A5018678100- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- T-cell and Retrovirus Studies
- Animal Virus Infections Studies
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Blood groups and transfusion
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Mycobacterium research and diagnosis
- Cytomegalovirus and herpesvirus research
- Plant and Fungal Interactions Research
- Viral Infections and Vectors
- Pneumocystis jirovecii pneumonia detection and treatment
- Influenza Virus Research Studies
- Clostridium difficile and Clostridium perfringens research
École Nationale Vétérinaire de Toulouse
2012-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Université de Toulouse
2012-2025
Interaction Hôtes Agents Pathogènes
2014-2024
University of Liège
1991-2019
Universidad Autónoma de Madrid
1998-2014
Centro de Biología Molecular Severo Ochoa
1998-2014
Université Toulouse III - Paul Sabatier
2011
Institut National Polytechnique de Toulouse
2011
Institut National de la Recherche Agronomique
2010
Biological adaptive systems share some common features: variation among their constituent elements and continuity of core information. Some them, such as the immune system, are endowed with memory past events. In this study we provide direct evidence that evolving viral quasispecies possess a molecular in form minority components populate mutant spectra. The experiments have involved foot-and-mouth disease virus populations known evolutionary histories. composition behavior population...
ABSTRACT Cell surface molecules that can act as virus receptors may exert an important selective pressure on RNA viral quasispecies. Large population passages of foot-and-mouth disease (FMDV) in cell culture select for mutant viruses render dispensable a highly conserved Arg-Gly-Asp (RGD) motif responsible integrin receptor recognition. Here, we provide evidence viability recombinant FMDVs including Asp-143→Gly change at the RGD was conditioned by number capsid substitutions selected upon...
ABSTRACT Hypervirulent variants of foot-and-mouth disease virus (FMDV) serotype C arise upon serial cytolytic or persistent infections in cell culture. A specific mutation the internal ribosome entry site FMDV was previously associated with enhanced translation initiation activity that could contribute to hypervirulent phenotype for BHK-21 cells. Here we report several arising passage show an invariant but have a number mutations affecting structural and nonstructural viral proteins. The...
Horizontal gene transfer (HGT) is a main driving force of bacterial evolution and innovation. This phenomenon was long thought to be marginal in mycoplasmas, large group self-replicating bacteria characterized by minute genomes as result successive losses during evolution. Recent comparative genomic analyses challenged this paradigm, but the occurrence chromosomal exchanges had never been formally addressed mycoplasmas. Here, we demonstrated conjugal regions within among ruminant mycoplasma...
ABSTRACT The genetic changes selected during the adaptation of a clonal population foot-and-mouth disease virus (FMDV) to guinea pig have been analyzed. FMDV clone C-S8c1 was adapted by serial passage in animals until secondary lesions were observed. Analysis directly recovered from developed revealed selection variants with two amino acid substitutions nonstructural proteins, I 248 →T 2C and Q 44 →R 3A. On further passages, an additional mutation, L 147 →P, important antigenic site located...
Horizontal gene transfer (HGT) is a major force of microbial evolution but was long thought to be marginal in mycoplasmas. In silico detection exchanged regions and loci encoding putative Integrative Conjugative Elements (ICE) several mycoplasma genomes challenged this view, raising the prospect these simple bacteria being able conjugate. Using model pathogen Mycoplasma agalactiae, we demonstrated for first time that one elements, ICEA, indeed self-transmissible. As hallmark conjugative...
ABSTRACT Bacterial conjugation plays a pivotal role in the evolution and adaptation of genome-reduced mycoplasmas. Despite their fast rate, conjugative properties these organisms remain largely understudied, particularly vivo . In present study, ruminant pathogen Mycoplasma agalactiae was used as model organism to document mycoplasmas environments increasing complexity, from axenic cell organotypic culture conditions. Compared mating conditions, mycoplasma co-cultivation with goat epithelial...
Horizontal Gene Transfer was long thought to be marginal in Mycoplasma a large group of wall-less bacteria often portrayed as minimal cells because their reduced genomes (ca. 0.5 2.0 Mb) and limited metabolic pathways. This view recently challenged by the discovery conjugative exchanges chromosomal fragments that equally affected all parts chromosome via an unconventional mechanism, so whole mycoplasma genome is potentially mobile. By combining next generation sequencing classical mating...
Concurrent infections with multiple pathogens are often described in cattle respiratory illness. However, how the host-pathogen interactions influence clinical outcome has been only partially explored this species. Influenza D virus (IDV) was discovered 2011. Since then, IDV detected worldwide different hosts. A significant association between and bacterial sick shown epidemiological studies, especially Mycoplasma bovis. In an experimental challenge, aggravated M. bovis-induced pneumonia....
// Farhan Anwar Khan 1,2,3,* , Muhammad Faisal 1,2,* Jin Chao 1,2 Kai Liu Xi Chen 2 Gang Zhao Harish Menghwar Hui Zhang Xifang Zhu Asif Rasheed Chenfei He Changmin Hu Yingyu 1,4 Eric Baranowski 6,7 Huanchun 1,2,4 and Aizhen Guo 1,2,4,5 1 The State Key Laboratory of Agricultural Microbiology, Huazhong University, Wuhan, China College Veterinary Medicine, 3 Department Animal Health, University Agriculture, Peshawar, Pakistan 4 Development Diagnostic Products, Ministry International Joint...
Bovine respiratory disease (BRD) is among the most prevalent diseases in young cattle. BRD due to complex interactions between viruses and/or bacteria, of which have a moderate individual pathogenicity.
We present sequence data from two genomic regions of foot-and-mouth disease virus (FMDV) subjected to several experimental passage regimens. Maximum-likelihood estimates the nonsynonymous-to-synonymous rate ratio parameter (dN/dS) suggested action positive selection on some antigenic sites FMDV capsid during passages. These showed an accumulation convergent amino acid replacements massive serial cytolytic passages and also in persistent infections cell culture. This was most significant at...