A5059300652- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Viral Infections and Immunology Research
- Asthma and respiratory diseases
- Insects and Parasite Interactions
- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
- Insect and Pesticide Research
- RNA and protein synthesis mechanisms
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Dermatological diseases and infestations
- Respiratory viral infections research
- Dermatology and Skin Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Spaceflight effects on biology
- interferon and immune responses
- vaccines and immunoinformatics approaches
Medical University of Vienna
2013-2024
Luxembourg Institute of Health
2024
Hospital Clínic de Barcelona
2024
Ludwig Boltzmann Institute Applied Diagnostics
2023
Vienna Biocenter
2015-2018
Max Perutz Labs
2013-2018
Significance An understanding of the mechanisms by which viruses evade host immunity is essential to development antiviral drugs and viral detection strategies. Ubiquitin ubiquitin-like modifications are crucial in cellular innate immune infection responses often suppressed proteins. We here identify a previously unknown mechanism evasion. A protease, Lb pro , removes ubiquitin protein ISG15 incompletely from While this strategy efficiently irreversibly shuts down these modification systems,...
Background: Many fish-allergic patients only react to certain fish species and may tolerate others, mostly because of IgE-mediated recognition specific epitopes on the major allergen parvalbumin. However, considerable number consumed makes it challenging identify which are allergenic tolerated by individual patients. Objective: In order improve diagnosis allergy, we investigated reactivity parvalbumins from 12 freshwater that largely underrepresented in diagnostic tests. Methods:...
Detecting IgE sensitizations in the serum of allergic dogs is commonly done using allergen extracts, but these are difficult to standardize. This article details development and validation Pet Allergy Xplorer (PAX; Nextmune, Stockholm, Sweden), first multiplex macroarray for detection sensitization extracts molecular components; PAX derived from (ALEX2; MacroArray Diagnostics, Vienna, Austria). The selection allergens, cartridge processing, strategy identifying blocking directed against...
Abstract Background Immunoglobulin (Ig)E cross‐reactivity has been shown between Dermatophagoides farinae (Df; house dust mite) and the nematode Toxocara canis (Tc), yet its allergen basis is unknown. Objective s To identify Df allergens IgE‐cross‐reactive with those of Tc. Animals Archived sera from 73 dogs suspected allergy sensitised to Df. Materials Methods We performed a combination Pet Allergy Xplorer (PAX) enzyme‐linked immunosorbent assay (ELISA) inhibitions excretory–secretory...
Abstract Background The specificity of extract‐based pollen allergy diagnosis is decreased due to cross‐reactivity via cross‐reactive carbohydrate determinants (CCDs) or panallergens such as profilins polcalcins. This study aimed explore the prevalence sensitization seasonal extracts, CCDs, profilin and polcalcin investigate sensitivity molecular (MAD) using commercially available test methods. Methods 2948 patients were screened for specific immunoglobulin E ash, birch, mugwort, ragweed...
In enteroviruses, the inhibition of protein synthesis from capped host cell mRNA is catalyzed by virally encoded 2A proteinase (2Apro), which cleaves eukaryotic initiation factors (eIF) 4GI and 4GII. Despite much investigation, exact mechanism 2Apro cleavage remains however unclear. Here, we identify domains responsible for eIF4E/HRV2 interaction using molecular modelling describe mutations that impair this delay in vitro eIF4G isoforms. Furthermore, produced HRV1A viruses bearing mutation...
<b><i>Background:</i></b> The association among the IgE responses to prevailing groups of house dust mite (HDM) allergens in concurrent asthma phenotypes has not been determined. <b><i>Objective:</i></b> aim present study lays on a component-resolved diagnosis (CRD) model investigate molecular signature subjects with type-2 inflammation asthma. <b><i>Methods:</i></b> We selected patients showing clinically relevant...
Detecting IgE sensitizations in the serum of allergic dogs is commonly performed using allergen extracts, but these are difficult to standardize. This article details development and validation Pet Allergy Xplorer (PAX; Nextmune, Stockholm, Sweden), first multiplex macroarray for detection sensitization extracts molecular components; PAX derived from (ALEX
Messenger RNA is recruited to the eukaryotic ribosome by a complex including initiation factor (eIF) 4E (the cap-binding protein), scaffold protein eIF4G and helicase eIF4A. To shut-off host-cell synthesis, cleaved during picornaviral infection virally encoded proteinase; structural basis of this reaction its stimulation eIF4E unclear. We have structurally biochemically investigated interaction purified foot-and-mouth disease virus (FMDV) leader proteinase (Lb(pro)), human rhinovirus 2...
The 2A proteinase (2A(pro)) of human rhinoviruses cleaves the virally encoded polyprotein between C terminus VP1 and its own N terminus. Poor understanding 2A(pro) substrate specificity this enzyme has hampered progress in developing inhibitors that may serve as antiviral agents. We show here rhinovirus (HRV) 1A 2 (rhinoviruses from genetic group A) cannot self-process at HRV14 (a B rhinovirus) cleavage site. When amino acids site HRV2 (Ile-Ile-Thr-Thr-Ala*Gly-Pro-Ser-Asp) were singly or...
(1) Background: After spending a year wintering in Antarctica, individual expedition members have reported increased or even new allergic reactions to environmental allergens after their return. (2) Methods: Blood samples from five overwintering crews were analyzed using the chip based multiplex ALEX Allergy Explorer (MacroArray Diagnostics GmbH, Austria). (3) Results: About one third of 39 participants displayed specific IgEs against pollen. In most individuals, kinetics showed reduction...
The prevalence of cockroach (CR) sensitization and its relevance as a trigger allergy symptoms differs greatly in different geographic areas.This study aimed to compare molecular IgE reactivity profiles CR-sensitized patients with perennial from Hong Kong (HK) Austria identify the main primary sensitizers.IgE was assessed by skin prick test and/or CR extract. Molecular were analyzed via multiplex assay for allergens extracts CR, house dust mite (HDM), shellfish, 3 additional insect...
Background: Recent studies indicated that fish-allergic patients may safely consume certain fish species. Multiplex IgE testing facilitates the identification of species tolerated by individual patients. Methods: Sera were collected from 263 Austria, China, Denmark, Luxembourg, Norway and Spain. Specific (s) to parvalbumins (PVs) 10 along with 7 raw 6 heated extracts was quantified using a research version ALEX assay. IgE-signatures patient groups analyzed SPSS R. Results: sIgE alpha-PV ray,...