Abstract Background Neutrophil‐to‐lymphocyte ratio (NLR) and platelet‐to‐lymphocyte (PLR) have been described as prognostic markers in cancer inflammatory diseases. The use of these measurements dogs cats with acute pancreatitis has not yet investigated. Objective objective was to evaluate NLR PLR for usefulness biomarkers severity clinical course cats. Methods In this prospective study, we investigated 67 41 17 healthy controls. We compared the control animals. disease measured using a...

Mammary tumors are the most frequent cancers in female dogs exhibiting a variety of histopathological differences. There is lack knowledge about genomes these common dog tumors. Five three different histological subtypes were evaluated. Massive parallel sequencing (MPS) was performed comparison to respective somatic genome each animal. Copy number and structural aberrations validated using droplet digital PCR (ddPCR). Using mate-pair chromosomal aneuploidies found two tumors, smaller...

Abstract Monitoring large carnivores is imperative for conservation planning, but difficult due to their elusive behaviour and natural rarity. Some such as the cheetah ( Acinonyx jubatus ) are particularly wide ranging often go undetected despite being present, or detected at rates too low make meaningful quantitative inferences. The combination of minimally invasive survey techniques, detection dog surveys camera traps, holds promise improving monitoring efforts carnivores. We surveyed a...

Magnetic resonance imaging (MRI) is widely used in human medicine, offering multiple contrast mechanisms to visualise different tissue types. It also gaining importance veterinary including diagnosing joint disorders. The menisci of the stifle play a crucial role development osteoarthritis (OA), and multi-parameter MRI may aid early OA diagnosis, potentially improving therapeutic outcomes. In previous ex vivo study, we measured T2 relaxation times elderly dogs with mild histological signs...

In recent years, the European companion animal veterinary care market has experienced significant growth, with 166 million households (50%) owning one or more of Europe’s 352 animals. This expansion is accompanied by a notable trend toward corporatisation practices, particularly in urban areas and certain countries. Corporatisation reshaped profession, offering benefits such as economies scale, advanced treatments, structured career pathways for early-career veterinarians. However, this...

Abstract Wildlife detection dogs facilitate scat sample collection for wide‐ranging species such as many large carnivores. Performance evaluations are required to effectively compare dog teams. Key metrics evaluation the ratio of targets detected (sensitivity) and ability discriminate target scent (precision). These their predictors commonly evaluated through experimental assessments; however, extent which these can inform field performance has not been extensively explored. We analyzed 4...

Background The most common nasal cavity disease (ND) in dogs is the malignant tumor. Prognosis and survival after radiation therapy are reported to correlate with tumor size, therefore indirectly time diagnosis. Diagnosis of a requires imaging procedures under anesthesia. Thus, diagnostic serum markers urgently needed for early detection therapeutic monitoring. Materials methods This prospective, blinded study included discharge that completed comprehensive workup ND. Dogs were evaluated by...

Abstract Objective —To compare response rates and remission survival times in dogs with lymphoma treated a continuous, multiagent, doxorubicin-based chemotherapeutic protocol or short-term single-agent incorporating doxorubicin. Design —Nonrandomized controlled clinical trial. Animals —114 lymphoma. Procedures —Dogs were consisting of L-asparaginase, vincristine, cyclophosphamide, doxorubicin, methotrexate, prednisolone (n = 87) doxorubicin alone (27). Results —63 86 (73%) the multiagent...

Hepatitis E virus (HEV) is a human pathogen that primarily transmitted by the fecal-oral route and causes usually self-limiting acute viral hepatitis. The endemic in developing countries of Africa, Asia, Latin America responsible for sporadic cases industrialized countries. In western Europe, an increasing number autochthonous have been associated with zoonotic transmissions HEV from domestic wild animals. Germany, animal reservoirs mainly assigned to pigs boars. To investigate potential...

Abstract Background A multimodal approach for diagnostic tests under anesthesia is required to diagnose nasal cavity pathology (NP) reliably in dogs. Blood test results may provide clues the suspected NP. Methods This prospective blinded study assessed 72 dogs with chronic discharge due NPs, and 10 healthy as control group (CG). NPs were diagnosed using whole-body computed tomography (CT), upper airway endoscopy, examination of mucosal swabs by bacterial fungal culture, histopathological...

Interleukin-8 (IL-8 or CXCL8) is a highly selective pro-inflammatory chemokine, that elevated in sera of humans and animals with various inflammatory diseases. CXCL8 possibly involved uncontrolled inflammation the development systemic response syndrome (SIRS) sepsis. Nevertheless, its behavior precise properties course are not fully understood. Thus, we used naturally occurring canine pyometra as model inflammation, order to examine serum relation disease intensity commonly analyzed...

Endoluminal gastroplication, using the EndoCinch procedure, has emerged as a potential endoscopic antireflux therapy. Although initial results have been promising, long-term durability of treatment is uncertain due to suture loss. A new suturing device, "ESD," developed that promises excellent visibility and control. The aim this study was evaluate prospectively feasibility efficacy ESD method after failure.The involved 20 patients with gastroesophageal reflux disease (GERD), who had...

Background Interleukin‐6 ( IL ‐6) is involved in the acute phase reaction during inflammatory disease, including septicemia and response syndrome SIRS ). In people, ‐6 has been studied as a prognostic marker intensive care medicine, where nonsurvivors had significantly higher IL‐6 concentrations than survivors. Objectives The objective of study was to investigate value dogs admitted unit based on sensitivity specificity. Methods Dogs suffering from different severe diseases treated were...

A somatic deletion at the proximal end of canine chromosome 27 (CFA27) was recently reported in 50% malignant mammary tumors. This region harbours tumor suppressor gene prefoldin subunit 5 (PFDN5) and correlated with a higher Ki-67 score. PFDN5 has been described to repress c-MYC is, therefore, candidate tumor-suppressor cancer-driver cancer. Aim this study confirm recurrent larger number tumors.Droplet digital PCR for performed DNA from 102 malignant, 40 benign tumors/dysplasias, 11...

Mastitis is one of the most common diseases dairy cows. Procalcitonin (PCT) has been described as an endogenous inflammatory biomarker for bacterial infections. The aim this study was to find possible correlations between PCT concentrations in serum and milk cows with mastitis their clinical signs disease progression. In total, 88 were examined, which 30 animals diagnosed mastitis, had subclinical 28 designated a healthy control group. diseased re-examined after 12 days. All levels...

Background: The aim of this study was to substantiate the ability noninvasive low-field magnetic resonance imaging (lfMRI) accurately depict meniscal lesions, thereby establishing lfMRI as a viable option for diagnosis pathology. Study Design: This prospective, controlled study. Materials and Methods: In each 57 stifle joints, 5 predefined sequences using 0.25 T were used. scans evaluated by an experienced surgeon (DECVS) through external assessment radiologist (DECVDI). patients divided...

Because essential amino acids are metabolized in the liver, liver diseases may impair their catabolism. In this study, serum L-phenylalanine concentrations 28 dogs with were compared those of healthy and 13 nonhepatic diseases. Dogs had significantly increased to (P<0.001) (P<0.01). There no significant differences among different degrees The sensitivity specificity fasting bile comparable.

Cytokines are part of pathogenesis in many diseases. Their measurement could be interesting for diagnostic purposes. One cytokine which participates different inflammatory and neoplastic diseases is interleukin-6 (IL-6). The aim this study was to investigate the IL-6 serum concentration dogs with liver show if there any association between level disease aetiology or degree disease. measured acute hepatitis, chronic hepatitis degrees primary secondary tumours. data were compared clinically...

Background: Cytokines are mediators of disease. Expression levels in the blood could be clinical relevance. Objective: Aim this study was to show if serum IL-1β any relevance concerning dogs. measured samples healthy dogs find a reference range for individuals. Measurements should substance possible marker early stages inflammation. Therefore, relation between and grades leukocytosis analyzed. Methods: concentrations were assessed by use human enzyme linked immunosorbent assay (ELISA). 39...

Acute diarrhea is a common clinical condition where parameters are used to assess disease severity, course, and prognosis.The aim of this study was investigate procalcitonin (PCT) beta-defensin2 (Bdef2) as biomarkers for prognosis dogs with acute diarrhea.Dogs (enteritis group [EG], n = 35) were compared 30 healthy controls. The in the EG scored using Canine Diarrhea Severity (CADS) index grouped by bacterial fecal culture results. Procalcitonin Bdef2 analyzed serum feces.Dogs showed higher...

Osteoarthritis is a chronic disease that often affects the canine stifle joint. Due to their biomechanical function, menisci in play an important role osteoarthritis. They compensate for incongruence joint and distribute minimize compressive loads, protecting hyaline articular cartilage from damage. Meniscal degeneration favors development progression of Qualitative magnetic resonance imaging (MRI) current golden standard detecting meniscal changes, but it has limitations early signs...