A5031923567- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- Advanced Fluorescence Microscopy Techniques
- Genetics, Aging, and Longevity in Model Organisms
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Hereditary Neurological Disorders
- 3D Printing in Biomedical Research
- Sirtuins and Resveratrol in Medicine
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Genetics and Physical Performance
- Diet and metabolism studies
- Telomeres, Telomerase, and Senescence
- Muscle metabolism and nutrition
- Botulinum Toxin and Related Neurological Disorders
- Thermoregulation and physiological responses
- Hormonal and reproductive studies
- Single-cell and spatial transcriptomics
Neurological Surgery
2019-2024
The Ohio State University
2019-2024
The Ohio State University Wexner Medical Center
2022-2023
University of Maine
2018-2023
Peripheral neuropathy, which can include axonal degeneration and/or demyelination, impacts adipose tissues with obesity, diabetes, and aging. However, the presence of demyelinating neuropathy had not yet been explored in adipose. Both neuropathies axonopathies implicate Schwann cells (SCs), a glial support cell that myelinates axons contributes to nerve regeneration after injury. We performed comprehensive assessment SCs myelination patterns subcutaneous white tissue (scWAT) nerves, changes...
The difficulty in obtaining as well maintaining weight loss, together with the impairment of metabolic control conditions like diabetes and cardiovascular disease, may represent pathological situations inadequate neural communication between brain peripheral organs tissues. Innervation adipose tissues by nerves provides a means master regulator (chiefly hypothalamus), energy-expending energy-storing cells body (primarily adipocytes). Although chemical surgical denervation studies have...
Abstract Neural communication between the brain and adipose tissues regulates energy expenditure metabolism through modulation of tissue functions. We have recently demonstrated that under pathophysiological conditions (obesity, diabetes, aging), total subcutaneous white (scWAT) innervation is decreased (‘adipose neuropathy’). With advanced age in C57BL/6J mouse, small fiber peripheral nerve endings die back, resulting reduced contact with adipose‐resident blood vessels other cells. This...
The sympathetic nervous system (SNS) is a crucial arm of the peripheral (PNS) and includes catecholaminergic neurons that release norepinephrine (NE) onto numerous effector tissues organs in body. SNS innervation both white (WAT) brown adipose tissue (BAT) clearly essential for proper function metabolic control, as decades surgical, chemical, genetic denervation studies have demonstrated. Despite our vast knowledge about innervation, especially context cold-stimulated browning thermogenesis...
Abstract Background Innervation of adipose tissue is essential for the proper function this critical metabolic organ. Numerous surgical and chemical denervation studies have demonstrated how maintenance brain-adipose communication through both sympathetic efferent sensory afferent nerves helps regulate adipocyte size, cell number, lipolysis, ‘browning’ white tissue. Neurotrophic factors are growth that promote neuron survival, regeneration, plasticity, including neurite outgrowth synapse...
Little is known about the diversity and function of adipose tissue nerves, due in part to inability effectively visualize tissue's diverse nerve subtypes patterns innervation across an intact depot. The tools image quantify are currently limited. Here, we present a method processing that decreases thickness z-axis while leaving cells for subsequent immunostaining. This was combined with autofluorescence quenching techniques permit whole tissues be mounted on slides imaged by confocal...
Peripheral nerves allow a bidirectional communication between brain and adipose tissues, many studies have clearly demonstrated that loss of the nerve supply results in tissue dysfunction metabolic dysregulation. Neuroimmune cells closely associate with including subcutaneous white (scWAT). However, scWAT, their functions beyond degrading norepinephrine an obese state remain largely unexplored. We previously reported myeloid-lineage knockout (KO) brain-derived neurotrophic factor (BDNF)...
We assessed aging hallmarks in skin, muscle, and adipose the genetically diverse HET3 mouse, generated a broad dataset comparing these to individual animal diagnostic SNPs from 4 founding inbred strains of line. For middle- old-aged mice, we provided running wheel exercise ensure our observations were not purely representative sedentary animals, but age-related phenotypes improved with activity. Adipose tissue fibrosis, peripheral neuropathy, loss neuromuscular junction integrity consistent...
Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) immunofluorescence as an alternative to current clearing-based approaches. We use combination of Z-depth reduction and autofluorescence quenching techniques fluorescently label, image, quantify innervation effectively throughout intact mouse tissues without the need optical clearing or light sheet microscopy. This has been optimized validated neurovascular labeling. For...
White adipose tissue (WAT) is a dynamic organ capable of remodelling in response to metabolic state. For example, stimuli such as cold exposure, WAT can develop inducible brown adipocytes ('browning') non-shivering thermogenesis, through concurrent changes mitochondrial content and function. This aided by increased neurite outgrowth angiogenesis across the tissue, providing needed neurovascular supply for uncoupling protein 1 activation. While several RNA-sequencing studies have been...
Abstract The difficulty in obtaining as well maintaining weight loss, together with the loss of metabolic control conditions like diabetes and cardiovascular disease, may represent pathological situations inadequate neural communication between brain peripheral organs tissues. Innervation adipose tissues by nerves provides a means master regulator (chiefly hypothalamus), energy-expending energy-storing cells body (primarily adipocytes). Although chemical surgical denervation studies have...
Summary Little is known about the diversity and function of adipose tissue nerves due, in part, to inability effectively visualize various nerve subtypes residing within these tissues. The tools currently available for researchers image quantify innervation are limited dependent on optical clearing techniques light sheet microscopy. Here we present a method processing that uses mechanical force compress decrease thickness z-axis by expanding it x y-axes whilst leaving cells intact. This has...
Abstract Telomerase reverse transcriptase (TERT) is expressed by quiescent adult stem cells (ASC) in numerous murine and human tissues, but has never been explored the brain. Here, we demonstrate that TERT+ mouse brain represent a novel population of multipotent ASCs are localized to classical neuro/gliogenic niches (including ventricular-subventricular zone, hypothalamus, olfactory bulb), as well more recently described regions plasticity such meninges choroid plexus. Using direct-reporter...
Abstract Background Innervation of adipose tissue is essential for the proper function this critical metabolic organ. Numerous surgical and chemical denervation studies have demonstrated how maintenance brain-adipose communication through both sympathetic efferent sensory afferent nerves helps regulate adipocyte size, cell number, lipolysis, ‘browning’ white tissue. Neurotrophic factors are growth that promote neuron survival, regeneration plasticity, including neurite outgrowth synapse...
Abstract Background: Innervation of adipose tissue is essential for the proper function this critical metabolic organ. Numerous surgical and chemical denervation studies have demonstrated how maintenance brain-adipose communication through both sympathetic efferent sensory afferent nerves helps regulate adipocyte size, cell number, lipolysis, ‘browning’ white tissue. Neurotrophic factors are growth that promote neuron survival, regeneration plasticity, including neurite outgrowth synapse...
ABSTRACT Peripheral neuropathy is a pathophysiological state of nerve degeneration and loss tissue innervation. The most prominent cause small fiber diabetes which can be demyelinating in nature, but this has not yet been explored adipose tissue. Both neuropathies axonopathies implicate Schwann cells (SCs), the peripheral glial required for myelination regeneration after injury. Here, we perform comprehensive assessment SCs patterns subcutaneous white (scWAT) nerves, including changes that...
Cover legend: The cover image is based on the Research Article Age-related changes to adipose tissue and peripheral neuropathy in genetically diverse HET3 mice differ by sex are not mitigated rapamycin longevity treatment Jake W. Willows et al., https://doi.org/10.1111/acel.13784.
Abstract The sympathetic nervous system (SNS) is a crucial arm of the peripheral (PNS) and includes catecholaminergic neurons that release norepinephrine (NE) onto numerous effector tissues organs in body. SNS innervation both white (WAT) brown adipose tissue (BAT) clearly essential for proper function metabolic control, as decades surgical, chemical, genetic denervation studies have demonstrated. Despite our vast knowledge about innervation, especially context cold‐stimulated browning...
Abstract The difficulty in obtaining as well maintaining weight loss, together with the impairment of metabolic control conditions like diabetes and cardiovascular disease, may represent pathological situations inadequate neural communication between brain peripheral organs tissues. Innervation adipose tissues by nerves provides a means master regulator (chiefly hypothalamus), energy-expending energy-storing cells body (primarily adipocytes). Although chemical surgical denervation studies...
Abstract Background: Innervation of adipose tissue is essential for the proper function this critical metabolic organ. Numerous surgical and chemical denervation studies have demonstrated how maintenance brain-adipose communication through both sympathetic efferent sensory afferent nerves help regulate adipocyte size, cell number, lipolysis, ‘browning’ white tissue. Neurotrophic factors are growth that promote neuron survival, regeneration, plasticity, including neurite outgrowth synapse...