A5087815300- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- S100 Proteins and Annexins
- Proteoglycans and glycosaminoglycans research
- Immunotherapy and Immune Responses
- Chemical Synthesis and Analysis
- Invertebrate Immune Response Mechanisms
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Complement system in diseases
- Immune Response and Inflammation
- Radiopharmaceutical Chemistry and Applications
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Barrier Structure and Function Studies
- Gastrointestinal Tumor Research and Treatment
- Signaling Pathways in Disease
- Malaria Research and Control
- RNA modifications and cancer
- Computational Drug Discovery Methods
- Boron Compounds in Chemistry
- Toxoplasma gondii Research Studies
Kyoto University
2007-2024
Kyoto Pharmaceutical University
2007-2024
Obihiro University of Agriculture and Veterinary Medicine
2018-2021
National Institute of Advanced Industrial Science and Technology
2015-2021
Sanford Burnham Prebys Medical Discovery Institute
2012-2020
Ritsumeikan University
2007-2020
Discovery Institute
2020
Japan Society for the Promotion of Science
2012
Agrobiotechnology Institute
2008-2009
Abstract Dendritic cells (DCs) are APCs that play an essential role by bridging innate and adaptive immunity. DC-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) is one of the major C-type lectins expressed on DCs exhibits high affinity for nonsialylated Lewis (Le) glycans. Recently, we reported characterization oligosaccharide ligands SW1116, a typical human colorectal carcinoma recognized mannan-binding protein, which serum lectin has similar...
We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic (hES) cells by using hiPS (Tic) as an antigen, followed differential screening of mouse hybridomas with and embryonal carcinoma (hEC) cells. Upon western blotting R-10G, hiPS/ES cell lysates gave single but unusually diffuse band at position corresponding >250 kDa. The antigen protein was isolated from the (iPS) affinity column R-10G. R-10G positive resistant digestion peptide...
Immunogenicity is a major caveat of protein therapeutics. In particular, the long-term administration therapeutic agents leads to generation antidrug antibodies (ADAs), which reduce drug efficacy while eliciting adverse events. One promising solution this issue use mirror-image proteins consisting d-amino acids, are resistant proteolytic degradation in immune cells. We have recently reported chemical synthesis enantiomeric form variable domain antibody heavy chain (d-VHH). However,...
Malaria is a major infectious disease in the world. In 2015, approximately 212 million people were infected and 429,000 killed by this disease. Plasmodium falciparum, which causes falciparum malaria, becoming resistant to artemisinin (ART) Southeast Asia; therefore, new anti-malarial drugs are urgently needed. Some excellent drugs, such as quinine or ART, originally obtained from natural plants. Hence, authors screened product library comprising traditional Chinese medicines (TCMs) identify...
This study reports the determination of carbohydrate epitope monoclonal antibody F77 previously raised against human prostate cancer PC-3 cells (Zhang, G., Zhang, H., Wang, Q., Lal, P., Carroll, A. M., de la Llera-Moya, Xu, X., and Greene, M. I. (2010) Proc. Natl. Acad. Sci. U. S. 107, 732–737). We performed a series co-transfections using mammalian expression vectors encoding specific glycosyltransferases. thereby identified branching enzymes FUT1 (required for Fucα1→2Gal linkage) as being...
Mirror-image proteins (d-proteins) are promising scaffolds for drug discovery because of their high proteolytic stability and low immunogenic properties. Facile reproducible processes the preparation functional d-proteins required application in therapeutic biologics. In this study, we designed synthesized a novel monobody variant with two cysteine substitutions that facilitate synthetic process via sequential native chemical ligations improve protein by disulfide bond formation. The...
Significance Asthmatic inflammation is orchestrated by T-lymphocyte cell trafficking network within lungs, blood circulation, secondary lymphoid organ, and peripheral tissue. Here, we demonstrated that T following eosinophil recruitment was substantially reduced in our recently generated mouse model, where heparan sulfate synthase exostoses-1 (Ext1) knockout an inducible manner. Moreover, discovered even a monosaccharide, 2,4-disulfated iduronic acid (Di-S-IdoA), bound to chemokine CCL20...
Abstract Jacalin, an α-O-glycoside of the disaccharide Thomsen-Friedenreich antigen (galactose β1-3 N-acetylgalactosamine, T-antigen)-specific lectin from jackfruit seeds, has been shown to induce mitogenic responses and block infection by HIV-1 in CD4+ T lymphocytes. The molecular mechanism underlying Jacalin-induced cell activation not elucidated completely yet. In present study, protein tyrosine phosphatase (PTPase) CD45 was isolated a Jurkat membrane fraction as major receptor for...
Mannan (mannose)-binding protein (MBP) is a C-type serum lectin that plays key role in innate immunity. MBP forms large multimers (200-600 kDa) and exhibits broad specificity for mannose, N-acetylglucosamine, fucose. high affinity unique oligosaccharides have been isolated from human colorectal carcinoma (SW1116) cells characterized as highly fucosylated m.w. type 1 Lewis glycans. In this study, we first demonstrated recognizes primary tissues through tumor-associated ligands. We performed...
We have generated a mouse monoclonal antibody (R-17F, IgG1 subtype) specific to human induced pluripotent stem (hiPS)/embryonic (ES) cells by using hiPS cell line as an antigen. Triple-color confocal immunostaining images of with R-17F indicated that the epitope was expressed exclusively and intensively on membranes co-localized partially those SSEA-4 SSEA-3. Lines evidence suggested predominant part glycolipid. Upon TLC blot total lipid extracts from R-17F, one major R-17F-positive band...
Immunogenic responses by protein therapeutics often lead to reduced therapeutic effects and/or adverse via the generation of neutralizing antibodies antidrug (ADA). Mirror-image proteins variable domain heavy chain antibody (VHH) are potential novel with high-affinity binding target and immunogenicity because these mirror-image VHHs (d-VHHs) less susceptible proteolytic degradation in antigen-presenting cells (APCs). In this study, we investigated preparation protocols d-VHHs their...
<title>Abstract</title> Engineered protein scaffolds function as binders against various target molecules with high affinity and specificity comparable to conventional IgG antibodies. However, biologically produced drugs are generally degraded by proteases often exhibit immunogenicity. To increase protease resistance decrease immunogenicity of peptides proteins, mirror-image peptide/protein consisting D-amino acids have been developed so far mainly through the phage display technique. Here,...
Mannan-binding protein (MBP) is a C-type mammalian lectin specific for mannose and N-acetylglucosamine. MBP mainly synthesized in the liver occurs naturally two forms, serum (S-MBP) intracellular (I-MBP). S-MBP activates complement association with MBP-associated serine proteases via pathway. Despite our previous study (Mori, K., Kawasaki, T., Yamashina, I. (1984) Arch. Biochem. Biophys. 232, 223-233), subcellular localization of I-MBP its functional implication have not been clarified yet....
Abstract Serum C-type lectin MBP is shown to bind DNA and RNA from bacteria, plasmids, synthetic oligonucleotides, fragmented of apoptotic cells via its carbohydrate recognition domain. MBP, also known as MBL, a that be soluble host defense factor involved in innate immunity. It has been well established dying microbes release highly viscous induces inflammation septic shock, display on their surfaces. However, PRRs mediate the clearance free have not characterized clearly. Although was...
Abstract Background Annexin A1 is expressed specifically on the tumour vasculature surface. Intravenously injected IF7 targets via annexin A1. We tested hypothesis that overcomes blood–brain barrier and intravenously IF7C(RR)-SN38 eradicates brain tumours in mouse. Methods (1) A dual-tumour model was generated by inoculating luciferase-expressing melanoma B16 cell line, B16-Luc, into under skin of syngeneic C57BL/6 mice. daily at 7.0 μmoles/kg growth assessed chemiluminescence using an IVIS...
Cryptosporidium and Toxoplasma are parasites that have caused problems worldwide. causes severe watery diarrhoea may be fatal in immunocompromised patients infants. Nitazoxanide is the only agent currently approved by FDA, but its efficacy limited. Toxoplasmosis also a problem immunocompromised, as available treatment options limited patient tolerance can poor. In present investigation, we screened libraries of epigenetic compounds to identify those inhibited C. parvum growth. Nullscript was...
Abstract Background p -Boronophenylalanine ( 10 BPA) is a powerful B drug used in current clinical trials of BNCT. For BNCT to be successful, high (500 mg/kg) dose BPA must administered over few hours. Here, we report efficacy after rapid, ultralow-dose administration either tumor vasculature-specific annexin A1-targeting IFLLWQR (IF7)-conjugated or borocaptate sodium BSH). Methods (1) IF7 conjugates drugs intravenously injected into MBT2 bladder tumor-bearing mice and biodistribution tumors...
Summary It has been well established that CD45 is a key receptor‐type protein tyrosine phosphatase (PTPase) regulating Src‐family kinase (Src‐PTK) in T and B lymphocytes. However, precisely how exerts its effect these lymphocytes remains controversial. We recently reported Jacalin, an α‐ O ‐glycoside of the disaccharide Thomsen–Friedenreich antigen‐specific lectin from jackfruit seeds, caused marked T‐cell activation response to receptor ligation CD28 costimulation by binding CD45. On...
Malaria remains one of the most important infectious diseases in world. In 2017 alone, approximately 219 million people were infected with malaria, and 435,000 died this disease. Plasmodium falciparum, which causes falciparum is becoming resistant to artemisinin (ART) Southeast Asia; therefore, new antimalarial drugs are urgently needed. Some excellent drugs, such as quinine ART, originally obtained from plants. Hence, we analyzed effects marine natural products find agents. We used a...
T7 phage libraries displaying random peptides are powerful tools for screening peptide sequences that bind to various target molecules. The system has the advantage of less biased distribution compared M13 system. However, construction DNA is challenging due its long 36 kb linear DNA. Furthermore, diversity depends strongly on efficiency commercially available packaging extracts. To address these issues, we examined combination seamless cloning with cell-free translation systems. Seamless...
Abstract This report describes the isolation and characterization of two new antibodies, R-6C (IgM) R-13E (IgM), which were generated in C57BL/6 mice (Mus musculus) using Tic (JCRB1331) human induced pluripotent cell (hiPSC) line as an antigen, their comparisons with existing R-10G (IgG1) R-17F (IgG1). Their epitopes studied by western blotting after various glycosidase digestions, binding analyses enzyme-linked immunosorbent assays (ELISAs) microarrays synthetic oligosaccharides. The...