A5007406387- Muscle Physiology and Disorders
- Acute Ischemic Stroke Management
- Adipose Tissue and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Stroke Rehabilitation and Recovery
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Botulinum Toxin and Related Neurological Disorders
- Angiogenesis and VEGF in Cancer
- Muscle activation and electromyography studies
- Body Composition Measurement Techniques
- Cerebrovascular and Carotid Artery Diseases
- Protease and Inhibitor Mechanisms
- Mesenchymal stem cell research
- Phytoestrogen effects and research
- Electron Spin Resonance Studies
- Cerebral Palsy and Movement Disorders
- Barrier Structure and Function Studies
- Calcium signaling and nucleotide metabolism
- Muscle metabolism and nutrition
- Hereditary Neurological Disorders
- Planarian Biology and Electrostimulation
- Bone fractures and treatments
- Biochemical effects in animals
- Natural Antidiabetic Agents Studies
The Ohio State University
2017-2025
The Ohio State University Wexner Medical Center
2016-2025
Ohio University
2023
University of Missouri
2023
Royal Navy
1964
United States Army Reserve
1964
Fibroblasts nonvirally programmed to convert into induced endothelial cells show therapeutic potential for ischemic stroke.
Ischemic stroke results in excessive release of glutamate, which contributes to neuronal cell death. Here, we test the hypothesis that otherwise neurotoxic glutamate can be productively metabolized by oxaloacetate transaminase (GOT) maintain cellular energetics and protect brain from ischemic injury. The GOT-dependent metabolism was studied primary neural cells stroke-affected C57-BL6 mice using magnetic resonance spectroscopy GC-MS. Extracellular Glu sustained viability under hypoglycemic...
In the pathophysiologic setting of cerebral ischemia, excitotoxic levels glutamate contribute to neuronal cell death. Our previous work demonstrated ability oxaloacetate transaminase (GOT) metabolize neurotoxic in stroke-affected brain. Here, we seek identify small-molecule inducers GOT expression mitigate ischemic stroke injury. From a panel phytoestrogen isoflavones, biochanin A (BCA) was identified as most potent inducer gene neural cells. BCA significantly increased mRNA and protein at...
Kinesin family member 5A (KIF5A) is an essential, neuron-specific microtubule-associated motor protein responsible for the anterograde axonal transport of various cellular cargos. Loss function variants in N-terminal, microtubule-binding domain are associated with hereditary spastic paraplegia and neuropathy. These result a loss ability mutant to process along microtubules. Contrastingly, gain splice-site C-terminal, cargo-binding KIF5A amyotrophic lateral sclerosis (ALS), neurodegenerative...
The efficacy and optimization of poststroke physical therapy paradigms is challenged in part by a lack objective tools available to researchers for systematic preclinical testing. This work represents maiden effort develop robot-assisted mechanical (RAMT) device objectively address the significance physiotherapy on outcomes. Wistar rats were subjected right hemisphere middle-cerebral artery occlusion reperfusion. After 24 h, split into control (RAMT−)or RAMT+ groups (30 min daily RAMT over...
We assessed aging hallmarks in skin, muscle, and adipose the genetically diverse HET3 mouse, generated a broad dataset comparing these to individual animal diagnostic SNPs from 4 founding inbred strains of line. For middle- old-aged mice, we provided running wheel exercise ensure our observations were not purely representative sedentary animals, but age-related phenotypes improved with activity. Adipose tissue fibrosis, peripheral neuropathy, loss neuromuscular junction integrity consistent...
Abstract While gene and cell therapies have emerged as promising treatment strategies for various neurological conditions, heavy reliance on viral vectors can hamper widespread clinical implementation. Here, the use of tissue nanotransfection a platform nanotechnology to drive nonviral delivery nerve via nanochannels, in an effective, controlled, benign manner is explored. TNT facilitates plasmid DNA sciatic mice voltage‐dependent manner. Compared standard bulk electroporation (BEP),...
Ischemic stroke is a leading cause of mortality and chronic disability worldwide, underscoring the need for reliable accurate animal models to study this disease's pathology, molecular mechanisms injury, treatment approaches. As most clinical strokes occur in regions supplied by middle cerebral artery (MCA), several experimental have been developed simulate an MCA occlusion (MCAO), including transcranial MCAO, micro- or macro-sphere embolism, thromboembolisation, photothrombosis,...
Pathological, age-related loss of muscle function, commonly referred to as sarcopenia, contributes mobility, impaired independence, well increased risk adverse health events. Sarcopenia has been attributed changes in both neural and muscular integrity during aging. Current treatment options are primarily limited exercise dietary protein fortification, but the therapeutic impact these approaches often inadequate. Prior work suggested that a ketogenic diet (KD) might improve healthspan...
Purpose: Peripheral nerve injuries (PNIs) can have lasting consequences, including sensory/motor deficits and muscle loss, leading to significant morbidity disability. While gene cell therapies shown potential improve outcomes after PNIs, there is a lack of technologies facilitate their translation strategies mitigate progressive degeneration. Here we describe the use novel technology, Tissue Nano-Transfection (TNT), that deliver multi-pronged gene/cell nerve/muscle aimed at improving rate...
Introduction: While recombinant tissue plasminogen activator (rTPA) is the mainstay of ischemic stroke treatment, recanalization only achieved in 25-50% patients. With a significant risk intracranial hemorrhage, its use has been limited to within 4.5 hours symptom onset. Previous work demonstrated that aptamer inhibition Von Willebrand Factor (VWF) effectively restores reperfusion following murine carotid artery occlusion. Hypothesis: We tested hypothesis VWF would promote thrombotic middle...
Abstract Objective Pathological, age-related loss of muscle function, commonly referred to as sarcopenia, contributes mobility, impaired independence, well increased risk adverse health events. Sarcopenia has been attributed changes in both neural and muscular integrity during aging. Current treatment options are primarily limited exercise dietary protein fortification, but the therapeutic impact these approaches often inadequate. Prior work suggested that a ketogenic diet (KD) might improve...
Introduction: A growing body of literature supports acute changes to skeletal muscle physiology in response stroke-induced central nervous system injury. While stroke survivors depend on rehabilitation facilitate functional recovery, study post-stroke and mechanisms recovery remains limited. The current work addresses development a Robot-Assisted Mechanical Therapy (RAMT) device facilitation reproducible, objective analysis rehabilitation. We hypothesize that RAMT permits systematic...
Introduction: While recombinant tissue plasminogen activator (rTPA) is the mainstay of ischemic stroke treatment, few patients are eligible for and recanalization only seen in 25-50%. Von Willebrand Factor (VWF) inhibition may play a role thrombolysis. Hypothesis: VWF with an RNA aptamer lyses arterial thrombus decreases injury. Furthermore, reversal antidote oligonucleotide ameliorates intracranial hemorrhage (ICH). Methods: Adult wild-type (C57BL/6J) mice were anesthetized, right carotid...
Introduction: While recombinant tissue plasminogen activator (rTPA) is the mainstay of ischemic stroke treatment, few patients are eligible for and recanalization only seen in 25-50%. Von Willebrand Factor (VWF) inhibition may play a role thrombolysis. Hypothesis: VWF with an RNA aptamer lyses arterial thrombus decreases injury. Furthermore, reversal antidote oligonucleotide ameliorates intracranial hemorrhage (ICH). Methods: Adult wild-type (C57BL/6J) mice were anesthetized, right carotid...
Introduction: Endothelial dysfunction is an important mediator of post ischemic injury the heart and brain following ischemia/reperfusion (I/R). We have reported that CD38 activation in I/R models leads to NADPH depletion with endothelial nitric oxide synthase (eNOS) impairment, loss endothelial-mediated coronary dilatation increased myocardial infarction. While knockout or inhibition prevents this decreases infarction, role stroke remains uncertain. Hypothesis: hypothesize expression...
There is growing evidence that loss of muscle function during age related to strength (Dynapenia) and motor performance. Therapeutic role a ketogenic diet (KD) in increasing lifespan aged mice, KD appears convey positive impact on the context age-related sarcopenia. PURPOSE: To investigate effects physical mice determine effect intervention METHODS: Aged (22 months) C57BL/6 were assessed at baseline then stratified (normocaloric) (n = 22, 13 female) or regular chow 15, 7 ad libitum....
To investigate the impact of electrode type and high pass filter settings on compound muscle action potential (CMAP) recordings in mouse models with goal to optimize preclinical approaches.
Sarcopenia, or age-related loss of muscle mass and strength, is an important contributor to physical function in older adults. Several pathophysiological mechanisms have been proposed contribute sarcopenia, but metabolic syndrome obesity are potential risk factor for sarcopenia. PURPOSE: The purpose this study was investigate the effects a high-fat diet (HFD) on neuromuscular integrity aged rat model electrical impedance myography as biomarker status. METHODS: Middle-aged (15 months) female...