A5064829603- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Cancer-related molecular mechanisms research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Cell Image Analysis Techniques
- Pancreatic function and diabetes
- Gastric Cancer Management and Outcomes
- Pancreatic and Hepatic Oncology Research
- RNA Interference and Gene Delivery
- bioluminescence and chemiluminescence research
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- Amino Acid Enzymes and Metabolism
- Colorectal and Anal Carcinomas
- Polyamine Metabolism and Applications
National Institute of Advanced Industrial Science and Technology
2023-2024
Osaka University
2014-2023
Tokyo University of Science
2021-2023
National Center for Global Health and Medicine
2010-2015
Japan Society of Chemotherapy
2015
OncoPlex Diagnostics (United States)
2012
Alberta Health Services
2012
University of Calgary
2012
Moffitt Cancer Center
2012
Monash University
2012
N6-methyladenosine (m6A) is the most abundant epitranscriptome modification in mammalian mRNA. Recent years have seen substantial progress m6A epitranscriptomics, indicating its crucial roles initiation and progression of cancer through regulation RNA stabilities, mRNA splicing, microRNA processing translation. However, by what means dynamically regulated or written enzymatic components represented methyltransferase-like 3 (METTL3) how significant for each numerous genes remain unclear. We...
// Yujiro Nishizawa 1, 2, * , Masamitsu Konno 3, Ayumu Asai 3 Jun Koseki Koichi Kawamoto 2 Norikatsu Miyoshi 1 Hidekazu Takahashi Naohiro Nishida Naotsugu Haraguchi Daisuke Sakai Toshihiro Kudo Taishi Hata Chu Matsuda Tsunekazu Mizushima 4 Taroh Satoh Yuichiro Doki Masaki Mori and Hideshi Ishii Department of Gastroenterological Surgery, Graduate School Medicine, Osaka University, Suita, Osaka, Japan Frontier Science for Cancer Chemotherapy, Disease Data Science, Therapeutics Inflammatory...
Pyruvate kinase M2 (PKM2) is an alternatively spliced variant of the pyruvate gene that preferentially expressed during embryonic development and in cancer cells. PKM2 alters final rate-limiting step glycolysis, resulting cancer-specific Warburg effect (also referred to as aerobic glycolysis). Although previous reports suggest functions nonmetabolic transcriptional regulation, its significance biology remains elusive. Here we report stimulation epithelial-mesenchymal transition (EMT) results...
Abstract The biological significance of micro (mi)RNAs has traditionally been evaluated according to their RNA expression levels based on the assumption that miRNAs recognize and regulate targets in an unvarying fashion. Here we show a fraction mature including miR-17-5p, -21-5p, -200c-3p let-7a-5p harbor methyl marks potentially alter stability target recognition. Importantly, methylation these was significantly increased cancer tissues as compared paired normal tissues. Furthermore,...
SIRT4, which is localised in the mitochondria, one of least characterised members sirtuin family nicotinamide adenine dinucleotide-dependent enzymes that play key roles multiple cellular processes such as metabolism, stress response and longevity. There are only a few studies have its function assessed clinical significance human cancers. We established colorectal cancer cell lines (SW480, HCT116, HT29) overexpressing SIRT4 investigated their effects on proliferation, migration invasion,...
Pancreatic cancer has a poor prognosis because of its high refractoriness to chemotherapy and tumour recurrence, these properties have been attributed stem cells (CSCs). MicroRNA (miRNA) regulates various molecular mechanisms progression associated with CSCs. This study aimed identify the candidate miRNA characterise clinical significance. We established gemcitabine-resistant Panc1 cells, induced CSC-like through sphere formation. Candidate miRNAs were selected microarray analysis. The...
Abstract Deranged metabolism is a hallmark of cancer, playing significant role in driving the disease process. One such example induction carcinogenesis by oncometabolite D-2 hydroxyglutarate (D-2HG), which produced mutated enzyme isocitrate dehydrogenase ( IDH ) occurring subsets leukaemias and brain tumours. The oncogenic property D-2HG appears to stem from its ability interfere with activities α-ketoglutarate-dependent dioxygenases, including Jumonji family histone demethylases. Here, we...
CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up 16% efficiency in generating conditional (cKO or floxed) alleles by microinjection 2 single guide RNAs (sgRNA) single-stranded oligonucleotides as donors (referred herein "two-donor floxing" method).We re-evaluate two-donor method from a consortium 20 laboratories across...
Cancer stem cells (CSCs) are known to influence chemoresistance, survival, relapse and metastasis. Aldehyde dehydrogenase (ALDH) functions as an epithelial CSC marker. In the present study, we investigated involvement of ALDH in gastric maintenance, chemoresistance survival. Following screening for eight candidate markers (CD13, CD26, CD44, CD90, CD117, CD133, EpCAM ALDH), five cancer cell lines were found contain small subpopulations high activity (ALDHhigh cells). We also examined ALDHhigh...
Cancer stem cells (CSCs) are responsible for treatment failure. However, their identification and roles in resistance not well established head neck squamous cell carcinoma (HNSCC). Three HNSCC lines (FaDu, Detroit562 BICR6) were treated with cisplatin or radiation. Cell surface antigens analysed by LyoPlate, a novel antigen array. The expression levels of highly expressed after treatments further compared between cisplatin-resistant its parental line. Association the candidate CSCs...
Abstract Polypyrimidine tract–binding protein (PTBP1) is an RNA-binding with various molecular functions related to RNA metabolism and a major repressive regulator of alternative splicing, causing exon skipping in numerous alternatively spliced pre-mRNAs. Here, we have investigated the role PTBP1 colorectal cancer. expression levels were significantly overexpressed cancerous tissues compared corresponding normal mucosal tissues. We also observed that levels, c-MYC PKM2:PKM1 ratio positively...
Abstract Tumor cells respond to their microenvironment, which can include hypoxia and malnutrition, adapt metabolism survive grow. Some oncogenes are associated with cancer via regulation of the related enzymes or transporters. However, importance precise metabolic effects in colorectal remain unclear. We found that survived under condition glucose depletion, resistance such conditions depended on genomic alterations rather than KRAS mutation alone. Metabolomic analysis demonstrated those...
Abstract Cancer stem cells (CSCs) are involved in metastatic colorectal cancer recurrence, but no effective therapy targeting these is currently available. Because trifluridine (FTD)/tipiracil used for refractory cancer, we sought to determine whether FTD against CSC-like cells. CD44 + CD133 high-expressing and other populations of human DLD-1 colon were separately isolated through fluorescence-activated cell sorting. The sphere-forming activity each population the anti-sphere-forming...
Abstract Background Tissue-resident memory T (Trm) cells are associated with cytotoxicity not only in viral infection and autoimmune disease pathologies but also many cancers. Tumour-infiltrating CD103 + Trm predominantly comprise CD8 that express cytotoxic activation immune checkpoint molecules called exhausted markers. This study aimed to investigate the role of colorectal cancer (CRC) characterise cancer-specific Trm. Methods Immunochemical staining anti-CD8 anti-CD103 antibodies for...
Metabolism may determine the biologically malignant behavior of pancreatic cancer. To investigate significance and prognostic value cancer metabolism in patients, we investigated expression two key enzymes anaerobic glycolysis, hexokinase 2 (HK2) pyruvate kinase isoenzyme type M2 (PKM2), surgical specimens obtained from 36 patients who underwent curative resection ductal carcinoma. The hk2-glycolysis axis is a system clinical imaging tumors via positron emission tomography....
The global incidence of colorectal cancer (CRC) is increasing. Although there are emerging epigenetic factors that contribute to the occurrence, development and metastasis CRC, biological significance molecular regulation in different subpopulations such as stem cells remains be elucidated. In this study, we investigated functional roles H3K4 demethylase, jumonji, AT rich interactive domain 1B (JARID1B), an factor required for continuous cell growth melanomas, CRC. We found CD44(+)/aldehyde...
The expression levels of one-carbon metabolic enzymes were investigated and observed to be correlated with clinicopathological parameters in patients pancreatic cancer. Mitochondrial metabolism comprises a network biological reactions that integrate nutrient status nucleotide synthesis, amino acid metabolism, antioxidant reduced nicotinamide adenine dinucleotide phosphate production epigenetic methylation processes. Previous studies have reported the hyper-activation mitochondrial serves...
The significance of mitochondrial metabolism in cancer cells has recently been gaining attention. Among other findings, One-carbon folate reported to be closely associated with cellular characteristics cancer. To study molecular targets for efficient therapy, we investigated the association between expressions genes that code enzymes involved one-carbon and survival rate patients adenocarcinomas colorectum lung. Patients high expression control metabolic cycle tetrahydrofolate (THF)...
Abstract Although BRAF V600E mutation is associated with adverse clinical outcomes in patients colorectal cancer (CRC), response and resistance mechanisms for therapeutic inhibitors remains poorly understood. In the present study, we demonstrate that selective inhibition activates AMP-activated protein kinase (AMPK), which induces autophagy as a mechanism of human cancers. The data show AMPK-dependent cytoprotective roles under conditions AMPK was negatively correlated -dependent activation...