A5061815402- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Carbohydrate Chemistry and Synthesis
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- Photochromic and Fluorescence Chemistry
- Endoplasmic Reticulum Stress and Disease
- Biochemical and Molecular Research
- Cellular transport and secretion
- Bacterial Genetics and Biotechnology
- Machine Learning in Bioinformatics
Nagoya University
2019-2024
The University of Tokyo
2013-2024
Japan Science and Technology Agency
2020-2023
Center for High Pressure Science and Technology Advanced Research
2021
Graduate School USA
2020
Bunkyo University
2016
Tohoku University
2014
Osaka University
2006-2012
Kyoto University
2005-2006
Abstract Tank-binding kinase 1 (TBK1) is a Ser/Thr that involved in many intracellular processes, such as innate immunity, cell cycle, and apoptosis. TBK1 also important for phosphorylating the autophagy adaptors mediate selective autophagic removal of damaged mitochondria. However, mechanism by which PINK1-Parkin-mediated mitophagy activates remains largely unknown. Here, we show adaptor optineurin (OPTN) provides unique platform activation. Both OPTN-ubiquitin OPTN-pre-autophagosomal...
DNA repair proteins can be recruited by their histone reader domains to specific epigenomic features, with consequences on intragenomic mutation rate variation. Here, we investigated H3K4me1-associated hypomutation in plants. We first examined 2 which, plants, contain Tudor domains: PRECOCIOUS DISSOCIATION OF SISTERS 5 (PDS5C), involved homology-directed repair, and MUTS HOMOLOG 6 (MSH6), a mismatch protein. The MSH6 domain of Arabidopsis (Arabidopsis thaliana) binds H3K4me1 as previously...
SARS-CoV-2–neutralizing antibody-like proteins were generated by a high-speed in vitro selection method.
In the summer of 2012, 1 year after nuclear accident in March 2011 at Fukushima Daiichi power plant, we examined effects gamma radiation on rice a highly contaminated field Iitate village Fukushima, Japan. We investigated morphological and molecular changes healthy seedlings exposed to continuous low-dose up 4 µSv h−1, about 80 times higher than natural background level. After exposure rays, expression profiles selected genes involved DNA replication/repair, oxidative stress, photosynthesis,...
The discovery of riboswitching has accelerated research on the interaction between RNA and small organic compounds. It will be important for biologists to artificially reversibly control gene expression in vivo through molecules. In this paper, we report that aptamers obtained from vitro selection which a photoresponsive short peptide containing azobenzene moiety with flanking arginine residues both sides as ligand provided reversible binding immobilized onto gold surface. We designed...
One-pot multiple peptide ligation is a key technology to improve the efficiency of chemical protein synthesis. repetitive requires cycle three steps: ligation, removal protecting group, and inactivation deprotection reagent. However, previous strategies are not sufficient because harsh conditions, slow rates, difficulty in quenching To address these issues, we developed rapid, efficient subsequent strategy using an allyloxycarbonyl group protect N-terminal cysteine residue....
The N-terminal tails of histones protrude from the nucleosome core and are target sites for histone modifications, such as acetylation methylation. Histone is considered to enhance transcription in chromatin. However, contribution tail by RNA polymerase II (RNAPII) has not been clarified. In present study, we reconstituted nucleosomes lacking each histone, H2A, H2B, H3 or H4, performed RNAPII assays. We found that H3, but H2B functions pausing at SHL(-5) position nucleosome. Consistently,...
Abstract A chemically-activatable alkynyl steroid analogue probe has been synthesized for visualizing the lipid raft membrane domains by Raman microscopy. The probe, in which ring of its backbone is replaced with an group, was designed to enable activation alkyne signal through Eschenmoser-Tanabe fragmentation reaction oxidized cholesterol precursor bilayer membranes. observed form liquid-ordered raft-like on a model giant-liposome system similar manner as cholesterol, and large accumulated...
5-Hydroxymethylcytosine (hmC) is an essential intermediate in the active DNA demethylation pathway. Here we report a new base-resolution method for measuring hmC by combining peroxotungstate-mediated oxidation and sequencing analysis. We reveal that oxidized product of hmC, trihydroxylated thymine (thT), tolerated incorporation dATP as substrate process polymerase elongation. By comparing results Sanger before after oxidation, observed sites on single-stranded DNAs could be discriminated...
Difficult peptide ligation between Asp and Cys subsequent deprotection proceeded in one pot by adding a small amount of Pd/TPPTS complex.
Chemical protein synthesis assisted by an organoruthenium catalyst streamlined the production of heterochromatin factors bearing various patterns epigenetic modifications, and their biological significance was elucidated.
Abstract Strategies for one‐pot peptide ligation enable chemists to access synthetic proteins at a high yield in short time. Herein, we report novel multi‐segments strategy using N‐terminal thiazolidine (Thz) and newly designed formaldehyde scavenger. Among the 2‐aminobenzamide‐based aldehyde scavengers, 2‐amino‐5‐methoxy‐ N ′, ′‐dimethylbenzohydrazide (AMDBH) can remarkably convert Thz into unprotected cysteine pH 4.0. Furthermore, AMDBH degrades considerably low rate 7.5, thioester...
Aminoacylation of tRNA is an essential event in the translation system. Although modern system protein enzymes play sole role aminoacylation, primitive RNA molecules could have catalysed aminoacylation onto or tRNA-like molecules. Even though such so far are not identified from known organisms, vitro selection has generated catalysts a pool random sequences. Among them, set sequences, referred to as flexizymes (Fxs), discovered our laboratory able charge amino acids tRNAs. Significantly, Fxs...
The chemical synthetic route to histone H2A is described. An H2A–H2B dimer, octamer, and nucleosome were reconstituted with the H2A.
Abstract Ubiquitin-like with PHD and RING finger domain-containing protein 1 (UHRF1)-dependent DNA methylation is essential for maintaining cell fate during proliferation. Developmental pluripotency-associated 3 (DPPA3) an intrinsically disordered that specifically interacts UHRF1 promotes passive demethylation by inhibiting chromatin localization. However, the molecular basis of how DPPA3 inhibits remains unclear. We aimed to determine structure mouse plant homeodomain (PHD) complexed using...
<title>Abstract</title> Engineered protein scaffolds function as binders against various target molecules with high affinity and specificity comparable to conventional IgG antibodies. However, biologically produced drugs are generally degraded by proteases often exhibit immunogenicity. To increase protease resistance decrease immunogenicity of peptides proteins, mirror-image peptide/protein consisting D-amino acids have been developed so far mainly through the phage display technique. Here,...
Modulation of biological networks assembled by diverse interactions among biologically active molecules has provided a platform for innovative biotechnologies. Here, we report RNA aptamers that bind to photoresponsive peptide (KRAzR; Lys-Arg-azobenzene-Arg) containing azobenzene chromophore, which can change its structure photoirradiation. Aptamers were identified after 10 cycles an in vitro selection procedure starting with DNA library 70 nt random region. Surface plasmon resonance (SPR)...
Histone H2A and H2B form a H2A–H2B heterodimer, which is fundamental unit of nucleosome assembly disassembly. Several posttranslational modifications change the interface between dimer H3–H4 tetramer regulate stability. However, associated with have not been discussed. In this paper, it shown that Tyr57 phosphorylation in strongly influences dimerization. Tyr57-phosphorylated was chemically synthesized utilized to reconstitute as well canonical H2A. Thermal shift assays showed destabilized...
We report selective removals of N-terminal and internal Cys protecting groups using different palladium complexes to facilitate the efficient chemical protein synthesis. Utilizing orthogonal deprotection pairs, we accomplished synthesis histone H3 containing trimethylated Lys through combination Pd(0)-mediated Alloc for one-pot multiple peptide ligation Pd(II)Cl2-mediated Acm recover native residues after desulfurization.