A5101825534- Cancer-related molecular mechanisms research
- Muscle Physiology and Disorders
- MicroRNA in disease regulation
- Peroxisome Proliferator-Activated Receptors
- Circular RNAs in diseases
- Growth Hormone and Insulin-like Growth Factors
- Animal Nutrition and Physiology
- Animal Genetics and Reproduction
- Adipose Tissue and Metabolism
- RNA regulation and disease
- RNA modifications and cancer
- Machine Learning in Bioinformatics
- Silk-based biomaterials and applications
- RNA and protein synthesis mechanisms
- Congenital heart defects research
- RNA Research and Splicing
- Neurological diseases and metabolism
- Advanced Biosensing Techniques and Applications
- Caveolin-1 and cellular processes
- Nutrition, Genetics, and Disease
- Lipid metabolism and disorders
- Cancer, Hypoxia, and Metabolism
- Microbial Metabolism and Applications
- Biochemical Analysis and Sensing Techniques
- Pharmacological Effects of Natural Compounds
Changshu Institute of Technology
2012-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2004-2022
Peking Union Medical College Hospital
2022
Soochow University
2009-2012
China Agricultural University
2006-2010
Virginia Tech
2007-2009
Tohoku University
2008
Jiangsu Agri-animal Husbandry Vocational College
2008
Northeast Agricultural University
2002-2006
Harbin Institute of Technology
2002-2004
The establishment of agricultural economies based upon domestic animals began independently in many parts the world and led to both increases human population size migration people carrying plants animals. precise circumstances earliest phases these events remain mysterious given their antiquity fact that subsequent waves migrants have often replaced first. Through use more than 1,500 modern (including 151 previously uncharacterized specimens) 18 ancient (representing six East Asian...
We report the identification of bovine miRNAs by cloning small RNAs from adipose tissue and mammary gland. Fifty‐nine distinct were identified, five them not homologous to known mammalian miRNAs, many had 3′ and/or 5′ end variants. Ribonuclease protection assays indicated that miR‐23a miR‐24, whose genes are closely located on same chromosome, co‐expressed in different tissues. The also suggested a role for several gland miR‐133, previously skeletal cardiac muscle‐specific miRNA, rumen, an...
The transcription factor signal transducer and activator of (STAT)-5 mediates GH stimulation IGF-I gene expression in the liver. Previous studies suggested that STAT5 might exert this effect by binding to an intron 2 region a distal 5′-flanking each containing two sites. Here we report identification three additional chromosomal regions total five putative sites may mediate GH-induced activation mouse By comparing 170-kb genomic DNA with corresponding human sequence, identified 19 bear...
The sterol regulatory element binding transcription factor 2 (SREBF2) gene encodes a that activates the expression of many genes involved in synthesis and uptake cholesterol, fatty acids, triglycerides, phospholipids. Through bioinformatics, we found intron 16 chicken SREBF2 might encode miR-33. Using quantitative RT-PCR, detected miR-33 variety tissues including skeletal muscle, adipose tissue, liver. Three hundred seventy eight were predicted to be potential targets chickens via miRNA...
Nonalcoholic fatty liver disease (NAFLD) is the most common metabolic in world. In this study we investigated effect of tetrahydrocurcumin (THC), a potent antioxidant, on NAFLD. We found that THC significantly reduced body weight, serum and lipids, malondialdehyde (MDA) fasting blood glucose (FBG) male NAFLD mice induced by feeding high-fat diet (HFD) for 8 weeks. A RNA-seq analysis revealed also affected expression many genes involved lipid metabolism mice, including increasing mRNA CYP51...
Growth hormone (GH) is a key regulatory factor in animal growth, development and metabolism. Based on the expression level of GH receptor, chicken liver major target organ GH, but biological effects are not fully understood. In this work we identified mRNAs miRNAs that regulated by primary hepatocytes from female chickens through RNA-seq, analyzed functional relevance these GO enrichment analysis miRNA prediction. A total 164 were found to be differentially expressed between GH-treated...
The objective of this study was to determine whether and how GH regulates hepatic expression receptor (GHR) mRNA in cattle. Ribonuclease protection assays revealed that injection a slow-release formula increased both GHR IGF-I mRNAs 1 wk after the injection. increases were highly correlated. Western blot analysis showed also liver protein level. In cattle other mammals, is expressed as variants differ 5'-untranslated region due use different promoters transcription and/or alternative...
The microRNA-126 (miR-126) is a miRNA expressed in highly vascularized tissues, and it believed to play role angiogenesis by repressing sprouty-related EVH1 domain containing 1 (Spred1). In the current study, we determined expression pattern of chicken miR-126 (gga-miR-126) predicted validated its target genes. quantitative reverse-transcription (qRT) PCR analysis showed that was various tissues with highest level lung. liver, increased from 0 7 wk age. primary hepatocytes decreased...
As the most abundant microRNA (miRNA) in liver, miR-122 plays important roles growth and development of lipid metabolism, liver diseases. Vanin 1 (VNN1) an role hepatic VNN1 may serve as a potential therapeutic target for treatment metabolic diseases caused by overactivated gluconeogenesis. In our previous RNA-seq study, we found expression increased significantly when (gga-miR-122-5p) was knocked down primary chicken hepatocytes. this verified result real-time qRT-PCR, also that highly...
Fig. S1. Nucleotide sequences of the coding region myostatin cDNA from Duck (Anas platyrhynchos), Goose (Anser anser), Pigeon (Columba livia) and Quail (Coturnix chinensi). S2. Alignment deduced amino acid for proteins. S3. Comparison E-box locations in avian gene promoter regions. Please note: The publisher is not responsible content or functionality any supporting information supplied by authors. Any queries (other than missing content) should be directed to corresponding author article.
Objective: Vanin-1 (<i>VNN1</i>) is a pantetheinase that catalyses the hydrolysis of pantetheineto produce pantothenic acid and cysteamine. Our previous studies have shown the<i>VNN1</i> specifically expressed in chicken liver which negatively regulated by microRNA-122.However, functions <i>VNN1</i> lipid metabolism haven’t been elucidated.Methods: First, we detected mRNA expression 4-week chickens were fasted 24 hours. Next, knocked out via CRISPR/Cas9...
Leptin receptor (OBR) is a member of the class I cytokine family.It signals mainly via JAK/STAT pathway and plays an important role in regulating body energy storage metabolism.This study was designed to investigate effects OBR gene on chicken growth composition.Broiler lines selected divergently for or against abdominal fat were used.Primers exon9-region using genomic sequences from public genome domain.A C/A single nucleotide polymorphism (SNP) found its three genotypes (AA, AB BB)...
Abstract Fatty acid binding proteins (FABPs) are members of a superfamily lipid-binding and occur intracellularly in vertebrates invertebrates. This study was designed to clone characterize the adipocyte fatty protein (A-FABP) gene chicken. PCR primers were according mammalian A-FABP sequence amplify partial cDNA from chicken adipose tissues, full length cloned by 5′RACE 3′RACE. Analysis showed that 74 73% homologous with porcine human gene, respectively. The similarity 77, 28, 23% at...