A5018720825- Microbial infections and disease research
- Phytoplasmas and Hemiptera pathogens
- Aquaculture disease management and microbiota
- Cocoa and Sweet Potato Agronomy
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Mycobacterium research and diagnosis
- Herpesvirus Infections and Treatments
- Studies on Chitinases and Chitosanases
- Insect symbiosis and bacterial influences
- Plant Parasitism and Resistance
- Infectious Diseases and Mycology
- Bacterial Infections and Vaccines
- Plant and Fungal Interactions Research
- Blood groups and transfusion
- Animal Virus Infections Studies
- Extracellular vesicles in disease
- Toxoplasma gondii Research Studies
- Glycosylation and Glycoproteins Research
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Mycotoxins in Agriculture and Food
- Agricultural Practices and Plant Genetics
Université Claude Bernard Lyon 1
2013-2024
Mycoplasmoses animales
2007-2024
VetAgro Sup
2013-2024
Pathophysiology, Diagnosis and Treatment of Bone Diseases
2007-2017
Mycologie et Sécurité des Aliments
2016
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2012-2014
Agence Nationale de Sécurité du Médicament et des Produits de Santé
2014
Institut National de la Recherche Agronomique
2000-2010
Fundo de Defesa da Citricultura
2002-2006
Laboratoire Génome et Développement des Plantes
2002-2006
The assembly of 20,000 sequencing reads obtained from shotgun and chromosome-specific libraries the Spiroplasma citri genome yielded 77 chromosomal contigs totaling 1,674 kbp (92%) 1,820-kbp chromosome. largest were positioned on physical genetic maps constructed pulsed-field gel electrophoresis Southern blot hybridizations. Thirty-eight annotated, resulting in 1,908 predicted coding sequences (CDS) representing an overall density only 74%. Cellular processes, cell metabolism,...
Spiroplasma citri is a plant-pathogenic mollicute. Recently, the so-called nonphytopathogenic S. mutant GMT 553 was obtained by insertion of transposon Tn4001 into first gene fructose operon. Additional operon mutants were produced either disruption or selection spontaneous xylitol-resistant strains. The behavior these spiroplasma in periwinkle plants has been studied. Plants infected via leafhoppers with wild-type strain GII-3 began to show symptoms during week following insect-transmission...
Release of extracellular vesicles (EV) by Gram-negative and positive bacteria is being frequently reported. EV are nano-sized, membrane-derived, non-self-replicating, spherical structures shed into the environment that could play a role in bacteria-host interactions. Evidence production belonging to class Mollicutes, which wall-less, mainly restricted genus Acholeplasma scanty for Mycoplasma comprises major human animal pathogens. Here release six (sub)species clinical importance was...
Abstract Background Mycoplasma bovis ( M. ) is an emerging bovine pathogen, leading to significant economic losses in the livestock industry worldwide. Infection can result a variety of clinical signs, such as arthritis, pneumonia, mastitis and keratoconjunctivitis, none which are -specific. Laboratory diagnosis therefore important. Serological tests detect antibodies considered effective indicator infection herd often used test. Combined with judgement, it also be implement control...
Mycoplasmas of the Mycoplasma mycoides cluster are all ruminant pathogens. subsp. is responsible for contagious bovine pleuropneumonia and known to produce capsular polysaccharide (CPS) exopolysaccharide (EPS). Previous studies have strongly suggested a role polysaccharides in pathogenicity. mycoides-secreted EPS was recently characterized as β(1→6)-galactofuranose homopolymer (galactan) identical product. Here, we extended characterization secreted other members M. cluster: capricolum...
Contagious bovine pleuropneumonia is a severe respiratory disease of cattle that caused by bacterium the Mycoplasma genus, namely mycoides subsp. (Mmm). In absence classical virulence determinants, pathogenicity Mmm thought to rely on intrinsic metabolic functions and specific components outer cell surface. One these latter, capsular polysaccharide galactan has been notably demonstrated play role in persistence dissemination. The free exopolysaccharides (EPS), also produced shown circulate...
Mycoplasmas are minimal, wall-less bacteria but have retained the ability to secrete complex carbohydrate polymers that constitute a glycocalyx. In members of Mycoplasma mycoides cluster, which important ruminant pathogens, glycocalyx includes both cell-attached and cell-free polysaccharides. This report explores potential secretion polysaccharides by M. agalactiae, another pathogen belongs distant phylogenetic group. Comparative genomic analyses showed agalactiae possesses all genes...
Two hundred and fifty-seven transposon Tn4001 mutants of Spiroplasma citri strain GII3 were used for transmission assays by the leafhopper vector Circulifer haematoceps into periwinkle (Catharanthus roseus) plants. Multiplication in two hosts, plant, as well symptom expression plant studied. mutants, GMT 470 553, caused no symptoms on is inserted a single copy genome these mutants. Mutant did not multiply, or multiplied only poorly, leaf-hopper was transmitted insect to nor culture medium...
In order to study the mechanism of insusceptibility Spiroplasma citri rifampin, we have cloned and sequenced its rpoB gene, which encodes beta subunit RNA polymerase. By comparison deduced amino acid sequence with sequences subunits from susceptible resistant bacteria, it was possible identify several differences in so-called Rif region (encompassing codons 500 575 Escherichia coli sequence). We constructed a chimeric gene made E. replaced by equivalent S. citri. cells harboring this were...
The role of fruR, the first gene Spiroplasma citri fructose operon, was investigated. In vivo transcription operon is greatly enhanced by presence in growth medium while glucose has no effect. When fruR not expressed, stimulated fructose, and fermentation decreased, indicating that FruR an activator operon. promoter localized primer extension, a direct T-rich repeat found to overlap -35 box. This could be binding site FruR. culture also decreases toxicity methyl alpha-glucoside, however...
Mycoplasma mycoides subsp. (Mmm) strain Afadé had previously been shown to undergo spontaneous phase variations between an opaque capsulated variant and a translucent (TR) devoid of capsule but able secrete cell-free exopolysaccharides. This variation is associated with ON/OFF genetic switch in glucose permease gene. In this study, vivo vitro assays were conducted compare the virulence two variants their abilities resist host defence. Capsulated shown, mouse model, induce longer bacteraemia...
Fructose-negative mutants of Spiroplasma citri wild-type strain GII-3 were selected by two methods. The first method is based on the selection spontaneous xylitol-resistant mutants, xylitol being a toxic fructose analogue. Five such obtained, but only one, xyl3, was unable to use and had no phosphoenolpuryvate:fructose phosphotransferase system (fructose-PTS) activity. Amplification sequencing permease gene mutant xyl3 revealed presence an adenylic insertion leading truncated permease....
Intraclonal antigenic variation in pathogenic mycoplasma species is considered an important feature of host–pathogen interaction. Such intraclonal protein was observed for the interaction Mycoplasma mycoides subsp. Small Colony, agent contagious bovine pleuropneumonia, with mAb 3F3. Colony immunostaining allows definition 3F3 ON- and OFF-type variants, which revert at low frequency. Targets were shown to be surface located, resided on multiple polypeptides 58–68 kDa size range. Phage display...
Contagious agalactia (CA) of sheep and goats caused by Mycoplasma agalactiae is a widely occurring economically important disease that difficult to control. The ELISA commonly used for the serological detection CA but it has some limitations performance available tests have not been properly evaluated. Two commercial kits are used, one involving fusion protein as target antigen other total antigen. objectives were compare these evaluating: i. Their diagnostic sensitivity specificity,...
The genus Mycoplasma, a group of free-living, wall-less prokaryotes includes more than 100 species which dozens are primary pathogens humans and domesticated animals. Mycoplasma isolated from wildlife rarely investigated but could provide fuller picture the evolutionary history diversity this genus. In 2013 several isolates wild Caprinae were tentatively assigned to new species, (M.) feriruminatoris sp. nov., characterized by an unusually rapid growth in vitro close genetic proximity...
Many mycoplasma species are isolated from the ruminant lungs as either saprophytes or true pathogens. These wall-less bacteria possess a minimal genome and reduced metabolic capabilities. Accordingly, they rely heavily on their hosts for supply of essential metabolites and, notably, peptides. Seven 13 lung-associated Mycoplasma (sub)species were shown to caseinolytic activity when grown in rich media assessed with quantitative fluorescence test. For some species, this was detected spent...
Xylella fastidiosa causes citrus variegated chlorosis (CVC), a destructive disease of citrus. forms biofilm inside plants and insect vectors. Biofilms are complex structures involving X. cells an extracellular matrix which blocks water nutrient transport in diseased plants. It is hypothesized that the might be composed polysaccharide (EPS), coded by cluster nine genes closely related to xanthan gum operon Xanthomonas campestris pv. campestris. To understand role on formation EPS...
We report here the draft genome sequences of Mycoplasma alkalescens, arginini, and bovigenitalium. These three species are regularly isolated from bovine clinical specimens, although their role in disease is unclear.
The analysis by Denaturing Gradient Gel Electrophoresis (DGGE) of the PCR-amplified V3 region 16S rRNA gene was previously shown to detect and differentiate a large number human animal mycoplasmas. In this study, we further assessed suitability technique for epidemiological surveillance mycoplasmas belonging 'Mycoplasma mycoides' cluster, phylogenetic group that includes major ruminant pathogens.The genes from approx. 50 field strains amplified analysed DGGE. Detection identification results...
Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids between cloned truncated copy and endogenous gene produced one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase used monitor expression target select cvaB mutant.