A5101841057- Oral microbiology and periodontitis research
- Anesthesia and Pain Management
- Anesthesia and Sedative Agents
- Nausea and vomiting management
- Oral and gingival health research
- Cardiac Ischemia and Reperfusion
- Bacterial biofilms and quorum sensing
- HIV/AIDS oral health manifestations
- Streptococcal Infections and Treatments
- Cardiac, Anesthesia and Surgical Outcomes
- Carbohydrate Chemistry and Synthesis
- Coronary Interventions and Diagnostics
- Pharmacological Effects and Toxicity Studies
- Cardiac Valve Diseases and Treatments
- Alkaloids: synthesis and pharmacology
- Pain Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Natural Compound Pharmacology Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Bone Metabolism and Diseases
- Cardiac electrophysiology and arrhythmias
- Synthesis and pharmacology of benzodiazepine derivatives
- Adrenal and Paraganglionic Tumors
- Plant Genetic and Mutation Studies
- Adipose Tissue and Metabolism
Hiroshima University Hospital
2021-2025
Hiroshima University
2013-2025
North Medical Center
2024
Hiroshima City Asa Citizens Hospital
2024
Shinshu University
2013
Osaka University
1988-2008
Japan Society of Chemotherapy
1988
Induction of the Escherichia coli SOS system increases ability cell to perform DNA repair and mutagenesis. Products recA umuD,C genes are required for mutagenesis induced by radiation many chemicals. Transcription including is repressed a repressor, LexA protein, derepressed proteolytic cleavage facilitated RecA protein that had been activated inducing signals produced in agents damage DNA. An form RecA, seems have roles other than its known role as an antirepressor. Derepression involved...
Porphyromonas gingivalis fimbriae are classified into six types (types I to V and Ib) based on the fimA genes encoding FimA (a subunit of fimbriae), they play a critical role in bacterial interactions with host tissues. In this study, we compared efficiencies P. strains distinct for invasion epithelial cells degradation cellular focal adhesion components, paxillin, kinase (FAK). Six representative different were tested, type II (type gingivalis) adhered invaded at significantly greater...
<title>Abstract</title> <bold>Background</bold> Remimazolam is a short-acting benzodiazepine anesthetic recommended for continuous infusion during anesthesia induction. However, the safety and efficacy of single bolus dose administration remain under investigation. This study compared with assessed administration. <bold>Methods</bold> The participants were randomly assigned into three groups based on method remimazolam day before surgery: (1) group (continuous at 12 mg/kg/h), (2) 0.1 (single...
Remimazolam is a short-acting benzodiazepine anesthetic recommended for continuous infusion during anesthesia induction. However, the safety and efficacy of single bolus dose administration remain under investigation. This study compared with assessed administration. The participants were randomly assigned into three groups based on method remimazolam day before surgery: (1) group (continuous at 12 mg/kg/h), (2) 0.1 (single mg/kg), or (3) 0.2 mg/kg). time between drug loss consciousness was...
Background: Gingival overgrowth (GO) is a serious adverse effect associated with the administration of phenytoin (PHT), PHT‐induced GO characterized by massive accumulation extracellular matrix components, especially collagen, in gingival connective tissues. However, etiology such collagen still largely unknown. We examined effects PHT on degradation process leading to human fibroblasts (HGF). Methods: HGFs were cultured various concentrations and viable cell numbers amounts determined. Gene...
Background: Periodontitis is induced by an imbalance between bacterial virulence and host defense ability. Porphyromonas gingivalis , a predominant periodontal pathogen, triggers series of inflammatory responses that aggravate the destruction periodontium. Thus, anti‐inflammatory reagents are considered desirable for effective therapy. In present study, we examined inhibitory effects hop bract polyphenol (HBP) on cellular P. membrane vesicles. Methods: Immortalized human gingival epithelial...
Partially hydrolyzed guar gum (PHGG) is a soluble dietary fiber that effective for defecation control. It influences the gut microbiota, by which it metabolized to yield short-chain fatty acids (SCFAs), and was also recently shown protect against influenza infection in humans. We here investigated effects of PHGG mouse model H1N1 virus infection. Eight-week-old C57BL/6 mice were fed normal chow with or without (500 mg/kg per day) 4 weeks, infected at 10 weeks age, analyzed 12 age....
Refractory peripheral neuropathy can occur as a side effect in 60-70% of patients receiving Paclitaxel (PTX). Yokukansan (YKS) is Japanese herbal medicine reported to have analgesic properties for entrapment nerve injuries. Therefore, we investigated the anti-allodynic on Paclitaxel-induced neuropathic pain. All experiments used 6-week-old male Sprague Dawley rats. Mechanical allodynia was evaluated using dynamic plantar aesthesiometer. A mobile touch-stimulator unit applied progressively...
Microspheres (MS) conjugated to Porphyromonas gingivalis vesicles with type II fimbriae were the most efficient human epithelial cell invaders among six types. Cell death was induced by MS, though becoming less frequent over time, invasion efficiency partially related and gingipains likely major cause.
Introduction: The program for mammalian cell growth and division consists of four successive phases; G 1 , S, 2 M. Porphyromonas gingivalis may manipulate the host cycle to benefit bacterial virulence expression, which likely causes tissue tropism observed in chronic periodontal infections. We examined P. its effects on cell‐cycle modulation mouse ST2 osteoblastic/stromal cells. Methods: Synchronized cells were infected with ATCC33277 (wild‐type, WT), gingipain‐mutants [KDP136 (Δ rgpA Δ rgpB...
Abstract Background Coronary artery disease is a leading cause of morbidity and mortality among patients with diabetes. Previously, we demonstrated that branched-chain amino acids (BCAAs) showed cardioprotective effects against cardiac ischemia/reperfusion (I/R) injury. A recent study suggested leucine (Leu), BCAA, key acid involved in mammalian target rapamycin (mTOR) activity mitochondrial function. However, whether Leu has on diabetic hearts unclear. In this study, examined the...
Porphyromonas gingivalis expresses several virulence factors such as fimbriae and proteases, termed gingipains, which are enzymes that process precursor proteins. Thus, gingipain-null mutants lack mature fimbriae. Membrane vesicle-depleted supernatants (VDS) containing soluble gingipains were prepared an exogenous gingipain fraction. Precursor proteins treated with VDS a fimbriated mutant was successfully generated. Experiments showed the wild strain adhered to invaded epithelial cells at...
Background: Severe periodontal breakdown is often associated with Down syndrome (DS); however, the etiology of this condition not understood fully. Cellular motility gingival fibroblasts a critical event for wound healing and regeneration tissues. Porphyromonas gingivalis known to be pathogen that invades host cells, contributing destruction. In study, we examined influence P. infection on DS (DGFs). Methods: DGFs normal (NGFs) were infected type II fimbriae, cellular was evaluated using an...
Náusea e Vômito no Pós‐Operatório (NVPO) é um evento adverso frequente da anestesia geral. Várias classes de antieméticos, incluindo antagonistas do receptor 5‐Hidroxitriptamina3 (5‐HT3) Neurocinina‐1 (NK‐1), têm sido utilizados para tratar a NVPO. Comparar o efeito antiemético dos NK‐1, fosaprepitanto. Foram utilizadas bases dados on‐line (PubMed, MEDLINE, Scopus, The Cochrane Library). incluídos Estudos Clínicos Randomizados (ECR) realizados em pacientes acima 18 anos classificação ASA I...
Remimazolam is a novel general anesthetic and its safety in patients with malignant hyperthermia (MH) unknown. We used myotubes derived from the skeletal muscle of MH to examine response ryanodine receptor 1 (RYR1) agonist remimazolam MH-susceptible patients. Patients underwent biopsy for Ca2+-induced Ca2+ release (CICR) rate test, diagnostic tool Japan. Ten had obtained cultures, genes associated these were analyzed. The EC50 caffeine, cresol, induce intracellular calcium concentration...
Odd‐skipped related 2 ( Osr2 ) gene is mouse homolog of Drosophila involved with the pair‐rule segmentation phenotype in mutant embryos. In this study, to examine expression regulation, promoter region was cloned and characterized, found have two enhancer elements −1463/−1031 (distal) −581/+3 (proximal) regions, a repressor (−4845/−1463, far distal). CCAAT/enhancer binding protein C/EBP sites were both distal proximal elements. activity enhanced by C/EBPδ , member family, dose‐dependent...