Diabetic nephropathy (DN), as a chronic inflammatory complication of diabetes, is characterized by hyperglycemia, albuminuria and edema, which ultimately becomes the leading cause end-stage renal disease (ESRD). Astragalus polysaccharide (APS), extracted from membranaceus, was widely used in treatment diabetes mellitus. However, functional roles APS ameliorate responses DN, remain poorly understood. Therefore, purpose this study to explore molecular mechanism on DN vivo vitro models. We...

Diabetic nephropathy (DN), characterized by hyperglycemia, hypertension, proteinuria, and edema, is a unique microvascular complication of diabetes. Traditional Chinese medicine (TCM) Astragalus membranaceus (AM) has been widely used for DN in China while the pharmacological mechanisms are still unclear. This work aimed at undertaking network pharmacology analysis to reveal mechanism effects AM DN. Materials Methods. In this study, chemical constituents were obtained via Medicine Systems...

Salvianolic acid B (Sal B) is a potential cytotoxic polyphenol against cancer. In the present study effect of Sal and its molecular mechanism were investigated in non‑small cell lung cancer (NSCLC) A549 line. The TGF‑β/MAPK/Smad signaling axis was explored. cells co‑cultured with without different concentrations (25, 50 100 µM respectively) TGF‑β1 (9 pM) for 24 h. Cell epithelial‑mesenchymal transition (EMT), migration, cycle distribution, autophagy apoptosis assessed by western blotting...

Diabetic nephropathy (DN) is one of the common chronic complications diabetes with unclear molecular mechanisms, which associated end-stage renal disease (ESRD) and kidney (CKD). Our study intended to construct a competing endogenous RNA (ceRNA) network via bioinformatics analysis determine potential mechanisms DN pathogenesis. The microarray datasets (GSE30122 GSE30529) were downloaded from Gene Expression Omnibus database find differentially expressed genes (DEGs). GSE51674 GSE155188 used...

The Stephania tetrandra−Astragalus membranaceus herbal pair (FH) is a classic widely used in the treatment of nephrotic syndrome (NS). effects tetrandra (FJ) and Astragalus (HQ) on NS have been reported, but mechanism their combination improvement are still unclear. model was established by injecting adriamycin into tail vein. FH intervention reduced levels serum triglyceride, total cholesterol, interleukin-6 (IL-6), blood urea nitrogen (BUN), urinary protein, gene expression aquaporin 2...

A high-performance liquid chromatographic method for the determination and pharmacokinetic study of mangiferin in plasma rats that have been orally administered traditional Chinese medicinal preparation Zi-Shen pill is established. Plasma samples taken from are pretreated by protein precipitation with acetonitrile. Separation main effective constituent accomplished on a C18 stationary phase mobile methanol&-water (25:75, v/v) 0.6% glacial acetic acid. The UV detection wavelength set at 320...

To explore the pathogenesis of rheumatoid arthritis (RA) from perspective metabolomics, gas chromatography time-of-flight mass spectrometry (GC-TOF/MS) technology was used to observe changes in metabolic profiles urine output rats with adjuvant-induced (AA). Sprague-Dawley were randomly divided into a control group and an experimental group, eight each. Rats induced by intracutaneous innoculation 0.1 mL Freund's complete adjuvant right paws. On day 20 after immunization, between rat groups...

Type 2 diabetes mellitus (T2DM) has become a chronic disease, serious harm to human health. Complications of the blood pipe are main cause disability and death in diabetic patients, including vascular lesions that directly affects prognosis patients with survival. This study was determine influence high glucose related mechanism lesion type pathogenesis. In vivo aorta abdominalis GK rats observed pressure, heart rate, hematoxylin eosin (H&E), Masson, Verhoeff staining. vitro cells were...

Background: Chronic kidney disease (CKD), characterized by sustained inflammation and immune dysfunction, is highly prevalent can eventually progress to end-stage disease. However, there still a lack of effective reliable diagnostic markers therapeutic targets for CKD. Methods: First, we merged data from GEO microarrays (GSE104948 GSE116626) identify differentially expressed genes (DEGs) in CKD healthy patient samples. Then, conducted GO, KEGG, HPO, WGCNA analyses explore potential functions...

The aim of the present study was to explore changes in urinary metabolic spectrum rats with knee osteoarthritis, using gas chromatography-time flight/mass spectrometry (GC-TOF/MS) determine metabonomic disease pathogenesis. Sprague-Dawley were randomly divided into control and model groups (n=8/group), 20 µl 4% papain 0.03 M L-cysteine injected right on days 1, 3 7 establish osteoarthritis model. Following 14 days, urine collected over 12 h cartilage ultrastructural damage assessed by...

Asthma, a common but poorly controlled disease, is one of the most serious health problems worldwide; however, mechanisms underlying development asthma remain unknown. Long non‑coding RNAs (lncRNAs) and mRNAs serve important roles in initiation progression various diseases. The present study aimed to investigate role differentially expressed lncRNAs associated with asthma. Differentially were screened between expression data 62 patients 43 healthy controls. Gene ontology (GO) Kyoto...

Context Qi Teng Xiao Zhuo granule (QTXZG) is a traditional Chinese medicine (TCM) used for therapeutic effects on chronic glomerulonephritis (CGN). However, the underlying mechanism remains unclear.Objective To investigate molecular of QTXZG CGN by proteomics.Materials and methods The model was induced in Sprague–Dawley rats injecting adriamycin (3.5 mg/kg, Day 1; 3.0 14) twice through tail vein. Urine samples were collected 21st day; divided randomly into control, adriamycin, administration...

Liver fibrosis is caused by liver injury induced a number of chronic diseases, including schistosome infection, hepatitis metabolic disease, alcoholism and cholestasis. The tissue damage occurring after or inflammation the reversible lesion; however, has become worldwide problem poses threat to human health. development an effective drug for prevention treatment ongoing uses information from different occurrences fibrosis. In present study, carbon tetrachloride (CCl4)‑induced metabonomic...