator of both adipocyte and differentiation. C/EBPβ-null mice have delayed formation defective lipid accumulation brown adipose tissue. To examine the balance C/EBPβ functions diabetic context, we induced (knockout, KO) mice....
There is a growing and alarming prevalence of obesity the metabolic syndrome in type I diabetic patients (T1DM), particularly adolescence. In general, low bone mass, higher fracture risk, increased marrow adipose tissue (MAT) are features osteopathy insulin‐deficient subjects. On other hand, 2 diabetes (T2DM) associated with normal or high greater risk peripheral fractures, no change MAT. Therefore, we sought to determine effect weight gain on turnover mice. We evaluated impact 6‐week...
Trpm8 (transient receptor potential cation channel, subfamily M, member 8) is expressed by sensory neurons and involved in the detection of environmental cold temperatures. TRPM8 activity triggers an increase uncoupling protein 1 ( Ucp1 )-dependent brown adipose tissue (BAT) thermogenesis. Bone density marrow are both influenced rodent housing temperature tissue, but it unknown if co-regulation thermogenesis bone homeostasis. To address this, we examined phenotypes one-year-old knockout mice...